Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100984
Name   oriT_pABWA45_2 in_silico
Organism   Escherichia coli strain ABWA45
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NZ_CP022156 (79709..80068 [-], 360 nt)
oriT length   360 nt
IRs (inverted repeats)      280..287, 289..296  (GCAAGATT..AATCTTGC)
Location of nic site      305..306
Conserved sequence flanking the
  nic site    		  GACGGGTGT|GG
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 360 nt

>oriT_pABWA45_2
GATTATCAAGAGTCACATGTGAATCGGCCATTTTTTTTATTAGAGTCAGTCTGGATTCACCAACTTCATACAGGCGAATCTGTTATACCGAAATTAAGGATTCGAATCCAGATTCGAATCCTTTAATTTGTCTTAATTTAGTGGCCTGATTCGCAGATGAATCTGGATTCGACTCCTTTACGATTCACTAAAGATTTGCATGTGAATCTAGTATCTATATCTGACATAGAACAACGATAACAATATTAACATAATATACTGATAATAAAGGATAATCATGCAAGATTAAATCTTGCGACGGGTGTGGGACTTTGATGTTAAGGAGCAGGCATACGGAAATATGTCAGCCAGGAAGAATTC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   1054 GenBank   WP_047719744
Name   TraI_pABWA45_2 insolico UniProt ID   _
Length   1765 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 1765 a.a.        Molecular weight: 192897.17 Da        Isoelectric Point: 5.0769

>WP_047719744.1 MULTISPECIES: conjugative transfer relaxase/helicase TraI [Enterobacteriaceae]
MLSISSIKGDAGYYSHEDNYYASGSLDSRWMGEGAEKLGLKGEVASADMDAVRQGRLPDGSDLSRMVDGV
NKHRSGYDLTFSAPKSVSVMALVGEDRRFIEAHNRAVAVVMKEVEHLVSARITQEGKTETVLTGSMVAAL
YNHDTSRDLDPQVHTHALVFNATFADEKWRSLASDTRMKTGFSENLYATKIALGNLYRSALREDIESMGF
ETVAAGKHGLWELKDVPVDIFSSRSQAIREAAGPDASAKSRDVAALDTRQAKAWADPDLLKADWRRRLTD
EKFDIGHYISQAQARVEITGSVVAGQGGMRAPGQPGIGSSGEAADELVQKAVSDTISALSDKKVQFTWSE
MLAGTVSRLPSAPGLFEQARAGIEAAIEGQRLIPLDREKGIFTSDIHLLNELSVHQLARTAVAEQTVLVF
PERAQERDIPAGDAVSVLSQDKSPVAILSGRGGAQTLRDRTEDVAMMARSQGREVMVIAADGRSGQFLSE
SPHLAGHVMLRSQMNADTVLPVQGTVIVDRAERLSLKETVLLQEKALSAGAQLIFMDTENRQGTGNALSV
LKEADIPQYRFYGTQLPEVRLISEADKRSRYGQLAQEYVRLSAEGRDVVAQVTGTREQQQLTEVIRDTRR
EAGELGREQVTLRVLEPVWLDSKTRHQRDNYRPGMVMEQWDAEKKTMTRHTIDRVAEATNSLVLQGEDGT
RLTLKVTQLDGSWSLYRSRTLEVSEGDRVRALGRELKGAIKAKEQFTLAGLENGAVRLRSGDRELRLPTE
RAVKLTHDYVEGTGAGTSASRTVLAAVGPRGLNKQALNALAQSGSDIRIYTPLAPEQAARKVESVSAVRL
ASDQVRQSTGEANLDTAIQASRDRLMSDAEQAVSLAIPRAQQGQVHLSEITLLSEAVKSGQPLADVRTEI
ARQVNSGELIQLDSVSGAGNRVLVPRVAYEMEKTIIRHIAEGKDAVQPLMALTPASVLAGLTAGQREATR
TVLENTDRFMAIQGYAGVGKTTQFRAVMGALNTLSESVRPQVIGLGPTHRAVHEMREAGVDARTLASFLS
ETRLAIQAGETPDFRNVLFLTDESSMVGNRDMAELYQLVTAGGGRMVSSGDTAQLQAISTGQPFRLVQQR
SAIDTVVMQEIVRQTPALRPAIESIIAGQVDTSLRLVDDVSPQQVPRQEGAWVPGNSVMEIRAPKKDQEQ
DTPAADEQTLTPEQLSLVRTDIIEAIRDDWMGRTPEAQLQTLVVAELNADRHAINDAIHAARHEKGDTGA
EERTFTVLEPLRVPDNALRAAETFAEYTGAVAMMNERYWTVAEVDTQDAVVTLRNADGESVLISPQQNTA
QDISLFTPRDLTISHGDRVRFTRSDNDRGYVANSLWEVAGFTDDGAIRFRQGEQEKIVDPQAMTEDRHID
LAYALTVYGVQGASERFAIALTGTEGGRKRMASLESTYVTLSRAKEHVQVYTDDLAGWSAGARHSNAGQT
AHDLLHQKSDHESDTGNRLLATASRLDKTALGRRVLAENGLEGETMARFIAAGRKYPTPYVALPAWTRHG
QEAGALLTEIRIEDDGMRVVLSDESRLRGGEDAQFAGLQASRNGQTLIADDAQTALRLAQENPESGVVIR
LHGEERLLNAARLTGGRITEPDEVARTVRSVAEVESAAKAEDPITLPPDEQQKLAEAQEKAARELAEQAR
QELLPAVPGEESTRPEPLLSADEERRLRDGTARGERELDEAIQEAVAEGRGIRQQVREQMLRTEREWVVN
VPEKDIELEKTLGGD

  Protein domains


Predicted by InterproScan.

DNA relaxase TraI 2B/2B-like domain (TraI_2B)

(644-723)

TrwC relaxase (TrwC)

(12-279)

DNA helicase TraI (TraI_C)

(1439-1556)

AAA domain (AAA_30)

(971-1158)

single-stranded DNA binding TraI N-terminal subdomain (ssDNA_TraI_N)

(586-638)


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 79178..105958

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CES94_RS28145 (CES94_28145) 74253..74606 + 354 WP_000872088 hypothetical protein -
CES94_RS28150 (CES94_28150) 74961..75335 + 375 WP_001271110 hypothetical protein -
CES94_RS28155 (CES94_28155) 76061..76465 + 405 WP_001096026 hypothetical protein -
CES94_RS28160 (CES94_28160) 76522..76869 + 348 WP_000550116 hypothetical protein -
CES94_RS28165 (CES94_28165) 76964..77110 + 147 WP_001159045 hypothetical protein -
CES94_RS28170 (CES94_28170) 77157..77990 + 834 WP_000080851 N-6 DNA methylase -
CES94_RS28180 (CES94_28180) 78581..79167 + 587 Protein_90 antirestriction protein -
CES94_RS28185 (CES94_28185) 79178..79633 - 456 WP_001232722 transglycosylase SLT domain-containing protein virB1
CES94_RS28190 (CES94_28190) 80102..80485 + 384 WP_001124390 conjugal transfer relaxosome DNA-binding protein TraM -
CES94_RS28195 (CES94_28195) 80786..81235 + 450 WP_001144045 helix-turn-helix domain-containing protein -
CES94_RS28200 (CES94_28200) 81441..81674 + 234 WP_000662610 TraY domain-containing protein -
CES94_RS28205 (CES94_28205) 81791..82150 + 360 WP_001054221 type IV conjugative transfer system pilin TraA -
CES94_RS28210 (CES94_28210) 82152..82457 + 306 WP_000398849 type IV conjugative transfer system protein TraL traL
CES94_RS28215 (CES94_28215) 82477..83040 + 564 WP_000406022 type IV conjugative transfer system protein TraE traE
CES94_RS28220 (CES94_28220) 83030..83770 + 741 WP_000794569 type-F conjugative transfer system secretin TraK traK
CES94_RS28225 (CES94_28225) 83757..85118 + 1362 WP_000024509 F-type conjugal transfer pilus assembly protein TraB traB
CES94_RS28230 (CES94_28230) 85229..85453 + 225 WP_000786061 TraR/DksA C4-type zinc finger protein -
CES94_RS28235 (CES94_28235) 85564..86178 + 615 WP_000724456 type IV conjugative transfer system lipoprotein TraV traV
CES94_RS28240 (CES94_28240) 86189..88780 + 2592 WP_000714154 type IV secretion system protein TraC virb4
CES94_RS28245 (CES94_28245) 88777..89241 + 465 WP_003830754 type-F conjugative transfer system protein TrbI -
CES94_RS28250 (CES94_28250) 89241..89876 + 636 WP_000715590 type-F conjugative transfer system protein TraW traW
CES94_RS28255 (CES94_28255) 89873..90868 + 996 WP_000833231 conjugal transfer pilus assembly protein TraU traU
CES94_RS28260 (CES94_28260) 90879..91238 + 360 WP_000961331 hypothetical protein -
CES94_RS28265 (CES94_28265) 91235..91849 + 615 WP_000832511 type-F conjugative transfer system pilin assembly protein TrbC trbC
CES94_RS28270 (CES94_28270) 91846..92361 + 516 Protein_108 conjugal transfer protein TraN -
CES94_RS28275 (CES94_28275) 92574..93251 + 678 WP_085451751 IS66-like element accessory protein TnpA -
CES94_RS28280 (CES94_28280) 93251..93598 + 348 WP_000624622 IS66 family insertion sequence element accessory protein TnpB -
CES94_RS28285 (CES94_28285) 93618..95189 + 1572 WP_000381395 IS66-like element ISCro1 family transposase -
CES94_RS28290 (CES94_28290) 95219..96406 + 1188 Protein_112 conjugal transfer protein TraN -
CES94_RS28295 (CES94_28295) 96403..97179 + 777 WP_001203361 type-F conjugative transfer system pilin assembly protein TraF traF
CES94_RS28300 (CES94_28300) 97205..97816 + 612 WP_000958503 type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB traF
CES94_RS28305 (CES94_28305) 97816..99177 + 1362 WP_001237460 conjugal transfer pilus assembly protein TraH traH
CES94_RS28310 (CES94_28310) 99179..102076 + 2898 WP_000257544 conjugal transfer mating-pair stabilization protein TraG traG
CES94_RS28315 (CES94_28315) 102088..102648 + 561 WP_000830402 hypothetical protein -
CES94_RS28320 (CES94_28320) 102824..103561 + 738 WP_000682257 conjugal transfer complement resistance protein TraT -
CES94_RS28325 (CES94_28325) 103712..105958 + 2247 WP_000056180 type IV conjugative transfer system coupling protein TraD virb4

Host bacterium

ID   1442 GenBank   NZ_CP022156
Plasmid name   pABWA45_2 Incompatibility group   IncFII
Plasmid size   113793 bp Coordinate of oriT [Strand]   79709..80068 [-]
Host baterium   Escherichia coli strain ABWA45

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   mercury resistance; copper resistance; arsenical resistance
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -