Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100942
Name   oriT_unitig_1-pAR_0061 in_silico
Organism   Escherichia coli strain AR_0061
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NZ_CP020059 (209..835 [-], 627 nt)
oriT length   627 nt
IRs (inverted repeats)      IR1: 198..207, 211..220  (ATTGGGTGTT..AATACCTAAT)
 IR2:319..324, 330..335  (AAAAAA..TTTTTT)
  IR3: 393..402, 405..414  (GTATTCATGC..GCATGAATAC)
Location of nic site      301..302
Conserved sequence flanking the
  nic site    		  GGTGT|ATAGC
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 627 nt

>oriT_unitig_1-pAR_0061
AGCGCCGCAGATAATCTGACCGATTACCTCCTGAAACCAGGTCTATATAGGCCAAAAGTTCATCTGATACTTTTGCGGTTATTATTGGCATTCAGTCCTCACATTGTGCATTTTTTAAACAAAAAATTGGGATCTAACAAGCTGAAATCTTAGTATTACCAAAGTAATAAAGCAAACTCATTATAAAACAATGAGTTATTGGGTGTTTTTAATACCTAATTATTACCGAATATTGTTGCTATTTATTTTTTTATCTTTTAAATCAGTATGATAGCGTGATTTATCGCGCTGCGTTAGGTGTATAGCAGGTTAAGGGATAAAAAATCATCTTTTTTGGTAGGGGCGATCTACGTAGGTTAAGGACTAACTGGCTAAAAAGCGTTCAATATTCCGTATTCATGCTTGCATGAATACCAGTACAACAAAAGTACATCAAAATTACATCAAAATTACATCACTTGAAGGTTGACAGTACAACAAAATTACATCATTCTTTGGTCATGAGGTAGCCAGTACAACAAAAGTACATCAAAAGTACATCAAAATTACATCAAAATTACATCATTCTAAATGAGGGTACTATGAAGCCCAAAAGTATCAGGGCGGCACTTCAGTTGATGTTGCCGG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   1012 GenBank   WP_012561166
Name   Relaxaseofunitig_1-pAR_0061 insolico UniProt ID   _
Length   1078 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 1078 a.a.        Molecular weight: 120141.30 Da        Isoelectric Point: 6.5229

>WP_012561166.1 MULTISPECIES: MobF family relaxase [Gammaproteobacteria]
MLDITTITRQNVTSVVGYYSDAKDDYYSKDSSFTSWQGTGAEALGLSGDVESARFKELLVGEIDTFTHMQ
RHVGDAKKERLGYDLTFSAPKGVSMQALIHGDKTIIEAHEKAVAAAVREAEKLAQARTTRQGKSVTQNTN
NLVVATFRHETSRALDPDLHTHAFVMNMTQREDGQWRALKNDELMRNKMHLGDVYKQELALELTKAGYEL
RYNSKNNTFDMAHFSDEQIRAFSRRSEQIEKGLAAMGLTRETADAQTKSRVSMATREKKTEHSREEIHQE
WASRAKTLGIDFDNREWQGHGKPLEADIARNMAPDFTSPEVKADRAIQFAVKSLSERDASFERQKLIQIA
NKQVLGHATMADVEKAYLKAVQKGAIIEGEARYQSTLKVGASVMAETLTRKEWIDSLTNSGMRADKARFA
VDDGIKNGRLKKTSHRVTTVEGIRLERSILTIESRGRGQMPRQLTAEIAGQLLAGKTLKNEQMRAVTEIV
TSKDRFVAAHGYAGTGKSYMTMAAKELLESQGLKVTALAPYGTQKKALEDDGLPARTVAAFLKAKDKKLD
EKSVVFIDEAGVIPARQMKQLMEVIEKHNARAVFLGDTSQTKAVEAGKPFEQLIKAGMQTSYMKDIQRQK
NEVLLEAVKYAAEGNAARALKNITGVNELKEEAPRLAQLADRYLSLSSEQQDATLIISGTNASRKTLNDY
IRGNLGLAGTGETFTLLDRVDSTQAERRDSRYFSKGQIIIPEQDYKNGMKRGESYQVLDTGPGNKLTVES
SSGEQIAFSPRTHTKLSVYQAVSAELAPGDKVMVTRNDKTLDVANGDRFTVKTVEGEKLTLEDKKGRTVE
LDKKQASYLSYAYATTVHKSQGLTCDRVLFNIDTKSLTTSKDVFYVGISRARHEVEIFTDDKKSLASSVS
RDSPKTTAAEIDRFFGLEARFKDIGRDTSLETRSAEKGLPEATGESMAFNQKPDEHNMTTGTDYQPVSNA
EDAFHLKQNPMDDSVGLRRHEAQQNDAELAHDYAAADDQQWSAQEYADYEHYAEASDYDFDSSIYDDYAM
PQTSQAEQSHTGKEHTHEHEHEEGGHEI

  Protein domains


Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C_2)

(851-895)

TrwC relaxase (TrwC)

(16-287)

AAA domain (AAA_30)

(477-653)


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 53824..64014

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM440_RS23820 (AM440_24365) 49628..51739 + 2112 Protein_50 Tn3-like element Tn3 family transposase -
AM440_RS23825 (AM440_24370) 51759..52319 - 561 Protein_51 IS6-like element IS26 family transposase -
AM440_RS23830 (AM440_24375) 52384..52563 - 180 Protein_52 helix-turn-helix domain-containing protein -
AM440_RS25730 (AM440_24380) 52830..53117 + 288 Protein_53 DUF6710 family protein -
AM440_RS23840 (AM440_24385) 53291..53824 - 534 WP_000792636 phospholipase D family protein -
AM440_RS23845 (AM440_24390) 53824..54819 - 996 WP_000128596 ATPase, T2SS/T4P/T4SS family virB11
AM440_RS23850 (AM440_24395) 54861..56021 - 1161 WP_000101710 type IV secretion system protein VirB10 virB10
AM440_RS23855 (AM440_24400) 56021..56905 - 885 WP_000735066 TrbG/VirB9 family P-type conjugative transfer protein virB9
AM440_RS23860 (AM440_24405) 56916..57614 - 699 WP_000646594 type IV secretion system protein virB8
AM440_RS23865 (AM440_24410) 57604..57750 - 147 WP_001257173 conjugal transfer protein TraN -
AM440_RS23870 (AM440_24415) 57833..58873 - 1041 WP_001749958 type IV secretion system protein virB6
AM440_RS23875 (AM440_24420) 58889..59116 - 228 WP_001749959 IncN-type entry exclusion lipoprotein EexN -
AM440_RS23880 (AM440_24425) 59124..59837 - 714 WP_001749960 type IV secretion system protein virB5
AM440_RS23885 (AM440_24430) 59855..62323 - 2469 WP_224743086 VirB4 family type IV secretion/conjugal transfer ATPase virb4
AM440_RS23890 (AM440_24435) 62455..62772 - 318 WP_000496058 VirB3 family type IV secretion system protein virB3
AM440_RS23895 (AM440_24440) 62822..63115 - 294 WP_001749962 hypothetical protein virB2
AM440_RS23900 (AM440_24445) 63125..63406 - 282 WP_000440698 transcriptional repressor KorA -
AM440_RS23905 (AM440_24450) 63415..64014 - 600 WP_020319996 lytic transglycosylase domain-containing protein virB1
AM440_RS23910 (AM440_24455) 64258..64563 + 306 WP_000960954 H-NS family nucleoid-associated regulatory protein -
AM440_RS23915 (AM440_24460) 64579..64923 + 345 WP_001749964 hypothetical protein -
AM440_RS23920 (AM440_24465) 64920..65234 + 315 WP_001749965 TrbM/KikA/MpfK family conjugal transfer protein -
AM440_RS23925 (AM440_24470) 65270..65581 + 312 WP_001452736 hypothetical protein -
AM440_RS23930 (AM440_24475) 65631..66278 + 648 WP_012561184 restriction endonuclease -
AM440_RS23935 (AM440_24480) 66283..66489 + 207 WP_001749967 hypothetical protein -
AM440_RS23940 (AM440_24485) 66500..66772 - 273 Protein_74 IS1 family transposase -
AM440_RS23945 (AM440_24490) 66871..68304 + 1434 WP_001288432 DNA cytosine methyltransferase -

Host bacterium

ID   1400 GenBank   NZ_CP020059
Plasmid name   unitig_1-pAR_0061 Incompatibility group   IncFII
Plasmid size   87982 bp Coordinate of oriT [Strand]   209..835 [-]
Host baterium   Escherichia coli strain AR_0061

Cargo genes

Drug resistance gene   sul2, aph(3'')-Ib, aph(6)-Id, blaTEM-1A, blaOXA-9, ant(3'')-Ia, aac(6')-Ib, blaKPC-3, dfrA14
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -