Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 100956 |
| Name | oriT_p2474-3  |
| Organism | Escherichia coli strain strain Z247 |
| Sequence Completeness | intact |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP021208 (58494..58603 [-], 110 nt) |
| oriT length | 110 nt |
| IRs (inverted repeats) | IR1: 24..31, 35..42 (GTCGGGGC..GCCCTGAC) IR2: 49..65, 72..88 (GCAATTGTAATAGCGTC..GACGGTATTACAATTGC) |
| Location of nic site | 96..97 |
| Conserved sequence flanking the nic site |
CATCCTG|T |
| Note | predicted by the oriTfinder |
oriT sequence
Download Length: 110 nt
>oriT_p2474-3
TCACTTCAGGCTCCTTACGGGGTGTCGGGGCGAAGCCCTGACCAGATGGCAATTGTAATAGCGTCGCGTGTGACGGTATTACAATTGCACATCCTGTCCCGTTTTTCAGG
TCACTTCAGGCTCCTTACGGGGTGTCGGGGCGAAGCCCTGACCAGATGGCAATTGTAATAGCGTCGCGTGTGACGGTATTACAATTGCACATCCTGTCCCGTTTTTCAGG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 1026 | GenBank | WP_086353228 |
| Name | Mob_p2474-3 |
UniProt ID | _ |
| Length | 687 a.a. | PDB ID | |
| Note | putative relaxase | ||
Relaxase protein sequence
Download Length: 687 a.a. Molecular weight: 75762.89 Da Isoelectric Point: 4.4954
>WP_086353228.1 LPD7 domain-containing protein [Escherichia coli]
MNESAEPSPSPDAVKEEMTVMTEPDDKQSIGISPDTPREEAVNHVTEDIPLQPEPFLPESDGPEEYEDYS
AYQELMNNENPEHLQENSDMPSQPDTGHVQADESELQATTATIEPAVRDEPTPQQPVQSTSPSDNTSSFL
DKARGFFTRKKTDSQAHENSDPTPSAETTTTPPIPDSIVYAPERSDAPISLNMDEILKELDWEKRADRTV
LYKLDGKPAFIDRVNRLEMVNGASNDDRSVLAALAVATKFYGGVIELTGSDAFKQKAMQLIIEHNIDVRM
KLPDQRAELEKLRKEMAVSKDAVVTHQPTPELNRNIPEQPAVPDPVQEKEATQSPASPAAPEASTVSTAP
ASSPAEPGKTADAAPGEEPPGKLRPGESVTAVLHNFGRAEYAPGKGESFFVELKNRSGSKLYWGEQLESL
VKNHQKGDVVTLTLQNREQFILPGEQKARFRNKWSMESVTNGISVSHDNPDKGQRIQAIPVETFMKVAAQ
ISQGWPEEMKALRMPENVGSHLFIGEDRHPVSAPQNANQVTEITSAAPDKLTPVLGSVDKDTRELNLLLV
QSADEHLQGVVRLNGTLYPALATPSADNSQLVINALTDKGLRFAGYGEAVNHDADSTNRPAPELMQFHLK
TREEPLFAAVYTPEKQPDALYRNLGFEQSWQQWSNSQKPEDRQEKTLHQDLSHSPGR
MNESAEPSPSPDAVKEEMTVMTEPDDKQSIGISPDTPREEAVNHVTEDIPLQPEPFLPESDGPEEYEDYS
AYQELMNNENPEHLQENSDMPSQPDTGHVQADESELQATTATIEPAVRDEPTPQQPVQSTSPSDNTSSFL
DKARGFFTRKKTDSQAHENSDPTPSAETTTTPPIPDSIVYAPERSDAPISLNMDEILKELDWEKRADRTV
LYKLDGKPAFIDRVNRLEMVNGASNDDRSVLAALAVATKFYGGVIELTGSDAFKQKAMQLIIEHNIDVRM
KLPDQRAELEKLRKEMAVSKDAVVTHQPTPELNRNIPEQPAVPDPVQEKEATQSPASPAAPEASTVSTAP
ASSPAEPGKTADAAPGEEPPGKLRPGESVTAVLHNFGRAEYAPGKGESFFVELKNRSGSKLYWGEQLESL
VKNHQKGDVVTLTLQNREQFILPGEQKARFRNKWSMESVTNGISVSHDNPDKGQRIQAIPVETFMKVAAQ
ISQGWPEEMKALRMPENVGSHLFIGEDRHPVSAPQNANQVTEITSAAPDKLTPVLGSVDKDTRELNLLLV
QSADEHLQGVVRLNGTLYPALATPSADNSQLVINALTDKGLRFAGYGEAVNHDADSTNRPAPELMQFHLK
TREEPLFAAVYTPEKQPDALYRNLGFEQSWQQWSNSQKPEDRQEKTLHQDLSHSPGR
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 63963..84529
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| CA270_RS26225 (CA270_26200) | 61679..63970 | - | 2292 | WP_012783931 | F-type conjugative transfer protein TrbC | - |
| CA270_RS26230 (CA270_26205) | 63963..65033 | - | 1071 | WP_012783932 | IncI1-type conjugal transfer protein TrbB | trbB |
| CA270_RS26235 (CA270_26210) | 65052..66260 | - | 1209 | WP_001703845 | IncI1-type conjugal transfer protein TrbA | trbA |
| CA270_RS26240 (CA270_26215) | 66567..67346 | - | 780 | WP_275450201 | protein FinQ | - |
| CA270_RS26245 (CA270_26220) | 67973..68125 | + | 153 | WP_001387489 | Hok/Gef family protein | - |
| CA270_RS26250 (CA270_26225) | 68197..68448 | - | 252 | WP_001291964 | hypothetical protein | - |
| CA270_RS29520 | 68949..69044 | + | 96 | WP_000609148 | DinQ-like type I toxin DqlB | - |
| CA270_RS28820 | 69109..69285 | - | 177 | WP_001054900 | hypothetical protein | - |
| CA270_RS26260 (CA270_26235) | 69677..69886 | + | 210 | WP_000062603 | HEAT repeat domain-containing protein | - |
| CA270_RS26265 (CA270_26240) | 69958..70620 | - | 663 | WP_012783935 | plasmid IncI1-type surface exclusion protein ExcA | - |
| CA270_RS26270 (CA270_26245) | 70691..72858 | - | 2168 | Protein_85 | DotA/TraY family protein | - |
| CA270_RS26275 (CA270_26250) | 72955..73539 | - | 585 | WP_012783937 | conjugal transfer protein TraX | - |
| CA270_RS26280 (CA270_26255) | 73568..74770 | - | 1203 | WP_012783938 | IncI1-type conjugal transfer protein TraW | traW |
| CA270_RS26285 (CA270_26260) | 74737..75351 | - | 615 | WP_000337394 | IncI1-type conjugal transfer protein TraV | traV |
| CA270_RS26290 (CA270_26265) | 75351..78395 | - | 3045 | WP_012783939 | IncI1-type conjugal transfer protein TraU | traU |
| CA270_RS26295 (CA270_26270) | 78485..79285 | - | 801 | WP_001164788 | IncI1-type conjugal transfer protein TraT | traT |
| CA270_RS26300 (CA270_26275) | 79269..79457 | - | 189 | WP_001277255 | putative conjugal transfer protein TraS | - |
| CA270_RS26305 (CA270_26280) | 79521..79925 | - | 405 | WP_000086965 | IncI1-type conjugal transfer protein TraR | traR |
| CA270_RS26310 (CA270_26285) | 79976..80503 | - | 528 | WP_001055569 | conjugal transfer protein TraQ | traQ |
| CA270_RS26315 (CA270_26290) | 80503..81207 | - | 705 | WP_086353227 | IncI1-type conjugal transfer protein TraP | traP |
| CA270_RS26320 (CA270_26295) | 81207..82496 | - | 1290 | WP_011264042 | conjugal transfer protein TraO | traO |
| CA270_RS26325 (CA270_26300) | 82499..83482 | - | 984 | WP_001191880 | IncI1-type conjugal transfer protein TraN | traN |
| CA270_RS26330 (CA270_26305) | 83493..84185 | - | 693 | WP_000138552 | DotI/IcmL family type IV secretion protein | traM |
| CA270_RS26335 (CA270_26310) | 84182..84529 | - | 348 | WP_001055900 | conjugal transfer protein | traL |
Host bacterium
| ID | 1414 | GenBank | NZ_CP021208 |
| Plasmid name | p2474-3 | Incompatibility group | IncI1 |
| Plasmid size | 86725 bp | Coordinate of oriT [Strand] | 58494..58603 [-] |
| Host baterium | Escherichia coli strain strain Z247 |
Cargo genes
| Drug resistance gene | blaCTX-M-55 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrVA2 |