Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100946
Name   oriT_pLd2 in_silico
Organism   Lactococcus lactis subsp. lactis bv. diacetylactis strain FM03
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NZ_CP020606 (9180..9596 [+], 417 nt)
oriT length   417 nt
IRs (inverted repeats)      IR1: 161..173, 177..189  (AAAAGGGGAAAGT..ACTTCCCCCTTTT)
  IR2: 195..209, 222..236  (CACATTGTAATACAA..TTGTATTACAATGTG)
Location of nic site      244..245
Conserved sequence flanking the
  nic site    		  CTTG|CA
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 417 nt

>oriT_pLd2
CCATGGTTTTAGAAAAAAGAGGGACGATTTCGGAAGACGAAAATCGTCTCTTTTTTTCTTCTTTTTGTATGACAAAAAGAAAGATCTTTTGCCCATTTTATTTTTATCAAATGGGCATTTGGCGTTTGCGTAAAGCAAATCGACACAATCCAAAGGGGATAAAAGGGGAAAGTGAAACTTCCCCCTTTTCAAGCCACATTGTAATACAAGAACGAAGTGCTTTGTATTACAATGTGATAGCTTGCAGTATTTCTGGTTTTATATGGTCTATTTTGTTATAATGATTGTAACCGAATAGGGCGCAATGCTTATTACAAAATCAATGACAAAGGGCGATTGAGGAATGAGCGCTGGGGCATTTTATCTTTGAGGAGGCTATTTATGGATCAAAAAGAAGTATCACAAAATCAAACAAAG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   1016 GenBank   WP_086383686
Name   Relaxase_pLd2 insolico UniProt ID   _
Length   410 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 410 a.a.        Molecular weight: 47785.43 Da        Isoelectric Point: 8.6734

>WP_086383686.1 relaxase/mobilization nuclease domain-containing protein [Lactococcus lactis]
MATIAKISNGASAASALNYALGQDRPMHEKTEQWLQDHQLERPVELTNCRAVAVGGTNGIDPFIAKEQFD
VIRQLHNQTKESNQVLRITQSFALDELNPKVQKDWQKANDLGVELAENLYPNHQSAVYTHLDGKNHVLHN
HIIVNKVNLETGKKLREQKGESVQRAREMNDRLASRENWHILEPPKERQTETEKELIAKNEYSYMDDLRE
RINKSLQDVSVSSYETFKERLSDNGVILSERGQTFSYAFLDANNKQRRARETRLGSDFGRETILHELENR
ARQNEFRAVEQREPAITPLERDTQQRESEIVSLEQAIEPRKSEALKRESKINRFIDTIKQFAGRVPELTQ
RVTRKLKQTKEKILDDFERRFSKDMKNYEQEQQKSLEKQANRDVQSEKKPTKDHDRGMSR

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(9-280)


  Protein structure



No available structure.




Host bacterium

ID   1404 GenBank   NZ_CP020606
Plasmid name   pLd2 Incompatibility group   _
Plasmid size   15218 bp Coordinate of oriT [Strand]   9180..9596 [+]
Host baterium   Lactococcus lactis subsp. lactis bv. diacetylactis strain FM03

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -