Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 100981 |
| Name | oriT_p704SK6_4  |
| Organism | Klebsiella pneumoniae isolate 704SK6 |
| Sequence Completeness | core |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP022147 (40150..40255 [+], 106 nt) |
| oriT length | 106 nt |
| IRs (inverted repeats) | 18..35, 41..58 (GTTTTTGGAGTACCGCCG..CGGCGGCCGTCCAAAAAC) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | predicted by the oriTfinder |
oriT sequence
Download Length: 106 nt
>oriT_p704SK6_4
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 1051 | GenBank | WP_004187323 |
| Name | Putativerelaxaseofp704SK6_4 |
UniProt ID | _ |
| Length | 659 a.a. | PDB ID | |
| Note | putative relaxase | ||
Relaxase protein sequence
Download Length: 659 a.a. Molecular weight: 75179.89 Da Isoelectric Point: 9.9948
>WP_004187323.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 43051..62356
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| CES89_RS29035 (CES89_29035) | 38135..38446 | + | 312 | WP_004187333 | hypothetical protein | - |
| CES89_RS29040 (CES89_29040) | 38579..39115 | + | 537 | WP_004187332 | hypothetical protein | - |
| CES89_RS29045 (CES89_29045) | 39617..40012 | - | 396 | WP_019725163 | hypothetical protein | - |
| CES89_RS30285 | 40047..40574 | + | 528 | WP_172740549 | plasmid mobilization protein MobA | - |
| CES89_RS29055 (CES89_29055) | 40561..42540 | + | 1980 | WP_004187323 | TraI/MobA(P) family conjugative relaxase | - |
| CES89_RS29060 (CES89_29060) | 42554..43054 | + | 501 | WP_004187320 | DotD/TraH family lipoprotein | - |
| CES89_RS29065 (CES89_29065) | 43051..43830 | + | 780 | WP_004187315 | type IV secretory system conjugative DNA transfer family protein | traI |
| CES89_RS29070 (CES89_29070) | 43841..45004 | + | 1164 | WP_004187313 | plasmid transfer ATPase TraJ | virB11 |
| CES89_RS29075 (CES89_29075) | 44994..45254 | + | 261 | WP_004187310 | IcmT/TraK family protein | traK |
| CES89_RS30290 | 45279..47378 | + | 2100 | WP_227522597 | DUF5710 domain-containing protein | - |
| CES89_RS30415 | 47375..47455 | + | 81 | Protein_67 | hypothetical protein | - |
| CES89_RS30300 | 47524..48222 | + | 699 | WP_227522610 | hypothetical protein | - |
| CES89_RS30305 | 48162..48593 | + | 432 | WP_227522598 | hypothetical protein | - |
| CES89_RS29090 (CES89_29090) | 49483..49788 | - | 306 | WP_227522599 | hypothetical protein | - |
| CES89_RS30315 | 49869..50156 | + | 288 | WP_227522611 | hypothetical protein | traM |
| CES89_RS29095 (CES89_29095) | 50128..50583 | + | 456 | WP_264755453 | DotI/IcmL family type IV secretion protein | traM |
| CES89_RS30320 (CES89_29100) | 50586..50864 | - | 279 | WP_227522601 | hypothetical protein | - |
| CES89_RS30420 (CES89_29110) | 51117..51749 | + | 633 | Protein_74 | DotH/IcmK family type IV secretion protein | - |
| CES89_RS30335 | 51761..52642 | + | 882 | WP_227522602 | conjugal transfer protein TraO | traO |
| CES89_RS30340 | 52728..53135 | + | 408 | WP_227522603 | hypothetical protein | traO |
| CES89_RS30425 | 53122..53250 | + | 129 | WP_264755454 | hypothetical protein | - |
| CES89_RS29120 (CES89_29120) | 53297..53827 | + | 531 | WP_264755456 | conjugal transfer protein TraP | traP |
| CES89_RS29125 (CES89_29125) | 53851..54381 | + | 531 | WP_004187478 | conjugal transfer protein TraQ | traQ |
| CES89_RS29130 (CES89_29130) | 54398..54787 | + | 390 | WP_004187479 | DUF6750 family protein | traR |
| CES89_RS29135 (CES89_29135) | 54832..55326 | + | 495 | WP_004187480 | hypothetical protein | - |
| CES89_RS30345 | 55323..56027 | + | 705 | WP_227522604 | hypothetical protein | traU |
| CES89_RS29140 (CES89_29140) | 55979..58372 | + | 2394 | WP_227522605 | conjugal transfer protein | traU |
| CES89_RS30430 | 58492..58668 | + | 177 | Protein_84 | hypothetical protein | - |
| CES89_RS29145 (CES89_29145) | 58698..59576 | + | 879 | WP_264755455 | conjugal transfer protein TraW | traW |
| CES89_RS29155 (CES89_29155) | 59573..62356 | + | 2784 | WP_227522606 | DotA/TraY family protein | traY |
| CES89_RS29160 (CES89_29160) | 62358..63011 | + | 654 | WP_015060005 | hypothetical protein | - |
| CES89_RS29165 (CES89_29165) | 63080..63322 | + | 243 | WP_023893611 | IncL/M type plasmid replication protein RepC | - |
Host bacterium
| ID | 1439 | GenBank | NZ_CP022147 |
| Plasmid name | p704SK6_4 | Incompatibility group | IncL/M |
| Plasmid size | 63605 bp | Coordinate of oriT [Strand] | 40150..40255 [+] |
| Host baterium | Klebsiella pneumoniae isolate 704SK6 |
Cargo genes
| Drug resistance gene | blaOXA-48 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrIIA8 |