Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 101013 |
| Name | oriT_Ptw  |
| Organism | Burkholderia cenocepacia J2315 |
| Sequence Completeness | intact |
| NCBI accession of oriT (coordinates [strand]) | NC_011003 (65490..66190 [+], 701 nt) |
| oriT length | 701 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note |
oriT sequence
Download Length: 701 nt
>oriT_Ptw
GAAAAACGCCTGGAATGAAAAGAGCGGGCGCTCGTGGAGTCGAGGAAAAACCTCGACTCGCGCACGAAACCGCGCTCGATTACGGCCCTCATGACTGCATTATTTTGAGCGACAGTAGCATGGCGACGAGCGTTTCGCAATAACCCATTTATTTTCATAGTGATACTTATCGAATGTCAGACACACCCGTTCTAGCTGTCTGACAACGTGGCCCAATCATGACGGGGCGGGCGCGACATACTGGCAATGTTCGCGCTTTCCGGTCATTCGGGTGTCTGACATCGCGAGCCTTAACGCAAGCCGTTTCTCGCGATACGTGGCGGCGTTTTCTCGACAGGTCGACGGTGGCTGTCTGACGCTGCAAACCGCTACCGTCAGTGGTCGAGCGGGAATTTTCTATTCCTCTGGAGTCCGAATTATATTCTCCGATTCCGAATTTGTCTGACACCGGAGGATATGACGTACAAGGGGTCGCGCTTGATCCCTGCCTCGCTTTTTTCGCCTATTTTCGTAGGTGTCTGACGCCGAAGGCACCGCGCCATACGGCGCGACACACCGCAACAACGTTGCATGGTGTGTGACGATCGTCTTAGGGGGCGGGGATTCCCCCGCTTCCTAAGACGATCGTAGCCAACTTCACTTCAGGTTTGGGCCGGTCGCGGAGCCTTCACCCGGTAAGGTACGGCGGTTCCCGCTCAAGG
GAAAAACGCCTGGAATGAAAAGAGCGGGCGCTCGTGGAGTCGAGGAAAAACCTCGACTCGCGCACGAAACCGCGCTCGATTACGGCCCTCATGACTGCATTATTTTGAGCGACAGTAGCATGGCGACGAGCGTTTCGCAATAACCCATTTATTTTCATAGTGATACTTATCGAATGTCAGACACACCCGTTCTAGCTGTCTGACAACGTGGCCCAATCATGACGGGGCGGGCGCGACATACTGGCAATGTTCGCGCTTTCCGGTCATTCGGGTGTCTGACATCGCGAGCCTTAACGCAAGCCGTTTCTCGCGATACGTGGCGGCGTTTTCTCGACAGGTCGACGGTGGCTGTCTGACGCTGCAAACCGCTACCGTCAGTGGTCGAGCGGGAATTTTCTATTCCTCTGGAGTCCGAATTATATTCTCCGATTCCGAATTTGTCTGACACCGGAGGATATGACGTACAAGGGGTCGCGCTTGATCCCTGCCTCGCTTTTTTCGCCTATTTTCGTAGGTGTCTGACGCCGAAGGCACCGCGCCATACGGCGCGACACACCGCAACAACGTTGCATGGTGTGTGACGATCGTCTTAGGGGGCGGGGATTCCCCCGCTTCCTAAGACGATCGTAGCCAACTTCACTTCAGGTTTGGGCCGGTCGCGGAGCCTTCACCCGGTAAGGTACGGCGGTTCCCGCTCAAGG
oriT secondary structure
Predicted by RNAfold.
Download structure file
Reference
[1] Esther Fernández-González et al. (2016) A Functional oriT in the Ptw Plasmid of Burkholderia cenocepacia Can Be Recognized by the R388 Relaxase TrwC. Frontiers in molecular biosciences. 3:16. [PMID:27200362]
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 26157..52638
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| QU43_RS72610 (pBCA014) | 21408..21980 | - | 573 | WP_006487431 | PH domain-containing protein | - |
| QU43_RS76705 (pBCA015) | 21977..22144 | - | 168 | WP_006487400 | hypothetical protein | - |
| QU43_RS72615 (pBCA016) | 22141..23457 | - | 1317 | WP_006487416 | DUF1173 domain-containing protein | - |
| QU43_RS72620 (pBCA017) | 23454..24866 | - | 1413 | WP_006487358 | zeta toxin family protein | - |
| QU43_RS72625 (pBCA018) | 24877..25143 | - | 267 | WP_006487406 | hypothetical protein | - |
| QU43_RS72630 (pBCA019) | 25787..26167 | + | 381 | WP_020980049 | hypothetical protein | - |
| QU43_RS72635 (pBCA020) | 26157..29195 | - | 3039 | WP_006487373 | conjugal transfer protein TraG N-terminal domain-containing protein | traG |
| QU43_RS72640 (pBCA021) | 29205..30659 | - | 1455 | WP_006487442 | conjugal transfer protein TraH | traH |
| QU43_RS72645 (pBCA022) | 30652..31686 | - | 1035 | WP_006487369 | phage Gp37/Gp68 family protein | - |
| QU43_RS72650 (pBCA023) | 31683..32126 | - | 444 | WP_006487377 | HNH endonuclease | - |
| QU43_RS72655 (pBCA024) | 32123..32653 | - | 531 | WP_006487388 | hypothetical protein | - |
| QU43_RS72660 (pBCA025) | 32664..33497 | - | 834 | WP_006487410 | conjugal transfer protein TraF | traF |
| QU43_RS72665 (pBCA026) | 33502..33891 | - | 390 | WP_006493947 | hypothetical protein | - |
| QU43_RS72670 (pBCA026a) | 33888..34196 | - | 309 | WP_006493941 | hypothetical protein | - |
| QU43_RS76015 | 34193..34459 | - | 267 | WP_174900806 | hypothetical protein | - |
| QU43_RS72675 (pBCA027) | 34456..35700 | - | 1245 | WP_006487422 | conjugal transfer protein TraN | traN |
| QU43_RS72680 (pBCA028) | 35708..36829 | - | 1122 | WP_006487394 | hypothetical protein | - |
| QU43_RS72685 (pBCA029) | 36831..37208 | - | 378 | WP_006493944 | hypothetical protein | - |
| QU43_RS72690 (pBCA030) | 37210..37911 | - | 702 | WP_006487447 | type-F conjugative transfer system pilin assembly protein TrbC | trbC |
| QU43_RS72695 (pBCA031) | 37908..38918 | - | 1011 | WP_006487375 | conjugal transfer pilus assembly protein TraU | traU |
| QU43_RS72700 (pBCA032) | 38915..39553 | - | 639 | WP_006487379 | type-F conjugative transfer system protein TraW | traW |
| QU43_RS72705 (pBCA033) | 39540..40070 | - | 531 | WP_006487449 | signal peptidase I | - |
| QU43_RS72710 (pBCA034) | 40088..40306 | - | 219 | WP_006487354 | hypothetical protein | - |
| QU43_RS72715 (pBCA035) | 40284..41096 | - | 813 | WP_006487420 | GntR family transcriptional regulator | - |
| QU43_RS72720 (pBCA036) | 41110..41643 | - | 534 | WP_020980048 | phospholipase D family protein | - |
| QU43_RS72725 (pBCA037) | 41719..42084 | - | 366 | WP_006487376 | hypothetical protein | - |
| QU43_RS72730 (pBCA037a) | 42081..42473 | - | 393 | WP_006493936 | hypothetical protein | - |
| QU43_RS72735 | 42470..43084 | - | 615 | WP_006487383 | hypothetical protein | - |
| QU43_RS72740 | 43081..43821 | - | 741 | WP_006487402 | hypothetical protein | - |
| QU43_RS73025 | 43818..44333 | - | 516 | WP_006487408 | hypothetical protein | - |
| QU43_RS72750 (pBCA041) | 44330..46936 | - | 2607 | WP_006487362 | type IV secretion system protein TraC | virb4 |
| QU43_RS72755 (pBCA042) | 46939..47601 | - | 663 | WP_006487356 | type IV conjugative transfer system lipoprotein TraV | traV |
| QU43_RS72760 (pBCA043) | 47598..48392 | - | 795 | WP_006487398 | DsbC family protein | trbB |
| QU43_RS72765 (pBCA044) | 48385..49818 | - | 1434 | WP_006487384 | TraB/VirB10 family protein | traB |
| QU43_RS72770 (pBCA045) | 49811..50557 | - | 747 | WP_012493842 | type-F conjugative transfer system secretin TraK | traK |
| QU43_RS72775 (pBCA046) | 50496..51086 | - | 591 | WP_006487381 | TraE/TraK family type IV conjugative transfer system protein | traE |
| QU43_RS72780 (pBCA047) | 51093..51386 | - | 294 | WP_006487365 | type IV conjugative transfer system protein TraL | traL |
| QU43_RS72785 (pBCA048) | 51400..51792 | - | 393 | WP_006487426 | hypothetical protein | - |
| QU43_RS75355 (pBCA048a) | 51821..52165 | - | 345 | WP_006493942 | hypothetical protein | - |
| QU43_RS72790 (pBCA049) | 52162..52638 | - | 477 | WP_006487418 | lytic transglycosylase domain-containing protein | virB1 |
| QU43_RS72795 (pBCA050) | 53073..53345 | - | 273 | WP_006493934 | hypothetical protein | - |
| QU43_RS72800 (pBCA051) | 53467..54237 | - | 771 | WP_006487404 | 5-oxoprolinase subunit PxpA | - |
| QU43_RS75360 (pBCA052) | 54224..54460 | - | 237 | WP_006487429 | hypothetical protein | - |
| QU43_RS72805 (pBCA053) | 54457..55497 | - | 1041 | WP_006493948 | TRAP transporter substrate-binding protein DctP | - |
| QU43_RS72810 (pBCA054) | 55620..56480 | - | 861 | WP_227743146 | LuxR family transcriptional regulator | - |
Host bacterium
| ID | 1474 | GenBank | NC_011003 |
| Plasmid name | Ptw | Incompatibility group | - |
| Plasmid size | 92661 bp | Coordinate of oriT [Strand] | 65490..66190 [+] |
| Host baterium | Burkholderia cenocepacia J2315 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |