Detailed information of oriT
oriT
The information of the oriT region
oriTDB ID | 100943 |
Name | oriT_unitig_5-pAR_0117 |
Organism | Klebsiella pneumoniae strain AR_0117 |
Sequence Completeness | intact |
NCBI accession of oriT (coordinates [strand]) | NZ_CP020066 (11416..12042 [+], 627 nt) |
oriT length | 627 nt |
IRs (inverted repeats) | IR1: 198..207, 211..220 (ATTGGGTGTT..AATACCTAAT) IR2:319..324, 330..335 (AAAAAA..TTTTTT) IR3: 393..402, 405..414 (GTATTCATGC..GCATGAATAC) |
Location of nic site | 301..302 |
Conserved sequence flanking the nic site |
GGTGT|ATAGC |
Note | predicted by the oriTfinder |
oriT sequence
Download Length: 627 nt
>oriT_unitig_5-pAR_0117
AGCGCCGCAGATAATCTGACCGATTACCTCCTGAAACCAGGTCTATATAGGCCAAAAGTTCATCTGATACTTTTGCGGTTATTATTGGCATTCAGTCCTCACATTGTGCATTTTTTAAACAAAAAATTGGGATCTAACAAGCTGAAATCTTAGTATTACCAAAGTAATAAAGCAAACTCATTATAAAACAATGAGTTATTGGGTGTTTTTAATACCTAATTATTACCGAATATTGTTGCTATTTATTTTTTTATCTTTTAAATCAGTATGATAGCGTGATTTATCGCGCTGCGTTAGGTGTATAGCAGGTTAAGGGATAAAAAATCATCTTTTTTGGTAGGGGCGATCTACGTAGGTTAAGGACTAACTGGCTAAAAAGCGTTCAATATTCCGTATTCATGCTTGCATGAATACCAGTACAACAAAAGTACATCAAAATTACATCAAAATTACATCACTTGAAGGTTGACAGTACAACAAAATTACATCATTCTTTGGTCATGAGGTAGCCAGTACAACAAAAGTACATCAAAAGTACATCAAAATTACATCAAAATTACATCATTCTAAATGAGGGTACTATGAAGCCCAAAAGTATCAGGGCGGCACTTCAGTTGATGTTGCCGG
AGCGCCGCAGATAATCTGACCGATTACCTCCTGAAACCAGGTCTATATAGGCCAAAAGTTCATCTGATACTTTTGCGGTTATTATTGGCATTCAGTCCTCACATTGTGCATTTTTTAAACAAAAAATTGGGATCTAACAAGCTGAAATCTTAGTATTACCAAAGTAATAAAGCAAACTCATTATAAAACAATGAGTTATTGGGTGTTTTTAATACCTAATTATTACCGAATATTGTTGCTATTTATTTTTTTATCTTTTAAATCAGTATGATAGCGTGATTTATCGCGCTGCGTTAGGTGTATAGCAGGTTAAGGGATAAAAAATCATCTTTTTTGGTAGGGGCGATCTACGTAGGTTAAGGACTAACTGGCTAAAAAGCGTTCAATATTCCGTATTCATGCTTGCATGAATACCAGTACAACAAAAGTACATCAAAATTACATCAAAATTACATCACTTGAAGGTTGACAGTACAACAAAATTACATCATTCTTTGGTCATGAGGTAGCCAGTACAACAAAAGTACATCAAAAGTACATCAAAATTACATCAAAATTACATCATTCTAAATGAGGGTACTATGAAGCCCAAAAGTATCAGGGCGGCACTTCAGTTGATGTTGCCGG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure fileRelaxase
ID | 1013 | GenBank | WP_012561166 |
Name | Relaxaseofunitig_5-pAR_0117 | UniProt ID | _ |
Length | 1078 a.a. | PDB ID | |
Note | putative relaxase |
Relaxase protein sequence
Download Length: 1078 a.a. Molecular weight: 120141.30 Da Isoelectric Point: 6.5229
>WP_012561166.1 MULTISPECIES: MobF family relaxase [Gammaproteobacteria]
MLDITTITRQNVTSVVGYYSDAKDDYYSKDSSFTSWQGTGAEALGLSGDVESARFKELLVGEIDTFTHMQ
RHVGDAKKERLGYDLTFSAPKGVSMQALIHGDKTIIEAHEKAVAAAVREAEKLAQARTTRQGKSVTQNTN
NLVVATFRHETSRALDPDLHTHAFVMNMTQREDGQWRALKNDELMRNKMHLGDVYKQELALELTKAGYEL
RYNSKNNTFDMAHFSDEQIRAFSRRSEQIEKGLAAMGLTRETADAQTKSRVSMATREKKTEHSREEIHQE
WASRAKTLGIDFDNREWQGHGKPLEADIARNMAPDFTSPEVKADRAIQFAVKSLSERDASFERQKLIQIA
NKQVLGHATMADVEKAYLKAVQKGAIIEGEARYQSTLKVGASVMAETLTRKEWIDSLTNSGMRADKARFA
VDDGIKNGRLKKTSHRVTTVEGIRLERSILTIESRGRGQMPRQLTAEIAGQLLAGKTLKNEQMRAVTEIV
TSKDRFVAAHGYAGTGKSYMTMAAKELLESQGLKVTALAPYGTQKKALEDDGLPARTVAAFLKAKDKKLD
EKSVVFIDEAGVIPARQMKQLMEVIEKHNARAVFLGDTSQTKAVEAGKPFEQLIKAGMQTSYMKDIQRQK
NEVLLEAVKYAAEGNAARALKNITGVNELKEEAPRLAQLADRYLSLSSEQQDATLIISGTNASRKTLNDY
IRGNLGLAGTGETFTLLDRVDSTQAERRDSRYFSKGQIIIPEQDYKNGMKRGESYQVLDTGPGNKLTVES
SSGEQIAFSPRTHTKLSVYQAVSAELAPGDKVMVTRNDKTLDVANGDRFTVKTVEGEKLTLEDKKGRTVE
LDKKQASYLSYAYATTVHKSQGLTCDRVLFNIDTKSLTTSKDVFYVGISRARHEVEIFTDDKKSLASSVS
RDSPKTTAAEIDRFFGLEARFKDIGRDTSLETRSAEKGLPEATGESMAFNQKPDEHNMTTGTDYQPVSNA
EDAFHLKQNPMDDSVGLRRHEAQQNDAELAHDYAAADDQQWSAQEYADYEHYAEASDYDFDSSIYDDYAM
PQTSQAEQSHTGKEHTHEHEHEEGGHEI
MLDITTITRQNVTSVVGYYSDAKDDYYSKDSSFTSWQGTGAEALGLSGDVESARFKELLVGEIDTFTHMQ
RHVGDAKKERLGYDLTFSAPKGVSMQALIHGDKTIIEAHEKAVAAAVREAEKLAQARTTRQGKSVTQNTN
NLVVATFRHETSRALDPDLHTHAFVMNMTQREDGQWRALKNDELMRNKMHLGDVYKQELALELTKAGYEL
RYNSKNNTFDMAHFSDEQIRAFSRRSEQIEKGLAAMGLTRETADAQTKSRVSMATREKKTEHSREEIHQE
WASRAKTLGIDFDNREWQGHGKPLEADIARNMAPDFTSPEVKADRAIQFAVKSLSERDASFERQKLIQIA
NKQVLGHATMADVEKAYLKAVQKGAIIEGEARYQSTLKVGASVMAETLTRKEWIDSLTNSGMRADKARFA
VDDGIKNGRLKKTSHRVTTVEGIRLERSILTIESRGRGQMPRQLTAEIAGQLLAGKTLKNEQMRAVTEIV
TSKDRFVAAHGYAGTGKSYMTMAAKELLESQGLKVTALAPYGTQKKALEDDGLPARTVAAFLKAKDKKLD
EKSVVFIDEAGVIPARQMKQLMEVIEKHNARAVFLGDTSQTKAVEAGKPFEQLIKAGMQTSYMKDIQRQK
NEVLLEAVKYAAEGNAARALKNITGVNELKEEAPRLAQLADRYLSLSSEQQDATLIISGTNASRKTLNDY
IRGNLGLAGTGETFTLLDRVDSTQAERRDSRYFSKGQIIIPEQDYKNGMKRGESYQVLDTGPGNKLTVES
SSGEQIAFSPRTHTKLSVYQAVSAELAPGDKVMVTRNDKTLDVANGDRFTVKTVEGEKLTLEDKKGRTVE
LDKKQASYLSYAYATTVHKSQGLTCDRVLFNIDTKSLTTSKDVFYVGISRARHEVEIFTDDKKSLASSVS
RDSPKTTAAEIDRFFGLEARFKDIGRDTSLETRSAEKGLPEATGESMAFNQKPDEHNMTTGTDYQPVSNA
EDAFHLKQNPMDDSVGLRRHEAQQNDAELAHDYAAADDQQWSAQEYADYEHYAEASDYDFDSSIYDDYAM
PQTSQAEQSHTGKEHTHEHEHEEGGHEI
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 37163..47488
Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
---|---|---|---|---|---|---|
AM364_RS28960 (AM364_30110) | 33008..34441 | - | 1434 | WP_001288432 | DNA cytosine methyltransferase | - |
AM364_RS28965 (AM364_30115) | 34540..34812 | + | 273 | Protein_41 | IS1 family transposase | - |
AM364_RS28970 (AM364_30120) | 34823..35029 | - | 207 | WP_001749967 | hypothetical protein | - |
AM364_RS28975 (AM364_30125) | 35034..35681 | - | 648 | WP_012561184 | restriction endonuclease | - |
AM364_RS28980 (AM364_30130) | 35731..35964 | - | 234 | WP_001191790 | hypothetical protein | - |
AM364_RS28985 (AM364_30135) | 36078..36392 | - | 315 | WP_001749965 | TrbM/KikA/MpfK family conjugal transfer protein | - |
AM364_RS28990 (AM364_30140) | 36389..36733 | - | 345 | WP_001749964 | hypothetical protein | - |
AM364_RS28995 (AM364_30145) | 36749..37054 | - | 306 | WP_000960954 | H-NS family nucleoid-associated regulatory protein | - |
AM364_RS29000 (AM364_30150) | 37163..37897 | + | 735 | WP_001749963 | lytic transglycosylase domain-containing protein | virB1 |
AM364_RS29005 (AM364_30155) | 37906..38187 | + | 282 | WP_000440698 | transcriptional repressor KorA | - |
AM364_RS29010 (AM364_30160) | 38197..38490 | + | 294 | WP_001749962 | hypothetical protein | virB2 |
AM364_RS29015 (AM364_30165) | 38540..38857 | + | 318 | WP_000496058 | VirB3 family type IV secretion system protein | virB3 |
AM364_RS29020 (AM364_30170) | 38857..41457 | + | 2601 | WP_001749961 | VirB4 family type IV secretion/conjugal transfer ATPase | virb4 |
AM364_RS29025 (AM364_30175) | 41475..42188 | + | 714 | WP_001749960 | type IV secretion system protein | virB5 |
AM364_RS29030 (AM364_30180) | 42196..42423 | + | 228 | WP_001749959 | IncN-type entry exclusion lipoprotein EexN | - |
AM364_RS29035 (AM364_30185) | 42439..43479 | + | 1041 | WP_001749958 | type IV secretion system protein | virB6 |
AM364_RS29045 (AM364_30195) | 43698..44396 | + | 699 | WP_000646594 | type IV secretion system protein | virB8 |
AM364_RS29050 (AM364_30200) | 44407..45291 | + | 885 | WP_000735066 | TrbG/VirB9 family P-type conjugative transfer protein | virB9 |
AM364_RS29055 (AM364_30205) | 45291..46451 | + | 1161 | WP_000101710 | type IV secretion system protein VirB10 | virB10 |
AM364_RS29060 (AM364_30210) | 46493..47488 | + | 996 | WP_000128596 | ATPase, T2SS/T4P/T4SS family | virB11 |
AM364_RS29065 (AM364_30215) | 47488..48021 | + | 534 | WP_000792636 | phospholipase D family protein | - |
AM364_RS30640 | 48195..48482 | - | 288 | Protein_61 | DUF6710 family protein | - |
AM364_RS29075 (AM364_30225) | 48749..48928 | + | 180 | Protein_62 | helix-turn-helix domain-containing protein | - |
AM364_RS29080 (AM364_30230) | 48993..49553 | + | 561 | Protein_63 | IS6-like element IS26 family transposase | - |
AM364_RS29085 (AM364_30235) | 49573..51684 | - | 2112 | Protein_64 | Tn3-like element Tn3 family transposase | - |
Host bacterium
ID | 1401 | GenBank | NZ_CP020066 |
Plasmid name | unitig_5-pAR_0117 | Incompatibility group | IncN |
Plasmid size | 72663 bp | Coordinate of oriT [Strand] | 11416..12042 [+] |
Host baterium | Klebsiella pneumoniae strain AR_0117 |
Cargo genes
Drug resistance gene | - |
Virulence gene | - |
Metal resistance gene | - |
Degradation gene | - |
Symbiosis gene | - |
Anti-CRISPR | - |