Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 100964 |
| Name | oriT_tig00000123  |
| Organism | Proteus mirabilis strain AR_0155 |
| Sequence Completeness | intact |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP021695 (60377..60927 [-], 551 nt) |
| oriT length | 551 nt |
| IRs (inverted repeats) | IR1: 62..70, 71..79 (AACCCTTTC..GACAGGGTT) IR2: 143..150, 154..161 (TGGCCTGC..GGAGGCCA) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | predicted by the oriTfinder |
oriT sequence
Download Length: 551 nt
>oriT_tig00000123
TGTAAAGAGGCTGTGAAAGAATAAGAGCATCAAGATTCCAGATAGATAGAGGGAAATTTGACAAATTCCAAAGATGGGTTAGCCTAGTGACAGAACTAGATTCCAGTATTGGAATAATCAGCTTTAAATTCCAGATAGATAGTTATGTGGATAGGAATTGGATAGGAATTGGGAGGGTATTGAGG
TGTAAAGAGGCTGTGAAAGAATAAGAGCATCAAGATTCCAGATAGATAGAGGGAAATTTGACAAATTCCAAAGATGGGTTAGCCTAGTGACAGAACTAGATTCCAGTATTGGAATAATCAGCTTTAAATTCCAGATAGATAGTTATGTGGATAGGAATTGGATAGGAATTGGGAGGGTATTGAGG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 1034 | GenBank | WP_001337757 |
| Name | TraI_tig00000123 |
UniProt ID | _ |
| Length | 992 a.a. | PDB ID | |
| Note | putative relaxase | ||
Relaxase protein sequence
Download Length: 992 a.a. Molecular weight: 109581.85 Da Isoelectric Point: 4.8956
>WP_001337757.1 MULTISPECIES: MobH family relaxase [Gammaproteobacteria]
MLKALNKLFGGRSGVIETAPSARVLPLKDVEDEEIPRYPPFAKGLPVAPLDKILATQAELIEKVRNSLGF
TVDDFNRLVLPVIQRYAAFVHLLPASESHHHRGAGGLFRHGLEVAFWAAQASESVIFSIEGTPRERRDNE
PRWRLASCFSGLLHDVGKPLSDVSITDKDGSITWNPYSESLHDWAHRHEIDRYFIRWRDKRHKRHEQFSL
LAVDRIIPAETREFLSKSGPSIMEAMLEAISGTSVNQPVTKLMLRADQESVSRDLRQSRLDVDEFSYGVP
VERYVFDAIRRLVKTGKWKVNEPGAKVWHLNQGVFIAWKQLGDLYDLISHDKIPGIPRDPDTLADILIER
GFAVPNTVQEKGERAYYRYWEVLPEMLQEAAGSVKILMLRLESNDLVFTTEPPAAVAAEVVGDVEDAEIE
FVDPEEADDGDDQEEGEAALNDDMLAAEQEAEKALAGLGFGDAMEMLKSTSDAVEEKPEQKDAGPTESSK
PDAGKKGKPQSKPGKAKPKSDTEKQPHKPEAKEDLSPQDIAKNAPPLANDNPLQALKDVGGGLGDIDFPF
DAFNASAETTSTDATNSEIPDVAMPGKQEEQPKQDFVPQEQNSLQGDDFPMFGGSDEPPSWAIEPLPMLT
DAPEQPTHTPEMPHTDNVNQHEKDAKTLLVEMLSGYGEASALLEQAIMPVLEGKTTLGEVLCLMKGQAVI
LYPDGARSLGAPSEVLSKLSHANAIVPDPIMPGRKVRDFSGVKAIVLAEQLSDAVVAAIKDAEASMGGYQ
DAFELVSPPGLDASKNKSAPKQQSRKKAQQQKPEVNAGKASPEQKAKGKDSQPQPKEKKVDVTSPVEEQQ
RKPVQEKQNVARLPKREAQPVAPEPKVEREKELGHVEVREREDPEVREFEPPKAKTNPKDINAEDFLPSG
VTPQKALQMLKDMIQKRSGRWLVTPVLEEDGCLVTSDKAFDMIAGENIGISKHILCGMLSRAQRRPLLKK
RQGKLYLEVNET
MLKALNKLFGGRSGVIETAPSARVLPLKDVEDEEIPRYPPFAKGLPVAPLDKILATQAELIEKVRNSLGF
TVDDFNRLVLPVIQRYAAFVHLLPASESHHHRGAGGLFRHGLEVAFWAAQASESVIFSIEGTPRERRDNE
PRWRLASCFSGLLHDVGKPLSDVSITDKDGSITWNPYSESLHDWAHRHEIDRYFIRWRDKRHKRHEQFSL
LAVDRIIPAETREFLSKSGPSIMEAMLEAISGTSVNQPVTKLMLRADQESVSRDLRQSRLDVDEFSYGVP
VERYVFDAIRRLVKTGKWKVNEPGAKVWHLNQGVFIAWKQLGDLYDLISHDKIPGIPRDPDTLADILIER
GFAVPNTVQEKGERAYYRYWEVLPEMLQEAAGSVKILMLRLESNDLVFTTEPPAAVAAEVVGDVEDAEIE
FVDPEEADDGDDQEEGEAALNDDMLAAEQEAEKALAGLGFGDAMEMLKSTSDAVEEKPEQKDAGPTESSK
PDAGKKGKPQSKPGKAKPKSDTEKQPHKPEAKEDLSPQDIAKNAPPLANDNPLQALKDVGGGLGDIDFPF
DAFNASAETTSTDATNSEIPDVAMPGKQEEQPKQDFVPQEQNSLQGDDFPMFGGSDEPPSWAIEPLPMLT
DAPEQPTHTPEMPHTDNVNQHEKDAKTLLVEMLSGYGEASALLEQAIMPVLEGKTTLGEVLCLMKGQAVI
LYPDGARSLGAPSEVLSKLSHANAIVPDPIMPGRKVRDFSGVKAIVLAEQLSDAVVAAIKDAEASMGGYQ
DAFELVSPPGLDASKNKSAPKQQSRKKAQQQKPEVNAGKASPEQKAKGKDSQPQPKEKKVDVTSPVEEQQ
RKPVQEKQNVARLPKREAQPVAPEPKVEREKELGHVEVREREDPEVREFEPPKAKTNPKDINAEDFLPSG
VTPQKALQMLKDMIQKRSGRWLVTPVLEEDGCLVTSDKAFDMIAGENIGISKHILCGMLSRAQRRPLLKK
RQGKLYLEVNET
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 37602..62793
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| AM402_RS20830 (AM402_20865) | 32729..33637 | - | 909 | WP_000739139 | hypothetical protein | - |
| AM402_RS20835 (AM402_20870) | 33648..34616 | - | 969 | WP_000085162 | AAA family ATPase | - |
| AM402_RS20840 (AM402_20875) | 34836..35021 | - | 186 | WP_001186917 | hypothetical protein | - |
| AM402_RS20845 (AM402_20880) | 35325..35645 | - | 321 | WP_000547566 | hypothetical protein | - |
| AM402_RS20850 (AM402_20885) | 35939..36580 | + | 642 | WP_000796664 | hypothetical protein | - |
| AM402_RS20855 (AM402_20890) | 36703..37563 | + | 861 | WP_000709517 | hypothetical protein | - |
| AM402_RS20860 (AM402_20895) | 37602..40412 | - | 2811 | WP_001909286 | conjugal transfer mating pair stabilization protein TraN | traN |
| AM402_RS20865 (AM402_20900) | 40516..41520 | - | 1005 | WP_227656952 | TraU family protein | traU |
| AM402_RS20870 (AM402_20905) | 41520..42191 | - | 672 | WP_001337754 | EAL domain-containing protein | - |
| AM402_RS20875 (AM402_20910) | 42188..43453 | - | 1266 | WP_000621288 | TrbC family F-type conjugative pilus assembly protein | traW |
| AM402_RS20880 (AM402_20915) | 43416..43946 | - | 531 | WP_001010738 | S26 family signal peptidase | - |
| AM402_RS20885 (AM402_20920) | 43943..44260 | - | 318 | WP_000351984 | hypothetical protein | - |
| AM402_RS20890 (AM402_20925) | 44275..46722 | - | 2448 | WP_000637386 | type IV secretion system protein TraC | virb4 |
| AM402_RS20895 (AM402_20930) | 46719..47426 | - | 708 | WP_001259347 | DsbC family protein | - |
| AM402_RS20900 (AM402_20935) | 47575..53106 | - | 5532 | WP_000606833 | Ig-like domain-containing protein | - |
| AM402_RS20905 (AM402_20940) | 53307..53699 | - | 393 | WP_000479535 | TraA family conjugative transfer protein | - |
| AM402_RS20910 (AM402_20945) | 53703..54281 | - | 579 | WP_000793435 | type IV conjugative transfer system lipoprotein TraV | traV |
| AM402_RS20915 (AM402_20950) | 54278..55591 | - | 1314 | WP_024131605 | TraB/VirB10 family protein | traB |
| AM402_RS20920 (AM402_20955) | 55591..56508 | - | 918 | WP_000794249 | type-F conjugative transfer system secretin TraK | traK |
| AM402_RS20925 (AM402_20960) | 56492..57118 | - | 627 | WP_001049716 | TraE/TraK family type IV conjugative transfer system protein | traE |
| AM402_RS20930 (AM402_20965) | 57115..57396 | - | 282 | WP_000805625 | type IV conjugative transfer system protein TraL | traL |
| AM402_RS20935 (AM402_20970) | 57541..57906 | - | 366 | WP_001052531 | hypothetical protein | - |
| AM402_RS22400 | 57918..58061 | - | 144 | WP_001275801 | hypothetical protein | - |
| AM402_RS20940 (AM402_20975) | 58242..58904 | - | 663 | WP_001231463 | hypothetical protein | - |
| AM402_RS20945 (AM402_20980) | 58904..59281 | - | 378 | WP_000869261 | hypothetical protein | - |
| AM402_RS22405 | 59523..59699 | - | 177 | WP_169342285 | hypothetical protein | - |
| AM402_RS20955 (AM402_20990) | 59747..60376 | - | 630 | WP_000743450 | DUF4400 domain-containing protein | tfc7 |
| AM402_RS20960 (AM402_20995) | 60333..60878 | - | 546 | WP_000228720 | hypothetical protein | - |
| AM402_RS20965 (AM402_21000) | 60928..62793 | - | 1866 | WP_000178856 | conjugative transfer system coupling protein TraD | virb4 |
| AM402_RS20970 (AM402_21005) | 62790..65768 | - | 2979 | WP_001337757 | MobH family relaxase | - |
| AM402_RS20975 (AM402_21010) | 65936..66553 | + | 618 | WP_001249396 | hypothetical protein | - |
| AM402_RS20980 (AM402_21015) | 66535..66768 | + | 234 | WP_001191892 | hypothetical protein | - |
Region 2: 98372..108865
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| AM402_RS21165 (AM402_21200) | 93661..94167 | + | 507 | WP_000140066 | arsenate reductase ArsC | - |
| AM402_RS21170 (AM402_21205) | 94164..95231 | + | 1068 | WP_001272375 | ACR3 family arsenite efflux transporter | - |
| AM402_RS21175 (AM402_21210) | 95346..95549 | + | 204 | WP_000131871 | hypothetical protein | - |
| AM402_RS22180 | 95586..95651 | - | 66 | Protein_113 | type I toxin-antitoxin system ptaRNA1 family toxin | - |
| AM402_RS21185 (AM402_21220) | 95737..96762 | + | 1026 | WP_021545039 | IS21-like element ISEc57 family transposase | - |
| AM402_RS21190 (AM402_21225) | 96841..97931 | + | 1091 | WP_085940413 | IS4-like element ISAba1 family transposase | - |
| AM402_RS21195 (AM402_21230) | 97984..98349 | - | 366 | Protein_116 | single-stranded DNA-binding protein | - |
| AM402_RS21200 (AM402_21235) | 98372..99586 | - | 1215 | WP_157673317 | TrbI/VirB10 family protein | virB10 |
| AM402_RS21205 (AM402_21240) | 99579..100025 | - | 447 | WP_087726749 | hypothetical protein | - |
| AM402_RS21210 (AM402_21245) | 100029..100943 | - | 915 | WP_087726750 | P-type conjugative transfer protein TrbG | virB9 |
| AM402_RS21215 (AM402_21250) | 100955..101599 | - | 645 | WP_071434332 | type IV secretion system protein | virB8 |
| AM402_RS21220 (AM402_21255) | 101596..103203 | - | 1608 | WP_087726751 | P-type conjugative transfer protein TrbL | virB6 |
| AM402_RS21225 (AM402_21260) | 103222..103464 | - | 243 | WP_087726752 | hypothetical protein | - |
| AM402_RS21230 (AM402_21265) | 103467..104195 | - | 729 | WP_087726753 | hypothetical protein | - |
| AM402_RS21235 (AM402_21270) | 104192..106627 | - | 2436 | WP_232467378 | hypothetical protein | virb4 |
| AM402_RS21240 (AM402_21275) | 106690..107010 | - | 321 | WP_032300801 | conjugal transfer protein TrbD | virB3 |
| AM402_RS21245 (AM402_21280) | 107016..107375 | - | 360 | WP_087726754 | TrbC/VirB2 family protein | virB2 |
| AM402_RS21250 (AM402_21285) | 107385..108329 | - | 945 | WP_087726755 | P-type conjugative transfer ATPase TrbB | virB11 |
| AM402_RS21255 (AM402_21290) | 108326..108865 | - | 540 | WP_135180852 | lytic transglycosylase domain-containing protein | virB1 |
| AM402_RS21260 (AM402_21295) | 108956..109489 | - | 534 | WP_157673318 | S26 family signal peptidase | - |
| AM402_RS21265 (AM402_21300) | 109595..109915 | + | 321 | WP_029403601 | hypothetical protein | - |
| AM402_RS21270 (AM402_21305) | 109972..110217 | + | 246 | WP_029403600 | helix-turn-helix transcriptional regulator | - |
| AM402_RS21275 (AM402_21310) | 110247..111251 | - | 1005 | WP_082242808 | hypothetical protein | - |
| AM402_RS21280 (AM402_21315) | 111238..113352 | - | 2115 | WP_087726757 | MFS transporter | - |
Host bacterium
| ID | 1422 | GenBank | NZ_CP021695 |
| Plasmid name | tig00000123 | Incompatibility group | IncA/C2 |
| Plasmid size | 214441 bp | Coordinate of oriT [Strand] | 60377..60927 [-] |
| Host baterium | Proteus mirabilis strain AR_0155 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | mercury resistance |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |