Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100975
Name   oriT_unitig_4_pilon in_silico
Organism   Enterobacter cloacae complex sp. strain AR_0050
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NZ_CP021899 (68799..69436 [-], 638 nt)
oriT length   638 nt
IRs (inverted repeats)      IR1: 198..207, 211..220  (ATTGGGTGTT..AATACCTAAT)
 IR2:319..324, 330..335  (AAAAAA..TTTTTT)
  IR3: 393..402, 405..414  (GTATTCATGC..GCATGAATAC)
Location of nic site      301..302
Conserved sequence flanking the
  nic site    		  GGTGT|ATAGC
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 638 nt

>oriT_unitig_4_pilon
AGCGCCGCAGATAATCTGACCGATTACCTCCTGAAACCAGGTCTATATAGGCCAAAAGTTCATCTGATACTTTTGCGGTTATTATTGGCATTCAGTCCTCACATTGTGCATTTTTTAAACAAAAAATTGGGATCTAACAAGCTGAAATCTTAGTATTACCAAAGTAATAAAGCAAACTCATTATAAAACAATGAGTTATTGGGTGTTTTTAATACCTAATTATTACCGAATATTGTTGCTATTTATTTTTTTATCTTTTAAATCAGTATGATAGCGTGATTTATCGCGCTGCGTTAGGTGTATAGCAGGTTAAGGGATAAAAAATCATCTTTTTTGGTAGGGGCGATCTACGTAGGTTAAGGACTAACTGGCTAAAAAGCGTTCAATATTCCGTATTCATGCTTGCATGAATACCAGTACAACAAAAGTACATCAAAATTACATCAAAATTACATCACTTGAAGGTTGACAGTACAACAAAATTACATCATTCTTTGGTCATGAGGTAGCCAGTACAACAAAAGTACATCAAAAGTACATCAAAAGTACATCAAAATTACATCAAAATTACATCATTCTAAATGAGGGTACTATGAAGCCCAAAAGTATCAGGGCGGCACTTCAGTTGATGTTGCCGG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   1045 GenBank   WP_000884351
Name   TrwC_unitig_4_pilon insolico UniProt ID   _
Length   1076 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 1076 a.a.        Molecular weight: 119897.16 Da        Isoelectric Point: 6.6071

>WP_000884351.1 MULTISPECIES: MobF family relaxase [Enterobacterales]
MLDITTITRQNVTSVVGYYSDAKDDYYSKDSSFTSWQGTGAEALGLSGDVESARFKELLVGEIDTFTHMQ
RHVGDAKKERLGYDLTFSAPKGVSMQALIHGDKTIIEAHEKAVAAAVREAEKLAQARTTRQGKSVTQNTN
NLVVATFRHETSRALDPDLHTHAFVMNMTQREDGQWRALKNDELMRNKMHLGDVYKQELALELTKAGYEL
RYNSKNNTFDMAHFSDEQIRAFSRRSEQIEKGLAAMGLTRETADAQTKSRVSMATREKKTEHSREEIHQE
WASRAKTLGIDFDNREWQGHGKPLEADIARNMAPDFTSPEVKADRAIQFAVKSLSERDASFERQKLIQIA
NKQVLGHATIADVEKAYLKAVQKGAIIEGEARYQSTLKVGASVMAETLTRKEWIDSLTNSGMRADKARFA
VDDGIKNGRLKKTSHRVTTVEGIRLERSILTIESRGRGQMPRQLTAEIAGQLLAGKTLKKEQMRAVTEIV
TSKDRFVAAHGYAGTGKSYMTMAAKELLESQGLKVTALAPYGTQKKALEDDGLPARTVAAFLKAKDKKLD
EKSVVFIDEAGVIPARQMKQLMEVIEKHNARAVFLGDTSQTKAVEAGKPFEQLIKAGMQTSYMKDIQRQK
NEVLLEAVKYAAEGNAARALKNITGVNELKEEAPRLAQLADRYLSLSSEQQDATLIISGTNASRKTLNDY
IRGNLGLAGTGETFTLLDRVDSTQAERRDSRYFSKGQIIIPEQDYKNGMKRGESYQVLDTGPGNKLTVES
ISGEQIAFSPRTHTKLSVYQAVSAELAPGDKVMVTRNDKTLDVANGDRFTVKTVEGEKLTLEDKKGRTVE
LDKKQASYLSYAYATTVHKSQGLTCDRVLFNIDTKSLTTSKDVFYVGISRARHEVEIFTDDKKSLASSVS
RDSPKTTAAEIDRFFGLEARFKDIGRDTSLETRSAEKGLPEATGESMAFNQKPDEHNMTTGTDYQPVSNA
EDAFHLKQNPMDDSVGLRRHEAQQNDAELAHDYAAADDQQWSAQEYADYEHYAEASDYDFDSSIYDDYAM
PQTSQAEQSHTGKEHTHEHEEGGHEI

  Protein domains


Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C_2)

(851-895)

TrwC relaxase (TrwC)

(16-287)

AAA domain (AAA_30)

(477-653)


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 34638..44963

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM429_RS24295 (AM429_24215) 30505..31065 + 561 WP_001161490 recombinase family protein -
AM429_RS24300 (AM429_24220) 31069..34035 + 2967 WP_001138014 Tn3-like element TnAs1 family transposase -
AM429_RS24305 (AM429_24225) 34105..34638 - 534 WP_000731968 phospholipase D family protein -
AM429_RS24310 (AM429_24230) 34638..35633 - 996 WP_000128596 ATPase, T2SS/T4P/T4SS family virB11
AM429_RS24315 (AM429_24235) 35675..36835 - 1161 WP_000101712 type IV secretion system protein VirB10 virB10
AM429_RS24320 (AM429_24240) 36835..37719 - 885 WP_000735067 TrbG/VirB9 family P-type conjugative transfer protein virB9
AM429_RS24325 (AM429_24245) 37730..38428 - 699 WP_000646594 type IV secretion system protein virB8
AM429_RS24335 (AM429_24255) 38647..39687 - 1041 WP_000886022 type IV secretion system protein virB6
AM429_RS24340 (AM429_24260) 39703..39930 - 228 WP_000734973 IncN-type entry exclusion lipoprotein EexN -
AM429_RS24345 (AM429_24265) 39938..40651 - 714 WP_000749362 type IV secretion system protein virB5
AM429_RS24350 (AM429_24270) 40669..43137 - 2469 WP_225303560 VirB4 family type IV secretion/conjugal transfer ATPase virb4
AM429_RS24355 (AM429_24275) 43269..43586 - 318 WP_000496058 VirB3 family type IV secretion system protein virB3
AM429_RS24360 (AM429_24280) 43636..43929 - 294 WP_001749962 hypothetical protein virB2
AM429_RS24365 (AM429_24285) 43939..44220 - 282 WP_000440698 transcriptional repressor KorA -
AM429_RS24370 (AM429_24290) 44229..44963 - 735 WP_001749963 lytic transglycosylase domain-containing protein virB1
AM429_RS24375 (AM429_24295) 45072..45377 + 306 WP_000960954 H-NS family nucleoid-associated regulatory protein -
AM429_RS24380 (AM429_24300) 45393..45737 + 345 WP_001749964 hypothetical protein -
AM429_RS24385 (AM429_24305) 45734..46048 + 315 WP_001749965 TrbM/KikA/MpfK family conjugal transfer protein -
AM429_RS24390 (AM429_24310) 46084..46395 + 312 WP_001452736 hypothetical protein -
AM429_RS24395 (AM429_24315) 46445..47092 + 648 WP_015344958 restriction endonuclease -
AM429_RS24400 (AM429_24320) 47097..47303 + 207 WP_001749967 hypothetical protein -
AM429_RS24405 (AM429_24325) 47314..47575 - 262 Protein_48 IS1 family transposase -
AM429_RS24410 (AM429_24330) 47674..49107 + 1434 WP_001288432 DNA cytosine methyltransferase -

Host bacterium

ID   1433 GenBank   NZ_CP021899
Plasmid name   unitig_4_pilon Incompatibility group   IncN
Plasmid size   69635 bp Coordinate of oriT [Strand]   68799..69436 [-]
Host baterium   Enterobacter cloacae complex sp. strain AR_0050

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -