Detailed information of oriT

oriT


The information of the oriT region


oriTDB ID   101016
Name   oriT_pWBG744 experimental
Organism   Staphylococcus aureus
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NC_018972 (661..849 [+], 189 nt)
oriT length   189 nt
IRs (inverted repeats)      IR1: 132..138, 142..148  (GTCTGGC..GCCAGAC)
  IR2: 149..155, 162..168  (CTATCAT..ATGATAG)
  IR3: 127..131, 172..176  (AGAAT..ATTCT)
Location of nic site      _
Conserved sequence flanking the
  nic site  
 
 _
Note   

  oriT sequence  


Download         Length: 189 nt

>oriT_pWBG744
TGTCTTATTTTTGTGACAAATGCTGTATGTAGTGTCTCAAAACTGTGACAACCGCAATATATTGTGTCACAAAAGCGTGACACTACAGCTTTTTATGATATCACTTTAAAATTACTTAAAACCCTTAGAATGTCTGGCTTTGCCAGACCTATCATTTTTGAATGATAGCAAATTCTCCTTATGCTCTTA

Visualization of oriT structure (The oriT was characterized experimentally)

  oriT secondary structure

Predicted by RNAfold.

Download structure file

  Reference


[1] Frances G O'Brien et al. (2015) Origin-of-transfer sequences facilitate mobilisation of non-conjugative antimicrobial-resistance plasmids in Staphylococcus aureus. Nucleic acids research. 43(16):7971-83. [PMID:26243776]


Host bacterium


ID   1477 GenBank   NC_018972
Plasmid name   pWBG744 Incompatibility group   -
Plasmid size   27268 bp Coordinate of oriT [Strand]   661..849 [+]
Host baterium   Staphylococcus aureus

Cargo genes


Drug resistance gene   blaZ
Virulence gene   sed
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrIIA21