Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 100954 |
| Name | oriT_p1002-1  |
| Organism | Escherichia coli strain Z1002 |
| Sequence Completeness | intact |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP021203 (137885..138434 [-], 550 nt) |
| oriT length | 550 nt |
| IRs (inverted repeats) | 243..255, 257..269 (TATTTAATAATTC..GAATTATTAAATA) 311..318, 321..328 (GCAAAAAC..GTTTTTGC) |
| Location of nic site | 337..338 |
| Conserved sequence flanking the nic site |
GTAGTGTGT|GG |
| Note | predicted by the oriTfinder |
oriT sequence
Download Length: 550 nt
>oriT_p1002-1
TTACTCTGGCCATAAGATAAAACCTTTCATTATTAAGCAATGAACTTTTCACTATAAATATGCATATAGTGTTTACAAGTAAGAAAGACACTCCTAGCAGCGCCTCTAGGATCATCCTATAAAAAAATGCGATCCGGCGCTAGGGGCGTCCCTAATATATATCAATGTTTTTCATGAAAATTGTCAGTACTGATCCTAATAAGAGTCGCTATAGGGTCGTAACAGGATCGCCAACGACTCTCTATTTAATAATTCAGAATTATTAAATATAAATAGCGTTTGTTAATTACATGATTTAAAACGTAAATCAGCAAAAACTTGTTTTTGCGTAGTGTGTGGTGCTTTTGGTGGTGAGAACCACCAACCTGTTGAGCCTTTTTGTGGAGTGGGTTAAATTATTTACGGATAAAGTCACCAGAGGTGGAAAAATGAAAAAATGGATGTTAGCAATCTGCCTGATGTTTATAAATGGGATCTGCGAAGCCGCCGATTGCTTTGATCTTGCAGGTCGGGATTACAAAATAGACCCGGATTTACTGAGAGCGATATC
TTACTCTGGCCATAAGATAAAACCTTTCATTATTAAGCAATGAACTTTTCACTATAAATATGCATATAGTGTTTACAAGTAAGAAAGACACTCCTAGCAGCGCCTCTAGGATCATCCTATAAAAAAATGCGATCCGGCGCTAGGGGCGTCCCTAATATATATCAATGTTTTTCATGAAAATTGTCAGTACTGATCCTAATAAGAGTCGCTATAGGGTCGTAACAGGATCGCCAACGACTCTCTATTTAATAATTCAGAATTATTAAATATAAATAGCGTTTGTTAATTACATGATTTAAAACGTAAATCAGCAAAAACTTGTTTTTGCGTAGTGTGTGGTGCTTTTGGTGGTGAGAACCACCAACCTGTTGAGCCTTTTTGTGGAGTGGGTTAAATTATTTACGGATAAAGTCACCAGAGGTGGAAAAATGAAAAAATGGATGTTAGCAATCTGCCTGATGTTTATAAATGGGATCTGCGAAGCCGCCGATTGCTTTGATCTTGCAGGTCGGGATTACAAAATAGACCCGGATTTACTGAGAGCGATATC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 1024 | GenBank | WP_000130000 |
| Name | Relaxase_p1002-1 |
UniProt ID | Q0ZKT4 |
| Length | 101 a.a. | PDB ID | |
| Note | putative relaxase | ||
Relaxase protein sequence
Download Length: 101 a.a. Molecular weight: 11477.11 Da Isoelectric Point: 7.5204
>WP_000130000.1 MULTISPECIES: PadR family transcriptional regulator [Pseudomonadota]
MTDKDLYGGLIRLHILHHAAEEPVFGLGIIEELRRHGYEMSAGTVYPMLHGLEKKGYLTSRHERTGRRER
RVYDITEQGRTALADAKTKVKELFGELVEGG
MTDKDLYGGLIRLHILHHAAEEPVFGLGIIEELRRHGYEMSAGTVYPMLHGLEKKGYLTSRHERTGRRER
RVYDITEQGRTALADAKTKVKELFGELVEGG
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | Q0ZKT4 |
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 137497..165549
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| CA268_RS27545 (CA268_27475) | 133469..133903 | + | 435 | WP_000845953 | conjugation system SOS inhibitor PsiB | - |
| CA268_RS27550 (CA268_27480) | 133900..134662 | + | 763 | Protein_150 | plasmid SOS inhibition protein A | - |
| CA268_RS30705 (CA268_27485) | 134640..134819 | - | 180 | WP_001309233 | hypothetical protein | - |
| CA268_RS31260 | 134841..134990 | + | 150 | Protein_152 | plasmid maintenance protein Mok | - |
| CA268_RS27560 (CA268_27490) | 134932..135057 | + | 126 | WP_001372321 | type I toxin-antitoxin system Hok family toxin | - |
| CA268_RS30810 | 135376..135672 | - | 297 | Protein_154 | hypothetical protein | - |
| CA268_RS27580 (CA268_27510) | 135972..136268 | + | 297 | WP_001272251 | hypothetical protein | - |
| CA268_RS27585 (CA268_27515) | 136379..137200 | + | 822 | WP_001234445 | DUF932 domain-containing protein | - |
| CA268_RS27590 (CA268_27520) | 137497..138099 | - | 603 | WP_000243713 | transglycosylase SLT domain-containing protein | virB1 |
| CA268_RS27595 (CA268_27525) | 138422..138805 | + | 384 | WP_001354030 | conjugal transfer relaxosome DNA-binding protein TraM | - |
| CA268_RS27600 (CA268_27530) | 138999..139670 | + | 672 | WP_000283561 | conjugal transfer transcriptional regulator TraJ | - |
| CA268_RS27605 (CA268_27535) | 139807..140034 | + | 228 | WP_000089263 | conjugal transfer relaxosome protein TraY | - |
| CA268_RS27610 (CA268_27540) | 140067..140425 | + | 359 | Protein_161 | type IV conjugative transfer system pilin TraA | - |
| CA268_RS27615 (CA268_27545) | 140440..140751 | + | 312 | WP_000012113 | type IV conjugative transfer system protein TraL | traL |
| CA268_RS27620 (CA268_27550) | 140773..141339 | + | 567 | WP_000399780 | type IV conjugative transfer system protein TraE | traE |
| CA268_RS27625 (CA268_27555) | 141326..142054 | + | 729 | WP_001230772 | type-F conjugative transfer system secretin TraK | traK |
| CA268_RS27630 (CA268_27560) | 142054..143505 | + | 1452 | WP_000146675 | F-type conjugal transfer pilus assembly protein TraB | traB |
| CA268_RS27635 (CA268_27565) | 143495..144061 | + | 567 | WP_000896599 | conjugal transfer pilus-stabilizing protein TraP | - |
| CA268_RS27640 (CA268_27570) | 144048..144368 | + | 321 | WP_001057307 | conjugal transfer protein TrbD | - |
| CA268_RS27645 (CA268_27575) | 144365..144880 | + | 516 | WP_000809881 | type IV conjugative transfer system lipoprotein TraV | traV |
| CA268_RS27650 (CA268_27580) | 145015..145236 | + | 222 | WP_001278683 | conjugal transfer protein TraR | - |
| CA268_RS27655 (CA268_27585) | 145229..145705 | + | 477 | WP_000549587 | hypothetical protein | - |
| CA268_RS27660 (CA268_27590) | 145785..146003 | + | 219 | WP_000556745 | hypothetical protein | - |
| CA268_RS30370 | 146031..146378 | + | 348 | WP_000836682 | hypothetical protein | - |
| CA268_RS27670 (CA268_27600) | 146504..149134 | + | 2631 | WP_000069777 | type IV secretion system protein TraC | virb4 |
| CA268_RS27675 (CA268_27605) | 149131..149517 | + | 387 | WP_000214084 | type-F conjugative transfer system protein TrbI | - |
| CA268_RS27680 (CA268_27610) | 149514..150146 | + | 633 | WP_001203728 | type-F conjugative transfer system protein TraW | traW |
| CA268_RS27685 (CA268_27615) | 150143..151135 | + | 993 | WP_000830838 | conjugal transfer pilus assembly protein TraU | traU |
| CA268_RS27690 (CA268_27620) | 151165..151470 | + | 306 | WP_000224416 | hypothetical protein | - |
| CA268_RS27695 (CA268_27625) | 151479..152117 | + | 639 | WP_001080256 | type-F conjugative transfer system pilin assembly protein TrbC | trbC |
| CA268_RS27700 (CA268_27630) | 152114..153964 | + | 1851 | WP_000821856 | type-F conjugative transfer system mating-pair stabilization protein TraN | traN |
| CA268_RS27705 (CA268_27635) | 153991..154248 | + | 258 | WP_000864353 | conjugal transfer protein TrbE | - |
| CA268_RS27710 (CA268_27640) | 154241..154984 | + | 744 | WP_001030371 | type-F conjugative transfer system pilin assembly protein TraF | traF |
| CA268_RS27715 (CA268_27645) | 154998..155342 | + | 345 | WP_000556796 | conjugal transfer protein TrbA | - |
| CA268_RS27720 (CA268_27650) | 155461..155745 | + | 285 | WP_000624194 | type-F conjugative transfer system pilin chaperone TraQ | - |
| CA268_RS27725 (CA268_27655) | 155732..156277 | + | 546 | WP_000059831 | type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB | traF |
| CA268_RS27730 (CA268_27660) | 156207..156554 | + | 348 | WP_001309242 | P-type conjugative transfer protein TrbJ | - |
| CA268_RS27735 (CA268_27665) | 156535..156927 | + | 393 | WP_000660699 | F-type conjugal transfer protein TrbF | - |
| CA268_RS27740 (CA268_27670) | 156914..158287 | + | 1374 | WP_000944331 | conjugal transfer pilus assembly protein TraH | traH |
| CA268_RS27745 (CA268_27675) | 158284..161106 | + | 2823 | WP_086352862 | conjugal transfer mating-pair stabilization protein TraG | traG |
| CA268_RS27750 (CA268_27680) | 161122..161607 | + | 486 | WP_000605870 | hypothetical protein | - |
| CA268_RS27755 (CA268_27685) | 161656..162387 | + | 732 | WP_000782451 | conjugal transfer complement resistance protein TraT | - |
| CA268_RS27760 (CA268_27690) | 162590..163327 | + | 738 | WP_000199905 | hypothetical protein | - |
| CA268_RS27765 (CA268_27695) | 163378..165549 | + | 2172 | WP_023910159 | type IV conjugative transfer system coupling protein TraD | virb4 |
Host bacterium
| ID | 1412 | GenBank | NZ_CP021203 |
| Plasmid name | p1002-1 | Incompatibility group | p0111 |
| Plasmid size | 183508 bp | Coordinate of oriT [Strand] | 137885..138434 [-] |
| Host baterium | Escherichia coli strain Z1002 |
Cargo genes
| Drug resistance gene | tellurite/colicin resistance; tunicamycin resistance |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |