Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100960
Name   oriT_tig00000137 in_silico
Organism   Proteus mirabilis strain AR_0159
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NZ_CP021551 (14451..15001 [-], 551 nt)
oriT length   551 nt
IRs (inverted repeats)      IR1: 62..70, 71..79  (AACCCTTTC..GACAGGGTT)
  IR2: 143..150, 154..161  (TGGCCTGC..GGAGGCCA)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 551 nt

>oriT_tig00000137
GAGCAGAGCTATGTGTGACAAGAAGTATAGAAATTACGAGGTAGCCATCATGGTCGATGTGAACCCTTTCGACAGGGTTATGAATGAATTGAAAAGTCGTGGCCGCAAGAACGCTCACATCCTGAGCATCCTCCAATTCGACTGGCCTGCATCGGAGGCCATCATCGAGAAGCTGAGCTGCTACATCACAGACGGGATTAAGGCTAATCAGGAGCCTGTGATTTACCCGATCATTGAAGAAGCTCTGCATCGCTACAGCCAGCTCGTGTTTCATGAGCAGAGAGAGAAATATGAAGACCCGGCCAGAATTGGGGCATTTCTGGAAACCCTGATCACCGAAACCTGCCGGGCGTTGGAAGTGCAAATTGTCGATAGTGGCGGTGATTCATGGTCTGTCGATTCAGGAGAGTCGTTCTCACTGTGGCTTTCTTCCCATCCAGGAGAACTATCCATTAACCCGCAGCCCCATGAGGATGAGACCTCTTTGCGTGGCTTGCTGTATGAGCTCATCACCTGTGAGAGCGTGAAAACTGTTTTAAGGAGAACCGACT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   1030 GenBank   WP_001326173
Name   TraI_tig00000137 insolico UniProt ID   _
Length   990 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 990 a.a.        Molecular weight: 109356.90 Da        Isoelectric Point: 4.9395

>WP_001326173.1 MULTISPECIES: MobH family relaxase [Gammaproteobacteria]
MLKALNKLFGGRSGVIETAPSVRVLPLKDVEDEEIPRYPPFAKGLPVAPLDKILATQAELIEKVRNSLGF
TVDDFNRLVLPVIQRYAAFVHLLPASESHHHRGAGGLFRHGLEVAFWAAQASESVIFSIEGTPRERRDNE
PRWRLASCFSGLLHDVGKPLSDVSITDKDGSITWNPYSESLHDWAHRHEIDRYFIRWRDKRHKRHEQFSL
LAVDRIIPAETREFLSKSGPSIMEAMLEAISGTSVNQPVTKLMLRADQESVSRDLRQSRLDVDEFSYGVP
VERYVFDAIRRLVKTGKWKVNEPGAKVWHLNQGVFIAWKQLGDLYDLISHDKIPGIPRDPDTLADILIER
GFAVPNTVQEKGERAYYRYWEVLPEMLQEAAGSVKILMLRLESNDLVFTTEPPAAVAAEVVGDVEDAEIE
FVDPEEVDDDQEEDVSALNDDMLAAEQEAEKALAGLGFGDAMEMLKSTSDAVEEKPEQKDAGSTESSKPD
AGKKGKPQSKPGKAKPKSDTEKQPHKPEAKEDLSPQDIAKNAPPLANDNPLQALKDVGGGLGDIDFPFDA
FSASAETASTDATNSEIPDVAMPGKQEKQPKQDFVPQEQNSLQGDDFPMFGSSDEPPSWAIEPLPMLTDA
PEQTTPAPAMPPTDKPNLHEKDAKTLLVEMLAGYGEASALLEQAIMPVLEGKTTLGEVLCLMKGQAVILY
PDGARSLGAPSEVLSKLSHANAIVPDPIMPGRKVRDFSGVKAIVLAEQLSDAVVAAIKDAEASMGGYQDA
FELVSPPGLDASKNKSAPKQQSRKKAQQQKPEVNAGKPSPEQKAKGKDSQPQQKEKKVDVTSPVEEPQRQ
PVQEKQNVARLPKREVQPVAPEPKVEREKELGHVEVREREEPEVREFEPPKAKTNPKDINAEDFLPSGVT
PQKALQMLKDMIQKRSGRWLVTPVLEEDGCLVTSDKAFDMIAGENIGISKHILCGMLSRAQRRPLLKKRQ
GKLYLEVNET

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(51-357)


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 7777..16867

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM405_RS18750 (AM405_18760) 7381..7773 - 393 WP_000479535 TraA family conjugative transfer protein -
AM405_RS18755 (AM405_18765) 7777..8355 - 579 WP_000793435 type IV conjugative transfer system lipoprotein TraV traV
AM405_RS18760 (AM405_18770) 8352..9665 - 1314 WP_024131605 TraB/VirB10 family protein traB
AM405_RS18765 (AM405_18775) 9665..10582 - 918 WP_000794249 type-F conjugative transfer system secretin TraK traK
AM405_RS18770 (AM405_18780) 10566..11192 - 627 WP_001049717 TraE/TraK family type IV conjugative transfer system protein traE
AM405_RS18775 (AM405_18785) 11189..11470 - 282 WP_000805625 type IV conjugative transfer system protein TraL traL
AM405_RS18780 (AM405_18790) 11615..11980 - 366 WP_001052530 hypothetical protein -
AM405_RS20000 11992..12135 - 144 WP_001275801 hypothetical protein -
AM405_RS18785 (AM405_18795) 12316..12978 - 663 WP_001231464 hypothetical protein -
AM405_RS18790 (AM405_18800) 12978..13355 - 378 WP_000869297 hypothetical protein -
AM405_RS20005 13597..13773 - 177 WP_169342285 hypothetical protein -
AM405_RS18800 (AM405_18810) 13821..14450 - 630 WP_000743449 DUF4400 domain-containing protein tfc7
AM405_RS18805 (AM405_18815) 14407..14952 - 546 WP_000228720 hypothetical protein -
AM405_RS18810 (AM405_18820) 15002..16867 - 1866 WP_000178857 conjugative transfer system coupling protein TraD virb4
AM405_RS18815 (AM405_18825) 16864..19836 - 2973 WP_001326173 MobH family relaxase -
AM405_RS18820 (AM405_18830) 20004..20621 + 618 WP_001249395 hypothetical protein -
AM405_RS18825 (AM405_18835) 20603..20836 + 234 WP_001191890 hypothetical protein -

Host bacterium

ID   1418 GenBank   NZ_CP021551
Plasmid name   tig00000137 Incompatibility group   IncA/C2
Plasmid size   161830 bp Coordinate of oriT [Strand]   14451..15001 [-]
Host baterium   Proteus mirabilis strain AR_0159

Cargo genes

Drug resistance gene   mph(E), msr(E), armA, sul1, blaNDM-1, qacE, aadA2, dfrA12
Virulence gene   -
Metal resistance gene   merR
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -