Detailed information of oriT

oriT


The information of the oriT region


oriTDB ID   101019
Name   oriT2_pWBG762 experimental
Organism   Staphylococcus aureus
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   GQ900474 (6517..6706 [+], 190 nt)
oriT length   190 nt
IRs (inverted repeats)      IR1: 133..139, 143..149  (GTCTGGC..GCCAGAC)
  IR2: 150..156, 163..169  (CTATTAT..ATGATAG)
  IR3: 128..132, 173..177  (GGAAT..ATTCT)
Location of nic site      _
Conserved sequence flanking the
  nic site  
 
 _
Note   

  oriT sequence  


Download         Length: 190 nt

>oriT2_pWBG762
TGTCTTTTTTATTGATTTTTTGTAAAAAAGTACTGTCTTATTTTTGTGACAAATGCTGTATGTAGTGTCACAAAAGTGTGACACTACAGCTTTTTATGATATCACTTTAAAATTACTTAAAACCCTTGGAATGTCTGGCTTTGCCAGACCTATTATTTTTGAATGATAGCAAATTCTCCTTATGCTCTTA

Visualization of oriT structure (The oriT was characterized experimentally)

  oriT secondary structure

Predicted by RNAfold.

Download structure file

  Reference


[1] Frances G O'Brien et al. (2015) Origin-of-transfer sequences facilitate mobilisation of non-conjugative antimicrobial-resistance plasmids in Staphylococcus aureus. Nucleic acids research. 43(16):7971-83. [PMID:26243776]


Host bacterium


ID   1480 GenBank   GQ900474
Plasmid name   pWBG761 Incompatibility group   -
Plasmid size   26838 bp Coordinate of oriT [Strand]   6517..6706 [+]
Host baterium   Staphylococcus aureus

Cargo genes


Drug resistance gene   resistance to amoxicillin, ampicillin, penicillin and piperacillin
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrIIA21