Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100965
Name   oriT_tig00000001_p1 in_silico
Organism   Klebsiella pneumoniae strain AR_0143
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NZ_CP021709 (1..458 [+], 458 nt)
oriT length   458 nt
IRs (inverted repeats)      50..57, 61..68  (TGGCCTGC..GGAGGCCA)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 458 nt

>oriT_tig00000001_p1
AAGTCGTGGCCGCAAGAACGCTCACATCCTGAGCATCCTCCAATTCGACTGGCCTGCATCGGAGGCCATCATCGAGAAGCTGAGCTGCTACATCACAGACGGGATTAAGGCTAATCAGGAGCCTGTGATTTACCCGATCATTGAAGAAGCTCTGCATCGCTACAGCCAGCTCGTGTTTCATGAGCAGAGAGAGAAATATGAAGACCCGGCCAGAATTGGGGCATTTCTGGAAACCCTGATCACCGAAACCTGCCGGGCGTTGGAAGTGCAAATTGTCGATAGTGGCGGTGATTCATGGTCTGTCGATTCAGGAGAGTCGTTCTCACTGTGGCTTTCTTCCCATCCAGGAGAACTATCCATTAACCCGCAGCCCCATGAGGATGAGACCTCTTTGCGTGGCTTGCTGTATGAGCTCATCACCTGTGAGAGCGTGAAAACTGTTTTAAGGAGAACCGACT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   1035 GenBank   WP_001326173
Name   TraI_tig00000001_p1 insolico UniProt ID   _
Length   990 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 990 a.a.        Molecular weight: 109356.90 Da        Isoelectric Point: 4.9395

>WP_001326173.1 MULTISPECIES: MobH family relaxase [Gammaproteobacteria]
MLKALNKLFGGRSGVIETAPSVRVLPLKDVEDEEIPRYPPFAKGLPVAPLDKILATQAELIEKVRNSLGF
TVDDFNRLVLPVIQRYAAFVHLLPASESHHHRGAGGLFRHGLEVAFWAAQASESVIFSIEGTPRERRDNE
PRWRLASCFSGLLHDVGKPLSDVSITDKDGSITWNPYSESLHDWAHRHEIDRYFIRWRDKRHKRHEQFSL
LAVDRIIPAETREFLSKSGPSIMEAMLEAISGTSVNQPVTKLMLRADQESVSRDLRQSRLDVDEFSYGVP
VERYVFDAIRRLVKTGKWKVNEPGAKVWHLNQGVFIAWKQLGDLYDLISHDKIPGIPRDPDTLADILIER
GFAVPNTVQEKGERAYYRYWEVLPEMLQEAAGSVKILMLRLESNDLVFTTEPPAAVAAEVVGDVEDAEIE
FVDPEEVDDDQEEDVSALNDDMLAAEQEAEKALAGLGFGDAMEMLKSTSDAVEEKPEQKDAGSTESSKPD
AGKKGKPQSKPGKAKPKSDTEKQPHKPEAKEDLSPQDIAKNAPPLANDNPLQALKDVGGGLGDIDFPFDA
FSASAETASTDATNSEIPDVAMPGKQEKQPKQDFVPQEQNSLQGDDFPMFGSSDEPPSWAIEPLPMLTDA
PEQTTPAPAMPPTDKPNLHEKDAKTLLVEMLAGYGEASALLEQAIMPVLEGKTTLGEVLCLMKGQAVILY
PDGARSLGAPSEVLSKLSHANAIVPDPIMPGRKVRDFSGVKAIVLAEQLSDAVVAAIKDAEASMGGYQDA
FELVSPPGLDASKNKSAPKQQSRKKAQQQKPEVNAGKPSPEQKAKGKDSQPQQKEKKVDVTSPVEEPQRQ
PVQEKQNVARLPKREVQPVAPEPKVEREKELGHVEVREREEPEVREFEPPKAKTNPKDINAEDFLPSGVT
PQKALQMLKDMIQKRSGRWLVTPVLEEDGCLVTSDKAFDMIAGENIGISKHILCGMLSRAQRRPLLKKRQ
GKLYLEVNET

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(51-357)


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 459..7132

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM390_RS27085 (AM390_26950) 459..1088 + 630 WP_000743449 DUF4400 domain-containing protein tfc7
AM390_RS27090 (AM390_26955) 1098..1544 + 447 WP_000122507 hypothetical protein -
AM390_RS27095 (AM390_26960) 1554..1931 + 378 WP_000869297 hypothetical protein -
AM390_RS27100 (AM390_26965) 1931..2593 + 663 WP_001231464 hypothetical protein -
AM390_RS31280 2774..2917 + 144 WP_001275801 hypothetical protein -
AM390_RS27105 (AM390_26970) 2929..3294 + 366 WP_001052530 hypothetical protein -
AM390_RS27110 (AM390_26975) 3439..3720 + 282 WP_000805625 type IV conjugative transfer system protein TraL traL
AM390_RS27115 (AM390_26980) 3717..4343 + 627 WP_001049717 TraE/TraK family type IV conjugative transfer system protein traE
AM390_RS27120 (AM390_26985) 4327..5244 + 918 WP_000794249 type-F conjugative transfer system secretin TraK traK
AM390_RS27125 (AM390_26990) 5244..6557 + 1314 WP_024131605 TraB/VirB10 family protein traB
AM390_RS27130 (AM390_26995) 6554..7132 + 579 WP_000793435 type IV conjugative transfer system lipoprotein TraV traV
AM390_RS27135 (AM390_27000) 7136..7528 + 393 WP_000479535 TraA family conjugative transfer protein -
AM390_RS27140 (AM390_27005) 7813..8985 + 1173 Protein_13 IS1380-like element ISEcp1 family transposase -
AM390_RS27145 (AM390_27010) 9091..10278 + 1188 WP_080399009 IS4 family transposase -
AM390_RS27150 (AM390_27015) 10733..11878 + 1146 WP_015056382 class C beta-lactamase CMY-4 -

Host bacterium

ID   1423 GenBank   NZ_CP021709
Plasmid name   tig00000001_p1 Incompatibility group   IncA/C2
Plasmid size   186758 bp Coordinate of oriT [Strand]   1..458 [+]
Host baterium   Klebsiella pneumoniae strain AR_0143

Cargo genes

Drug resistance gene   resistance to cetylpyridinium; quaternary ammonium compound resistance
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -