Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100943
Name   oriT_unitig_5-pAR_0117 in_silico
Organism   Klebsiella pneumoniae strain AR_0117
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NZ_CP020066 (11416..12042 [+], 627 nt)
oriT length   627 nt
IRs (inverted repeats)      IR1: 198..207, 211..220  (ATTGGGTGTT..AATACCTAAT)
 IR2:319..324, 330..335  (AAAAAA..TTTTTT)
  IR3: 393..402, 405..414  (GTATTCATGC..GCATGAATAC)
Location of nic site      301..302
Conserved sequence flanking the
  nic site    		  GGTGT|ATAGC
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 627 nt

>oriT_unitig_5-pAR_0117
AGCGCCGCAGATAATCTGACCGATTACCTCCTGAAACCAGGTCTATATAGGCCAAAAGTTCATCTGATACTTTTGCGGTTATTATTGGCATTCAGTCCTCACATTGTGCATTTTTTAAACAAAAAATTGGGATCTAACAAGCTGAAATCTTAGTATTACCAAAGTAATAAAGCAAACTCATTATAAAACAATGAGTTATTGGGTGTTTTTAATACCTAATTATTACCGAATATTGTTGCTATTTATTTTTTTATCTTTTAAATCAGTATGATAGCGTGATTTATCGCGCTGCGTTAGGTGTATAGCAGGTTAAGGGATAAAAAATCATCTTTTTTGGTAGGGGCGATCTACGTAGGTTAAGGACTAACTGGCTAAAAAGCGTTCAATATTCCGTATTCATGCTTGCATGAATACCAGTACAACAAAAGTACATCAAAATTACATCAAAATTACATCACTTGAAGGTTGACAGTACAACAAAATTACATCATTCTTTGGTCATGAGGTAGCCAGTACAACAAAAGTACATCAAAAGTACATCAAAATTACATCAAAATTACATCATTCTAAATGAGGGTACTATGAAGCCCAAAAGTATCAGGGCGGCACTTCAGTTGATGTTGCCGG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   1013 GenBank   WP_012561166
Name   Relaxaseofunitig_5-pAR_0117 insolico UniProt ID   _
Length   1078 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 1078 a.a.        Molecular weight: 120141.30 Da        Isoelectric Point: 6.5229

>WP_012561166.1 MULTISPECIES: MobF family relaxase [Gammaproteobacteria]
MLDITTITRQNVTSVVGYYSDAKDDYYSKDSSFTSWQGTGAEALGLSGDVESARFKELLVGEIDTFTHMQ
RHVGDAKKERLGYDLTFSAPKGVSMQALIHGDKTIIEAHEKAVAAAVREAEKLAQARTTRQGKSVTQNTN
NLVVATFRHETSRALDPDLHTHAFVMNMTQREDGQWRALKNDELMRNKMHLGDVYKQELALELTKAGYEL
RYNSKNNTFDMAHFSDEQIRAFSRRSEQIEKGLAAMGLTRETADAQTKSRVSMATREKKTEHSREEIHQE
WASRAKTLGIDFDNREWQGHGKPLEADIARNMAPDFTSPEVKADRAIQFAVKSLSERDASFERQKLIQIA
NKQVLGHATMADVEKAYLKAVQKGAIIEGEARYQSTLKVGASVMAETLTRKEWIDSLTNSGMRADKARFA
VDDGIKNGRLKKTSHRVTTVEGIRLERSILTIESRGRGQMPRQLTAEIAGQLLAGKTLKNEQMRAVTEIV
TSKDRFVAAHGYAGTGKSYMTMAAKELLESQGLKVTALAPYGTQKKALEDDGLPARTVAAFLKAKDKKLD
EKSVVFIDEAGVIPARQMKQLMEVIEKHNARAVFLGDTSQTKAVEAGKPFEQLIKAGMQTSYMKDIQRQK
NEVLLEAVKYAAEGNAARALKNITGVNELKEEAPRLAQLADRYLSLSSEQQDATLIISGTNASRKTLNDY
IRGNLGLAGTGETFTLLDRVDSTQAERRDSRYFSKGQIIIPEQDYKNGMKRGESYQVLDTGPGNKLTVES
SSGEQIAFSPRTHTKLSVYQAVSAELAPGDKVMVTRNDKTLDVANGDRFTVKTVEGEKLTLEDKKGRTVE
LDKKQASYLSYAYATTVHKSQGLTCDRVLFNIDTKSLTTSKDVFYVGISRARHEVEIFTDDKKSLASSVS
RDSPKTTAAEIDRFFGLEARFKDIGRDTSLETRSAEKGLPEATGESMAFNQKPDEHNMTTGTDYQPVSNA
EDAFHLKQNPMDDSVGLRRHEAQQNDAELAHDYAAADDQQWSAQEYADYEHYAEASDYDFDSSIYDDYAM
PQTSQAEQSHTGKEHTHEHEHEEGGHEI

  Protein domains


Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C_2)

(851-895)

TrwC relaxase (TrwC)

(16-287)

AAA domain (AAA_30)

(477-653)


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 37163..47488

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM364_RS28960 (AM364_30110) 33008..34441 - 1434 WP_001288432 DNA cytosine methyltransferase -
AM364_RS28965 (AM364_30115) 34540..34812 + 273 Protein_41 IS1 family transposase -
AM364_RS28970 (AM364_30120) 34823..35029 - 207 WP_001749967 hypothetical protein -
AM364_RS28975 (AM364_30125) 35034..35681 - 648 WP_012561184 restriction endonuclease -
AM364_RS28980 (AM364_30130) 35731..35964 - 234 WP_001191790 hypothetical protein -
AM364_RS28985 (AM364_30135) 36078..36392 - 315 WP_001749965 TrbM/KikA/MpfK family conjugal transfer protein -
AM364_RS28990 (AM364_30140) 36389..36733 - 345 WP_001749964 hypothetical protein -
AM364_RS28995 (AM364_30145) 36749..37054 - 306 WP_000960954 H-NS family nucleoid-associated regulatory protein -
AM364_RS29000 (AM364_30150) 37163..37897 + 735 WP_001749963 lytic transglycosylase domain-containing protein virB1
AM364_RS29005 (AM364_30155) 37906..38187 + 282 WP_000440698 transcriptional repressor KorA -
AM364_RS29010 (AM364_30160) 38197..38490 + 294 WP_001749962 hypothetical protein virB2
AM364_RS29015 (AM364_30165) 38540..38857 + 318 WP_000496058 VirB3 family type IV secretion system protein virB3
AM364_RS29020 (AM364_30170) 38857..41457 + 2601 WP_001749961 VirB4 family type IV secretion/conjugal transfer ATPase virb4
AM364_RS29025 (AM364_30175) 41475..42188 + 714 WP_001749960 type IV secretion system protein virB5
AM364_RS29030 (AM364_30180) 42196..42423 + 228 WP_001749959 IncN-type entry exclusion lipoprotein EexN -
AM364_RS29035 (AM364_30185) 42439..43479 + 1041 WP_001749958 type IV secretion system protein virB6
AM364_RS29045 (AM364_30195) 43698..44396 + 699 WP_000646594 type IV secretion system protein virB8
AM364_RS29050 (AM364_30200) 44407..45291 + 885 WP_000735066 TrbG/VirB9 family P-type conjugative transfer protein virB9
AM364_RS29055 (AM364_30205) 45291..46451 + 1161 WP_000101710 type IV secretion system protein VirB10 virB10
AM364_RS29060 (AM364_30210) 46493..47488 + 996 WP_000128596 ATPase, T2SS/T4P/T4SS family virB11
AM364_RS29065 (AM364_30215) 47488..48021 + 534 WP_000792636 phospholipase D family protein -
AM364_RS30640 48195..48482 - 288 Protein_61 DUF6710 family protein -
AM364_RS29075 (AM364_30225) 48749..48928 + 180 Protein_62 helix-turn-helix domain-containing protein -
AM364_RS29080 (AM364_30230) 48993..49553 + 561 Protein_63 IS6-like element IS26 family transposase -
AM364_RS29085 (AM364_30235) 49573..51684 - 2112 Protein_64 Tn3-like element Tn3 family transposase -

Host bacterium

ID   1401 GenBank   NZ_CP020066
Plasmid name   unitig_5-pAR_0117 Incompatibility group   IncN
Plasmid size   72663 bp Coordinate of oriT [Strand]   11416..12042 [+]
Host baterium   Klebsiella pneumoniae strain AR_0117

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -