Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 100985 |
| Name | oriT_pABWA45_3  |
| Organism | Escherichia coli strain ABWA45 |
| Sequence Completeness | intact |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP022157 (56257..56905 [-], 649 nt) |
| oriT length | 649 nt |
| IRs (inverted repeats) | IR1: 198..207, 211..220 (ATTAGGTGTT..AATACCTAAT) IR2:319..324, 330..335 (AAAAAA..TTTTTT) IR3: 393..402, 405..414 (GTATTCATGC..GCATGAATAC) |
| Location of nic site | 301..302 |
| Conserved sequence flanking the nic site |
GGTGT|ATAGC |
| Note | predicted by the oriTfinder |
oriT sequence
Download Length: 649 nt
>oriT_pABWA45_3
AGCGCCGCAGATAATCTGACCGATTACCTCCTGAAACCAGGTCTATATAGGCCAAAAGTTCATCTGATACTTTTGCGGTTATTATTGGCATTCAGTCCTCACATTGTGCATTTCTTAAACAAAAGATTGGGATCTAACAAGCTGAAATCTTAGTATTACCAAAGTAATAAAGCAAACTCATTATAAAACAATGAGTTATTAGGTGTTTTTAATACCTAATTATTACCGAATATTGACGCTATTTATTTTTTTATTTTTTAAATCAGTGTGATAGCGTGATTTATCGCGCTGCGTTAGGTGTATAGCAGGTTAAGGGATAAAAAATCATCTTTTTTGGTAGGAGCGATCTACGTAGGTTAAGGACTAACTGACTAAAAAGCGTTCAATATTCCGTATTCATGCTTGCATGAATACCAGTACAACACTATTACAACAAAAGTACATCAAAATTACATCAAAAGTACATCACTTGAAGGTTGACAGTACAACAGAATTACATCATTATCTGGTCATGAGGTAGCCAGTACAACAAAAGTACATCAAAAATACATCATAAATACATCAGAAATACATCAAAATTACATCATTCTAAATGAGGGTACTATGAAGCCCAAAAGTATCAGGGCGGCACTTCAGTTGATGTTGCCGG
AGCGCCGCAGATAATCTGACCGATTACCTCCTGAAACCAGGTCTATATAGGCCAAAAGTTCATCTGATACTTTTGCGGTTATTATTGGCATTCAGTCCTCACATTGTGCATTTCTTAAACAAAAGATTGGGATCTAACAAGCTGAAATCTTAGTATTACCAAAGTAATAAAGCAAACTCATTATAAAACAATGAGTTATTAGGTGTTTTTAATACCTAATTATTACCGAATATTGACGCTATTTATTTTTTTATTTTTTAAATCAGTGTGATAGCGTGATTTATCGCGCTGCGTTAGGTGTATAGCAGGTTAAGGGATAAAAAATCATCTTTTTTGGTAGGAGCGATCTACGTAGGTTAAGGACTAACTGACTAAAAAGCGTTCAATATTCCGTATTCATGCTTGCATGAATACCAGTACAACACTATTACAACAAAAGTACATCAAAATTACATCAAAAGTACATCACTTGAAGGTTGACAGTACAACAGAATTACATCATTATCTGGTCATGAGGTAGCCAGTACAACAAAAGTACATCAAAAATACATCATAAATACATCAGAAATACATCAAAATTACATCATTCTAAATGAGGGTACTATGAAGCCCAAAAGTATCAGGGCGGCACTTCAGTTGATGTTGCCGG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 1055 | GenBank | WP_011407157 |
| Name | Relaxase_pABWA45_3 |
UniProt ID | A0A1J5VQU8 |
| Length | 1078 a.a. | PDB ID | |
| Note | putative relaxase | ||
Relaxase protein sequence
Download Length: 1078 a.a. Molecular weight: 120179.41 Da Isoelectric Point: 6.5766
>WP_011407157.1 MULTISPECIES: MobF family relaxase [Enterobacterales]
MLDITTITRQNVTSVVGYYSDAKDDYYSKDSSFTSWQGTGAEALGLSGDVESARFKELLVGEIDTFTHMQ
RHVGDAKKERLGYDLTFSAPKGVSMQALIHGDKTIIEAHEKAVAAAVREAEKLAQARTTRQGKSVTQNTN
NLVVATFRHETSRALDPDLHTHAFVMNMTQREDGQWRALKNDELMRNKMHLGDVYKQELALELTKAGYEL
RYNSKNNTFDMAHFSDEQIRAFSRRSEQIEKGLAAMGLTRETADAQTKSRVSMATREKKTEHSREEIHQE
WASRAKTLGIDFDNREWQGHGKPLEADIARNMAPDFTSPEVKADRAIQFAVKSLSERDASFERQKLIQIA
NKQVLGHATIADVEKAYLKAVQKGAIIEGEARYQSTLKVGASVMAETLTRKEWIDSLTNSGMRADKARFA
VDDGIKNGRLKKTSHRVTTVEGIRLERSILTIESRGRGQMPRQLTAEIAGQLLSGKTLKKEQMRAVTEIV
TSKDRFVAAHGYAGTGKSYMTMAAKELLESQGLKVTALAPYGTQKKALEDDGLPARTVAAFLKAKDKKLD
EKSVVFIDEAGVIPARQMKQLMEVIEKHNARAVFLGDTSQTKAVEAGKPFEQLIKAGMQTSYMKDIQRQK
NEVLLEAVKYAAEGNAARALKNITGVNELKEEAPRLAQLADRYLSLSSEQQDATLIISGTNASRKTLNDY
IRGNLGLAGTGETFTLLDRVDSTQAERRDSRYFSKGQIIIPEQDYKNGMKRGESYQVLDTGPGNKLTVES
ISGEQIAFSPRTHTKLSVYQAVSAELAPGDKVMVTRNDKTLDVANGDRFTVKTVEGEKLTLEDKKGRTVE
LDKKQASYLSYAYATTVHKSQGLTCDRVLFNIDTKSLTTSKDVFYVGISRARHEVEIFTDDKKSLASSVS
RDSPKTTAAEIDRFFGLEARFKDIGRDTSLETRSAEKGLPEATGESMAFNQKPDEHNMTTGTDYQPVSNA
EDAFHLKQNPMDDSVGLRRHEAQQNDAELAHDYAAADDQQWSAQEYADYEHYAEASDYDFDSSIYDDYAM
PQTSQAEQSHTGKEHTHEHEHEEGGHEI
MLDITTITRQNVTSVVGYYSDAKDDYYSKDSSFTSWQGTGAEALGLSGDVESARFKELLVGEIDTFTHMQ
RHVGDAKKERLGYDLTFSAPKGVSMQALIHGDKTIIEAHEKAVAAAVREAEKLAQARTTRQGKSVTQNTN
NLVVATFRHETSRALDPDLHTHAFVMNMTQREDGQWRALKNDELMRNKMHLGDVYKQELALELTKAGYEL
RYNSKNNTFDMAHFSDEQIRAFSRRSEQIEKGLAAMGLTRETADAQTKSRVSMATREKKTEHSREEIHQE
WASRAKTLGIDFDNREWQGHGKPLEADIARNMAPDFTSPEVKADRAIQFAVKSLSERDASFERQKLIQIA
NKQVLGHATIADVEKAYLKAVQKGAIIEGEARYQSTLKVGASVMAETLTRKEWIDSLTNSGMRADKARFA
VDDGIKNGRLKKTSHRVTTVEGIRLERSILTIESRGRGQMPRQLTAEIAGQLLSGKTLKKEQMRAVTEIV
TSKDRFVAAHGYAGTGKSYMTMAAKELLESQGLKVTALAPYGTQKKALEDDGLPARTVAAFLKAKDKKLD
EKSVVFIDEAGVIPARQMKQLMEVIEKHNARAVFLGDTSQTKAVEAGKPFEQLIKAGMQTSYMKDIQRQK
NEVLLEAVKYAAEGNAARALKNITGVNELKEEAPRLAQLADRYLSLSSEQQDATLIISGTNASRKTLNDY
IRGNLGLAGTGETFTLLDRVDSTQAERRDSRYFSKGQIIIPEQDYKNGMKRGESYQVLDTGPGNKLTVES
ISGEQIAFSPRTHTKLSVYQAVSAELAPGDKVMVTRNDKTLDVANGDRFTVKTVEGEKLTLEDKKGRTVE
LDKKQASYLSYAYATTVHKSQGLTCDRVLFNIDTKSLTTSKDVFYVGISRARHEVEIFTDDKKSLASSVS
RDSPKTTAAEIDRFFGLEARFKDIGRDTSLETRSAEKGLPEATGESMAFNQKPDEHNMTTGTDYQPVSNA
EDAFHLKQNPMDDSVGLRRHEAQQNDAELAHDYAAADDQQWSAQEYADYEHYAEASDYDFDSSIYDDYAM
PQTSQAEQSHTGKEHTHEHEHEEGGHEI
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 1..16424
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| CES94_RS28355 (CES94_28355) | 1..1530 | + | 1530 | WP_000342688 | type IV secretion system DNA-binding domain-containing protein | virb4 |
| CES94_RS28360 (CES94_28360) | 1530..4766 | + | 3237 | WP_011407157 | MobF family relaxase | - |
| CES94_RS28365 (CES94_28365) | 4766..5392 | + | 627 | WP_000434930 | DUF6710 family protein | - |
| CES94_RS28370 (CES94_28370) | 5566..6099 | - | 534 | WP_000731968 | phospholipase D family protein | - |
| CES94_RS28375 (CES94_28375) | 6099..7094 | - | 996 | WP_000128596 | ATPase, T2SS/T4P/T4SS family | virB11 |
| CES94_RS28380 (CES94_28380) | 7136..8296 | - | 1161 | WP_000101711 | type IV secretion system protein VirB10 | virB10 |
| CES94_RS28385 (CES94_28385) | 8296..9180 | - | 885 | WP_000735067 | TrbG/VirB9 family P-type conjugative transfer protein | virB9 |
| CES94_RS28390 (CES94_28390) | 9191..9889 | - | 699 | WP_000646594 | type IV secretion system protein | virB8 |
| CES94_RS28395 (CES94_28395) | 9879..10025 | - | 147 | WP_001257173 | conjugal transfer protein TraN | - |
| CES94_RS28400 (CES94_28400) | 10108..11148 | - | 1041 | WP_000886022 | type IV secretion system protein | virB6 |
| CES94_RS28405 (CES94_28405) | 11164..11391 | - | 228 | WP_000734973 | IncN-type entry exclusion lipoprotein EexN | - |
| CES94_RS28410 (CES94_28410) | 11399..12112 | - | 714 | WP_000749362 | type IV secretion system protein | virB5 |
| CES94_RS28415 (CES94_28415) | 12130..14730 | - | 2601 | WP_001200711 | VirB4 family type IV secretion/conjugal transfer ATPase | virb4 |
| CES94_RS28420 (CES94_28420) | 14730..15047 | - | 318 | WP_000496058 | VirB3 family type IV secretion system protein | virB3 |
| CES94_RS28425 (CES94_28425) | 15097..15390 | - | 294 | WP_000209137 | TrbC/VirB2 family protein | virB2 |
| CES94_RS28430 (CES94_28430) | 15400..15681 | - | 282 | WP_000440698 | transcriptional repressor KorA | - |
| CES94_RS28435 (CES94_28435) | 15690..16424 | - | 735 | WP_000033783 | lytic transglycosylase domain-containing protein | virB1 |
| CES94_RS28440 (CES94_28440) | 16467..16838 | + | 372 | WP_011867773 | H-NS family nucleoid-associated regulatory protein | - |
| CES94_RS28445 (CES94_28445) | 16854..17231 | + | 378 | WP_000504251 | hypothetical protein | - |
| CES94_RS28450 (CES94_28450) | 17194..17508 | + | 315 | WP_000734776 | TrbM/KikA/MpfK family conjugal transfer protein | - |
| CES94_RS28455 (CES94_28455) | 17544..17855 | + | 312 | WP_001452736 | hypothetical protein | - |
| CES94_RS28460 (CES94_28460) | 18043..18552 | + | 510 | WP_000893479 | restriction endonuclease | - |
| CES94_RS28465 (CES94_28465) | 18557..18754 | + | 198 | WP_000844102 | hypothetical protein | - |
| CES94_RS28470 (CES94_28470) | 18816..19520 | - | 705 | WP_001067855 | IS6-like element IS26 family transposase | - |
| CES94_RS28480 (CES94_28480) | 19646..20401 | - | 756 | WP_012372818 | 16S rRNA (guanine(1405)-N(7))-methyltransferase RmtB1 | - |
Host bacterium
| ID | 1443 | GenBank | NZ_CP022157 |
| Plasmid name | pABWA45_3 | Incompatibility group | IncN |
| Plasmid size | 57232 bp | Coordinate of oriT [Strand] | 56257..56905 [-] |
| Host baterium | Escherichia coli strain ABWA45 |
Cargo genes
| Drug resistance gene | rmtB, blaTEM-1B |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |