Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 100979 |
| Name | oriT_p1605752AC2  |
| Organism | Klebsiella pneumoniae strain DHQP1605752_NV |
| Sequence Completeness | intact |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP022126 (63089..63639 [-], 551 nt) |
| oriT length | 551 nt |
| IRs (inverted repeats) | IR1: 62..70, 71..79 (AACCCTTTC..GACAGGGTT) IR2: 143..150, 154..161 (TGGCCTGC..GGAGGCCA) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | predicted by the oriTfinder |
oriT sequence
Download Length: 551 nt
>oriT_p1605752AC2
GAGCAGAGCTATGTGTGACAAGAAGTATAGAAATTACGAGGTAGCCATCATGGTCGATGTGAACCCTTTCGACAGGGTTATGAATGAATTGAAAAGTCGTGGCCGCAAGAACGCTCACATCCTGAGCATCCTCCAATTCGACTGGCCTGCATCGGAGGCCATCATCGAGAAGCTGAGCTGCTACATCACAGACGGGATTAAGGCTAATCAGGAGCCTGTGATTTACCCGATCATTGAAGAAGCTCTGCATCGCTACAGCCAGCTCGTGTTTCATGAGCAGAGAGAGAAATATGAAGACCCGGCCAGAATTGGGGCATTTCTGGAAACCCTGATCACCGAAACCTGCCGGGCGTTGGAAGTGCAAATTGTCGATAGTGGCGGTGATTCATGGTCTGTCGATTCAGGAGAGTCGTTCTCACTGTGGCTTTCTTCCCATCCAGGAGAACTATCCATTAACCCGCAGCCCCATGAGGATGAGACCTCTTTGCGTGGCTTGCTGTATGAGCTCATCACCTGTGAGAGCGTGAAAACTGTTTTAAGGAGAACCGACT
GAGCAGAGCTATGTGTGACAAGAAGTATAGAAATTACGAGGTAGCCATCATGGTCGATGTGAACCCTTTCGACAGGGTTATGAATGAATTGAAAAGTCGTGGCCGCAAGAACGCTCACATCCTGAGCATCCTCCAATTCGACTGGCCTGCATCGGAGGCCATCATCGAGAAGCTGAGCTGCTACATCACAGACGGGATTAAGGCTAATCAGGAGCCTGTGATTTACCCGATCATTGAAGAAGCTCTGCATCGCTACAGCCAGCTCGTGTTTCATGAGCAGAGAGAGAAATATGAAGACCCGGCCAGAATTGGGGCATTTCTGGAAACCCTGATCACCGAAACCTGCCGGGCGTTGGAAGTGCAAATTGTCGATAGTGGCGGTGATTCATGGTCTGTCGATTCAGGAGAGTCGTTCTCACTGTGGCTTTCTTCCCATCCAGGAGAACTATCCATTAACCCGCAGCCCCATGAGGATGAGACCTCTTTGCGTGGCTTGCTGTATGAGCTCATCACCTGTGAGAGCGTGAAAACTGTTTTAAGGAGAACCGACT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 1049 | GenBank | WP_001326173 |
| Name | TraI_p1605752AC2 |
UniProt ID | _ |
| Length | 990 a.a. | PDB ID | |
| Note | putative relaxase | ||
Relaxase protein sequence
Download Length: 990 a.a. Molecular weight: 109356.90 Da Isoelectric Point: 4.9395
>WP_001326173.1 MULTISPECIES: MobH family relaxase [Gammaproteobacteria]
MLKALNKLFGGRSGVIETAPSVRVLPLKDVEDEEIPRYPPFAKGLPVAPLDKILATQAELIEKVRNSLGF
TVDDFNRLVLPVIQRYAAFVHLLPASESHHHRGAGGLFRHGLEVAFWAAQASESVIFSIEGTPRERRDNE
PRWRLASCFSGLLHDVGKPLSDVSITDKDGSITWNPYSESLHDWAHRHEIDRYFIRWRDKRHKRHEQFSL
LAVDRIIPAETREFLSKSGPSIMEAMLEAISGTSVNQPVTKLMLRADQESVSRDLRQSRLDVDEFSYGVP
VERYVFDAIRRLVKTGKWKVNEPGAKVWHLNQGVFIAWKQLGDLYDLISHDKIPGIPRDPDTLADILIER
GFAVPNTVQEKGERAYYRYWEVLPEMLQEAAGSVKILMLRLESNDLVFTTEPPAAVAAEVVGDVEDAEIE
FVDPEEVDDDQEEDVSALNDDMLAAEQEAEKALAGLGFGDAMEMLKSTSDAVEEKPEQKDAGSTESSKPD
AGKKGKPQSKPGKAKPKSDTEKQPHKPEAKEDLSPQDIAKNAPPLANDNPLQALKDVGGGLGDIDFPFDA
FSASAETASTDATNSEIPDVAMPGKQEKQPKQDFVPQEQNSLQGDDFPMFGSSDEPPSWAIEPLPMLTDA
PEQTTPAPAMPPTDKPNLHEKDAKTLLVEMLAGYGEASALLEQAIMPVLEGKTTLGEVLCLMKGQAVILY
PDGARSLGAPSEVLSKLSHANAIVPDPIMPGRKVRDFSGVKAIVLAEQLSDAVVAAIKDAEASMGGYQDA
FELVSPPGLDASKNKSAPKQQSRKKAQQQKPEVNAGKPSPEQKAKGKDSQPQQKEKKVDVTSPVEEPQRQ
PVQEKQNVARLPKREVQPVAPEPKVEREKELGHVEVREREEPEVREFEPPKAKTNPKDINAEDFLPSGVT
PQKALQMLKDMIQKRSGRWLVTPVLEEDGCLVTSDKAFDMIAGENIGISKHILCGMLSRAQRRPLLKKRQ
GKLYLEVNET
MLKALNKLFGGRSGVIETAPSVRVLPLKDVEDEEIPRYPPFAKGLPVAPLDKILATQAELIEKVRNSLGF
TVDDFNRLVLPVIQRYAAFVHLLPASESHHHRGAGGLFRHGLEVAFWAAQASESVIFSIEGTPRERRDNE
PRWRLASCFSGLLHDVGKPLSDVSITDKDGSITWNPYSESLHDWAHRHEIDRYFIRWRDKRHKRHEQFSL
LAVDRIIPAETREFLSKSGPSIMEAMLEAISGTSVNQPVTKLMLRADQESVSRDLRQSRLDVDEFSYGVP
VERYVFDAIRRLVKTGKWKVNEPGAKVWHLNQGVFIAWKQLGDLYDLISHDKIPGIPRDPDTLADILIER
GFAVPNTVQEKGERAYYRYWEVLPEMLQEAAGSVKILMLRLESNDLVFTTEPPAAVAAEVVGDVEDAEIE
FVDPEEVDDDQEEDVSALNDDMLAAEQEAEKALAGLGFGDAMEMLKSTSDAVEEKPEQKDAGSTESSKPD
AGKKGKPQSKPGKAKPKSDTEKQPHKPEAKEDLSPQDIAKNAPPLANDNPLQALKDVGGGLGDIDFPFDA
FSASAETASTDATNSEIPDVAMPGKQEKQPKQDFVPQEQNSLQGDDFPMFGSSDEPPSWAIEPLPMLTDA
PEQTTPAPAMPPTDKPNLHEKDAKTLLVEMLAGYGEASALLEQAIMPVLEGKTTLGEVLCLMKGQAVILY
PDGARSLGAPSEVLSKLSHANAIVPDPIMPGRKVRDFSGVKAIVLAEQLSDAVVAAIKDAEASMGGYQDA
FELVSPPGLDASKNKSAPKQQSRKKAQQQKPEVNAGKPSPEQKAKGKDSQPQQKEKKVDVTSPVEEPQRQ
PVQEKQNVARLPKREVQPVAPEPKVEREKELGHVEVREREEPEVREFEPPKAKTNPKDINAEDFLPSGVT
PQKALQMLKDMIQKRSGRWLVTPVLEEDGCLVTSDKAFDMIAGENIGISKHILCGMLSRAQRRPLLKKRQ
GKLYLEVNET
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 56415..65505
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| CEV20_RS30565 (CEV20_01375) | 51440..51805 | - | 366 | Protein_53 | LuxR family transcriptional regulator | - |
| CEV20_RS01380 (CEV20_01380) | 52062..52379 | + | 318 | WP_000118520 | quaternary ammonium compound efflux SMR transporter SugE | - |
| CEV20_RS01385 (CEV20_01385) | 52376..52909 | - | 534 | WP_001221666 | lipocalin family protein | - |
| CEV20_RS01390 (CEV20_01390) | 53003..54148 | - | 1146 | WP_015058212 | class C beta-lactamase CMY-6 | - |
| CEV20_RS01395 (CEV20_01395) | 54472..55734 | - | 1263 | WP_000608644 | IS1380-like element ISEcp1 family transposase | - |
| CEV20_RS01400 (CEV20_01400) | 56019..56411 | - | 393 | WP_000479535 | TraA family conjugative transfer protein | - |
| CEV20_RS01405 (CEV20_01405) | 56415..56993 | - | 579 | WP_000793435 | type IV conjugative transfer system lipoprotein TraV | traV |
| CEV20_RS01410 (CEV20_01410) | 56990..58303 | - | 1314 | WP_024131605 | TraB/VirB10 family protein | traB |
| CEV20_RS01415 (CEV20_01415) | 58303..59220 | - | 918 | WP_000794249 | type-F conjugative transfer system secretin TraK | traK |
| CEV20_RS01420 (CEV20_01420) | 59204..59830 | - | 627 | WP_001049717 | TraE/TraK family type IV conjugative transfer system protein | traE |
| CEV20_RS01425 (CEV20_01425) | 59827..60108 | - | 282 | WP_000805625 | type IV conjugative transfer system protein TraL | traL |
| CEV20_RS01430 (CEV20_01430) | 60253..60618 | - | 366 | WP_001052530 | hypothetical protein | - |
| CEV20_RS29725 | 60630..60773 | - | 144 | WP_001275801 | hypothetical protein | - |
| CEV20_RS01435 (CEV20_01435) | 60954..61616 | - | 663 | WP_001231464 | hypothetical protein | - |
| CEV20_RS01440 (CEV20_01440) | 61616..61993 | - | 378 | WP_000869297 | hypothetical protein | - |
| CEV20_RS01445 (CEV20_01445) | 62003..62449 | - | 447 | WP_000122507 | hypothetical protein | - |
| CEV20_RS01450 (CEV20_01450) | 62459..63088 | - | 630 | WP_000743449 | DUF4400 domain-containing protein | tfc7 |
| CEV20_RS01455 (CEV20_01455) | 63045..63590 | - | 546 | WP_000228720 | hypothetical protein | - |
| CEV20_RS01460 (CEV20_01460) | 63640..65505 | - | 1866 | WP_000178857 | conjugative transfer system coupling protein TraD | virb4 |
| CEV20_RS01465 (CEV20_01465) | 65502..68474 | - | 2973 | WP_001326173 | MobH family relaxase | - |
| CEV20_RS01470 (CEV20_01470) | 68642..69259 | + | 618 | WP_001249395 | hypothetical protein | - |
| CEV20_RS01475 (CEV20_01475) | 69241..69474 | + | 234 | WP_001191890 | hypothetical protein | - |
Host bacterium
| ID | 1437 | GenBank | NZ_CP022126 |
| Plasmid name | p1605752AC2 | Incompatibility group | IncA/C2 |
| Plasmid size | 140133 bp | Coordinate of oriT [Strand] | 63089..63639 [-] |
| Host baterium | Klebsiella pneumoniae strain DHQP1605752_NV |
Cargo genes
| Drug resistance gene | blaNDM-1, rmtC, sul1, qacE, aac(6')-Ib, blaCMY-6 |
| Virulence gene | htpB |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |