Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100992
Name   oriT_Plasmid_C_Kpneumoniae_MS6671 in_silico
Organism   Klebsiella pneumoniae strain MS6671
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NZ_LN824136 (1311..1785 [-], 475 nt)
oriT length   475 nt
IRs (inverted repeats)      IR1: 145..155, 161..171  (ATGTGTAATAC..GTATTACACAT)
  IR2: 208..218, 223..233  (GCATTTACGAT..ATCGTAATTGC)
  IR3: 335..344, 347..356  (CCTTTCATGC..GCATGAAAGG)
Location of nic site      242..243
Conserved sequence flanking the
  nic site    		  GGTGT|ATAGC
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 475 nt

>oriT_Plasmid_C_Kpneumoniae_MS6671
ACTCCGATACGTTGCCCTCCGAACGGTATTCAAGGTCGATTTTTTGCGCTTCGGTCACTCTTAAACTGATAGATGGCATAGGTTTCCTTTGTGTAATACCGATGTAATACATACGAATCTAGCATATATGCGGCTTAATTACACATGTGTAATACGTTGTGTATTACACATTAAAACACAAATTAGAATAATTTGTTTTGTTTTCAAGCATTTACGATGAAAATCGTAATTGCGTATGGTGTATAGCCGTTAAGGGATACCATACCACGCCTTTTTTAAGGGAGAAACCGGTGTTACGTGCAAGTGAATCGCTCAAAAAGCGTTCACATTCACACCTTTCATGCTTGCATGAAAGGAAACGGACGGGAATTAGACAAAAATAAGACACGAGGAGTAAGTTATTGAGACAAGAAAAGGACACAAATAAGACATTTTTTAGAAAAAAACATTGACTTGAGACTAGAAATGGACAATA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   1062 GenBank   WP_000932317
Name   RelaxaseofPlasmid_C_Kpneumoniae_MS6671 insolico UniProt ID   _
Length   969 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 969 a.a.        Molecular weight: 108425.51 Da        Isoelectric Point: 9.7925

>WP_000932317.1 MULTISPECIES: MobF family relaxase [Enterobacterales]
MLNITTITRQRVDKVVDYYADGADDYYAKDGNAMQWQGAGAEELGLTGEVEQKRFAKLLDGKVTDDISVM
RKTANSESKERLGYDLTFSAPKGVTLQALVNGDKRIIEAHDRAVTKAIEEAERLALGRFTVNKKTHVENT
NKLIVGKFRHETSRELDPDLHTHAFVMNLTKTSDGKWRSLTNDGIVNSLTLLGNVYKSELARELELAGYN
LRYDRNGTFDLAHFSQEQIAQFSQRSQQIEAELAKNGLSRETASHEQKNAAALKTRKSKGEVDRDVLYEG
WKNRAKELQIDFDSREWKGAANDDIARNTQPAALEKPIEYQADKVMTFAIRSLTERQSVITERELMDVAM
KHGYGRLTLDDIRAARERAITSGNLIKEEATYAAATTNKKQQNVAMTREQWVTELVKAGRNKDEARKLVD
KGITNGRLVQQKPRFTTQLAQQRERNILKMEREGRGKIQTPYTREFSEGWLASRTLKPEQLKAVMGIIHT
PNQFISVHGFAGTGKSYMTKSAADFLKEQGVHVTSLAPYGSQVKALQAEGLESRTLQSFLRASDKKIGPG
SVVFIDEAGVIPARQMEETMRVIRDAGARAVFLGDTKQTKAVEAGKPFEQLIKAGMETAYMKDIQRQKDP
ELLKAALNAAEGKIKASLTHVTSIVEEKNHDQRYRRIVGDYVAMPPTDRANALIITGTNDSRKKINAYIQ
AELGLKGQGINYPLLNRLDTTQAQRQHSKYYEKGAVIIPERDYSNGLKRGAVYTVLDTGPGNKLTVSDGS
GDTIAFSPARFSKLSVYSVEKTELAVGDQVRITRNDAHLDLANGDRFKVKGVQSGEVLLENEKGRLVKID
ANKPMYLGLAYASTVHSAQGLTCDKVFINMDTRSLTTAKDVYYVAVTRAKHEAVIYTDDEKKLDKAASRE
GFKTAALELEQLKRYAKEQQHDAREKGRDKTEPTQEKDNAKRKGKGHDKNNDERAFTSL

  Protein domains


Predicted by InterproScan.

AAA domain (AAA_30)

(475-649)

UvrD-like helicase C-terminal domain (UvrD_C_2)

(850-896)

TrwC relaxase (TrwC)

(14-287)


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 2047..19836

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MS6671_RS28815 (MS6671_pC0010) 109..819 - 711 WP_000686338 StbB family protein -
MS6671_RS28820 (MS6671_pC0020) 832..1275 - 444 WP_024249956 hypothetical protein -
MS6671_RS28825 (MS6671_pC0030) 1707..2054 + 348 WP_001139329 hypothetical protein -
MS6671_RS28830 (MS6671_pC0040) 2047..3624 + 1578 WP_001001035 type IV secretion system DNA-binding domain-containing protein virb4
MS6671_RS28835 (MS6671_pC0050) 3627..6536 + 2910 WP_000932317 MobF family relaxase -
MS6671_RS28840 (MS6671_pC0060) 6814..7008 - 195 WP_000529710 hypothetical protein -
MS6671_RS28845 (MS6671_pC0070) 7020..7502 - 483 WP_036896345 GNAT family protein -
MS6671_RS28850 (MS6671_pC0080) 7515..8069 - 555 WP_000616063 phospholipase D family protein -
MS6671_RS28855 (MS6671_pC0090) 8032..9063 - 1032 WP_000138004 P-type DNA transfer ATPase VirB11 virB11
MS6671_RS28860 (MS6671_pC0100) 9056..10210 - 1155 WP_001224296 type IV secretion system protein VirB10 virB10
MS6671_RS28865 (MS6671_pC0110) 10207..11094 - 888 WP_000744355 TrbG/VirB9 family P-type conjugative transfer protein virB9
MS6671_RS28870 (MS6671_pC0120) 11097..11813 - 717 WP_000914645 type IV secretion system protein virB8
MS6671_RS32850 (MS6671_pC0130) 11816..11986 - 171 WP_021536809 hypothetical protein -
MS6671_RS28875 (MS6671_pC0140) 12081..13124 - 1044 WP_000885742 type IV secretion system protein virB6
MS6671_RS28880 (MS6671_pC0150) 13143..13379 - 237 WP_000733633 EexN family lipoprotein -
MS6671_RS28885 (MS6671_pC0160) 13383..14090 - 708 WP_036896352 type IV secretion system protein virB5
MS6671_RS28890 (MS6671_pC0170) 14100..16730 - 2631 WP_000257330 VirB4 family type IV secretion/conjugal transfer ATPase virb4
MS6671_RS28895 (MS6671_pC0180) 16730..17044 - 315 WP_001053888 VirB3 family type IV secretion system protein virB3
MS6671_RS28900 (MS6671_pC0190) 17094..17384 - 291 WP_001090269 TrbC/VirB2 family protein virB2
MS6671_RS28905 (MS6671_pC0200) 17368..17685 - 318 WP_001267114 hypothetical protein -
MS6671_RS28910 (MS6671_pC0210) 17708..18448 - 741 WP_000815895 lytic transglycosylase domain-containing protein virB1
MS6671_RS31920 (MS6671_pC0220) 18511..18747 + 237 WP_000089855 H-NS histone family protein -
MS6671_RS28920 (MS6671_pC0230) 18759..19196 + 438 WP_000034608 hypothetical protein -
MS6671_RS28925 (MS6671_pC0240) 19202..19519 + 318 WP_001226231 TrbM/KikA/MpfK family conjugal transfer protein -
MS6671_RS28930 (MS6671_pC0250) 19522..19836 + 315 WP_000851068 hypothetical protein tfc15
MS6671_RS28935 (MS6671_pC0260) 19812..20537 + 726 WP_223307254 restriction endonuclease -
MS6671_RS33455 (MS6671_pC0270) 20538..20939 + 402 WP_023187076 Hha/YmoA family nucleoid-associated regulatory protein -
MS6671_RS28950 (MS6671_pC0280) 20999..21313 - 315 WP_001280992 hypothetical protein -
MS6671_RS31410 (MS6671_pC0290) 21339..21665 - 327 WP_000817647 endoribonuclease MazF -
MS6671_RS28955 (MS6671_pC0300) 21655..21951 - 297 WP_223307255 hypothetical protein -
MS6671_RS28960 (MS6671_pC0310) 21921..22463 - 543 WP_000711249 hypothetical protein -
MS6671_RS28965 (MS6671_pC0320) 22463..22708 - 246 WP_000195442 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
MS6671_RS28975 (MS6671_pC0340) 23170..23592 + 423 WP_000838908 hypothetical protein -
MS6671_RS28980 (MS6671_pC0350) 23592..24176 + 585 WP_000690619 recombinase family protein -

Host bacterium

ID   1450 GenBank   NZ_LN824136
Plasmid name   Plasmid_C_Kpneumoniae_MS6671 Incompatibility group   _
Plasmid size   34604 bp Coordinate of oriT [Strand]   1311..1785 [-]
Host baterium   Klebsiella pneumoniae strain MS6671

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -