Detailed information of oriT

oriT


The information of the oriT region


oriTDB ID   101018
Name   oriT1_pWBG762 experimental
Organism   Staphylococcus aureus
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   GQ900475 (7332..7520 [-], 189 nt)
oriT length   189 nt
IRs (inverted repeats)      IR1: 118..124, 129..135  (CCCCATT..GATGGGG)
  IR2: 100..106, 110..116  (ATCTGGC..GCCAGAT)
  IR3: 83..88, 93..98  (GGAAT..-TTCC)
Location of nic site      _
Conserved sequence flanking the
  nic site  
 
 _
Note   

  oriT sequence  


Download         Length: 189 nt

>oriT1_pWBG762
TGTCTTATTTTTGTGACAAATGCTGTATGTAGTGTCACAAAAGTGTGACACTTGAGCTTTTTATGACCCCACACAAAAACATTTTCGAAGCCTTGAAATATCTGGCTTTGCCAGATCCCCCATTCATTGATGGGGACAAATTTCCCTTATGCTCTTACGGAGATTTTAGAGTTAAATTAAAATTTCTAA

Visualization of oriT structure (The oriT was characterized experimentally)

  oriT secondary structure

Predicted by RNAfold.

Download structure file

  Reference


[1] Frances G O'Brien et al. (2015) Origin-of-transfer sequences facilitate mobilisation of non-conjugative antimicrobial-resistance plasmids in Staphylococcus aureus. Nucleic acids research. 43(16):7971-83. [PMID:26243776]


Host bacterium


ID   1479 GenBank   GQ900475
Plasmid name   pWBG762 Incompatibility group   -
Plasmid size   54023 bp Coordinate of oriT [Strand]   7332..7520 [-]
Host baterium   Staphylococcus aureus

Cargo genes


Drug resistance gene   resistance to amoxicillin, ampicillin, penicillin and piperacillin
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrIIA21