Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100955
Name   oriT_p1002-MCR1 in_silico
Organism   Escherichia coli strain Z1002
Sequence Completeness      core
NCBI accession of oriT (coordinates [strand])   NZ_CP021205 (28384..28436 [+], 53 nt)
oriT length   53 nt
IRs (inverted repeats)      5..21, 25..42  (CGATTGTAACATGACCG..CGGTCTTGTGTACAATCG)
Location of nic site      50..51
Conserved sequence flanking the
  nic site    		  TATCGTG|C
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 53 nt

>oriT_p1002-MCR1
CACACGATTGTAACATGACCGGAACGGTCTTGTGTACAATCGGTATCGTGCCT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   1025 GenBank   WP_034169409
Name   Putativerelaxaseofp1002-MCR1 insolico UniProt ID   A0A0R6LVD4
Length   1437 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 1437 a.a.        Molecular weight: 164649.28 Da        Isoelectric Point: 4.9461

>WP_034169409.1 MULTISPECIES: LPD7 domain-containing protein [Enterobacteriaceae]
MIVRYGGGNDGIVDYLINGRKAERQYTRDELDHRVVLDGDLQTTDKIIDSIENKSQERYLHITLSFHESH
VSNEVLKAVVDDYKKLLMNAYHPDEYSFYAEAHLPKIRHIQDNSTGELVERKPHIHIVIPKVNLITEKFL
NPVGDVTKGHTIEQLDAIQEFINNKYNLDSPKDYPRKDADYGKIISRVKGDLYKEHHSELKGELLSRIEN
EKIENYSVFKDIVAEYGELRIRNAGKTNEYLAVKLPGDKKYINLKSPLFRQNYIETRTLTLEKPTHKEIE
KRLNTWLNKTSQEIKHIFNQAEKTRELYKTLSPSQQIDFLQERIKEYDSREKLNERNSQQTSGRAGGYKS
CPKKFARIRQSEATVGLSRMPQRGMVYGINGFTRPDSVSVLSDISQRDLAEQLSQREHPGQDVRRDYDRQ
FTESGIKSLERSSFLCETMFQTLNEAAEKNEIATMAEIRRNIDPVRFLSSAAERFNIIPAQHKIRTAKDG
SPRFSVGNRNMNASDFLTKHINLAWKDAKSFLLEVYSQQLENTPYTRYPTYRRLTHHEARERLNSLNLSE
KTLRNTIRFERGKLYNDLREMRRELKLIPREQRDIAVGVIVYKKLTTLERLSELDTEGRHIIRQYHADWH
KDKDEMKALERLKSYLNFDEINAISADEPELSLQKAVDSQRRLEEAKKVNSKLKDLVMDKQDSRIVYRDQ
ESEKPVFTDKGNFVVAGKNPSKEEIGIMLEYSREKFGGVLKLTGSEDFKKMCAEVAAEQDMKIILRPEQY
QQMMLELKAELQGNKFEQVETQENSQESESRIEKGDALKEQATEQEQATEQAQATEQAQATEQAQATEQA
QATEQEQATEQEQVTEQEQATEQEQATEQAQATEQAQATEQAQVAAQATSSYDPGVITRANTLDSQMLSK
GTNGEFGYLKSLDSDENEIWEVLGHVPGDSDDIFDVASFDNENDAKEFCKIVNELGIDRTQALIQEQLTT
QHDQATAQVHNKQEIYCINFSRFHDLNEGIVFHSKDAAIQCYEESKSSAIEKYESNYLNGDGFDTVVLMS
KTVSTDELSSYPEGALVTFDRPFEIIANSYEEYRTPVYAVSFSKDEFSEDIKTFESLHDASEYKNKMLQE
HGLNQDDILITPVTREEIAFKGIKDAVNDANMAVMEQAGDSSRESPEEILASISANEHMISGLENFLVKD
RSQFSSCNGDIVVEAEITRGEGGLYHLAVAGKHGLERGDAVARVDVTEQQFAAITGKTPSEVLTGDQTSA
RVPVITGIHFSTRAIENVNKLEQQKDYVYFSTHEGLNAEIKDFSSLKDAIEWGRVECELHDLNKRDTVIY
RVESEHISQGIDAVMKNAERVERHEIEKAQGRDCTPEDGKILEAIDRFEDKFRGEGLKFEREKAESDLLN
HGFTREMAEDALGKQFVQAREEHLELQQQRDNSQDMH

  Protein domains


Predicted by InterproScan.

Large polyvalent protein-associated domain 7 (LPD7)

(692-774)


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 48487..62099

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CA268_RS28830 (CA268_28745) 44399..44851 + 453 WP_000101552 CaiF/GrlA family transcriptional regulator -
CA268_RS28835 (CA268_28750) 44844..45059 + 216 WP_001127357 DUF1187 family protein -
CA268_RS30740 45052..45228 + 177 WP_000753050 hypothetical protein -
CA268_RS28840 (CA268_28755) 45255..46208 + 954 WP_072089442 SPFH domain-containing protein -
CA268_RS28850 (CA268_28765) 46582..47025 + 444 WP_000964330 NfeD family protein -
CA268_RS30745 47029..47199 + 171 WP_000550720 hypothetical protein -
CA268_RS28855 (CA268_28770) 47210..47656 + 447 WP_001243165 hypothetical protein -
CA268_RS28860 (CA268_28775) 47689..47946 + 258 WP_001542015 hypothetical protein -
CA268_RS28865 (CA268_28780) 47927..48229 + 303 WP_001360345 hypothetical protein -
CA268_RS28870 (CA268_28785) 48226..48483 + 258 WP_000739144 hypothetical protein -
CA268_RS28875 (CA268_28790) 48487..49482 + 996 WP_001028543 type IV secretion system protein virB6
CA268_RS28880 (CA268_28795) 49488..50129 + 642 WP_001425343 type IV secretion system protein -
CA268_RS28885 (CA268_28800) 50131..50385 + 255 WP_001043555 EexN family lipoprotein -
CA268_RS28890 (CA268_28805) 50398..50685 + 288 WP_001032611 EexN family lipoprotein -
CA268_RS28895 (CA268_28810) 50758..51393 + 636 WP_015059536 hypothetical protein -
CA268_RS28900 (CA268_28815) 51493..51744 + 252 WP_000121741 hypothetical protein -
CA268_RS28905 (CA268_28820) 51734..52015 + 282 WP_000638823 type II toxin-antitoxin system RelE/ParE family toxin -
CA268_RS28910 (CA268_28825) 52083..52382 + 300 WP_000835764 TrbM/KikA/MpfK family conjugal transfer protein -
CA268_RS28915 (CA268_28830) 52385..53620 + 1236 WP_015059538 TcpQ domain-containing protein -
CA268_RS28920 (CA268_28835) 53626..54063 + 438 WP_000539665 type IV pilus biogenesis protein PilM -
CA268_RS28925 (CA268_28840) 54182..54580 + 399 WP_001153665 hypothetical protein -
CA268_RS28930 (CA268_28845) 54601..55185 + 585 WP_001177113 lytic transglycosylase domain-containing protein virB1
CA268_RS31340 55185..55475 + 291 WP_000865479 conjugal transfer protein -
CA268_RS28940 (CA268_28855) 55546..55866 + 321 WP_000362080 VirB3 family type IV secretion system protein virB3
CA268_RS28945 (CA268_28860) 55872..58229 + 2358 WP_063078682 VirB4 family type IV secretion system protein virb4
CA268_RS28955 (CA268_28870) 58395..59129 + 735 WP_000432282 type IV secretion system protein virB8
CA268_RS28960 (CA268_28875) 59195..59896 + 702 WP_000274524 TrbG/VirB9 family P-type conjugative transfer protein -
CA268_RS28965 (CA268_28880) 59886..61025 + 1140 WP_000790640 TrbI/VirB10 family protein virB10
CA268_RS28970 (CA268_28885) 61044..62099 + 1056 WP_001059977 P-type DNA transfer ATPase VirB11 virB11

Host bacterium

ID   1413 GenBank   NZ_CP021205
Plasmid name   p1002-MCR1 Incompatibility group   IncI2
Plasmid size   63392 bp Coordinate of oriT [Strand]   28384..28436 [+]
Host baterium   Escherichia coli strain Z1002

Cargo genes

Drug resistance gene   mcr-1.1
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -