Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100953
Name   oriT_pSCC4 in_silico
Organism   Citrobacter braakii strain SCC4
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NZ_CP021078 (3036..3138 [-], 103 nt)
oriT length   103 nt
IRs (inverted repeats)      53..68, 75..90  (GTAAAGTTGGACCACC..GGTGGGCCTACTT)
Location of nic site      98..99
Conserved sequence flanking the
  nic site    		  TATCCTG|C
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 103 nt

>oriT_pSCC4
AGCCTCGCAGAGCAGGATTCCCGTTGAGCAGCCCAGCTGCGAATAAGGGAAGGTAAAGTTGGACCACCGTTTACGGTGGGCCTACTTTACATATCCTGCCCGG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   1023 GenBank   WP_050580356
Name   TraI_pSCC4 insolico UniProt ID   _
Length   869 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 869 a.a.        Molecular weight: 96507.42 Da        Isoelectric Point: 10.4435

>WP_050580356.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacteriaceae]
MKNIKKSSFSGLADYITNPQSKQERVGVIKATNCQSQDVQDAVTEVNITQRLNTRSESDKTYHLVISFRP
GEKPSEDVLNAIEESICQSLGFGDHQRLSAVHHDTDNLHIHVAINKINPDTLTIHNPYNDYRVLAESCAY
LEKKYGLEVDNHMPTRSASENRAKDMERHSGIQSLLSWVKAECAEQLLNANSWQELHQAMRQNGLELRQK
GNGFVIAGPDGLAVKASSVDRSLSRAALEKKLGSFESSIPDTKPSRPSPEAIKQRFGSAANVKRPGDRPP
PMRENRQVHLGDVPVMQINSGKRYESKPLGSQGANTTELYGRYQSEQQRLTAARNEALRLAKVKRDRLIE
NAKRSGRLKRATIKLLKGPGVNKRYLYGLASKALGEELERANAIYKKDRQAAYDIHKRRTWADWLQFKAA
GGDDEALKTLRQRNAQQARKEANILHGDKVKKDGRIPGLKPDSVTKEGTIIYRVGESAIRDGGKRLDVSR
DAGDDGLHAALLMAKHRYGDKLTVNGSFEFKKRIVAVAAARELDVSFADPELEKLRQILTKKAAREREYI
HIPFEKRGKARQAGAMWDTEKKSWFVGPYATRDRIENFIRMNEEKDNEQRRQRYAGTERTDAGRERSGNG
NATGANEPGVTGRSTSADAGNGYGTSSSRRRTGTGDAVTTAGSQPGRSDDTRTGRSRAAEPNGERGHSKP
NVARPGRQPPPTAKNRLRAMSELGMVRIAGGSEVLLQGNVSDQLEHKTAERADQLRRDMAGAGGITTPAE
VKKPLSSAEKYINEREQKRLKGFDIPKHRGYNSTDKGLFEYAGQRKSGLETLALLKRDDEIVVMPVSDYT
ARRLSNLKVGESVTLTPSGSVRTTKGRSR

  Protein domains


Predicted by InterproScan.

Domain of unknown function (DUF5710) (DUF5710)

(557-598)

Large polyvalent protein-associated domain 7 (LPD7)

(463-547)

Relaxase/Mobilisation nuclease domain (Relaxase)

(5-243)


  Protein structure



No available structure.




Host bacterium

ID   1411 GenBank   NZ_CP021078
Plasmid name   pSCC4 Incompatibility group   IncP1
Plasmid size   44155 bp Coordinate of oriT [Strand]   3036..3138 [-]
Host baterium   Citrobacter braakii strain SCC4

Cargo genes

Drug resistance gene   mcr-1.1
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -