Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 100941 |
| Name | oriT_unitig_2-pAR_0069  |
| Organism | Escherichia coli strain AR_0069 |
| Sequence Completeness | intact |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP020056 (98917..99467 [-], 551 nt) |
| oriT length | 551 nt |
| IRs (inverted repeats) | IR1: 62..70, 71..79 (AACCCTTTC..GACAGGGTT) IR2: 143..150, 154..161 (TGGCCTGC..GGAGGCCA) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | predicted by the oriTfinder |
oriT sequence
Download Length: 551 nt
>oriT_unitig_2-pAR_0069
GAGCAGAGCTATGTGTGACAAGAAGTATAGAAATTACGAGGTAGCCATCATGGTCGATGTGAACCCTTTCGACAGGGTTATGAATGAATTGAAAAGTCGTGGCCGCAAGAACGCTCACATCCTGAGCATCCTCCAATTCGACTGGCCTGCATCGGAGGCCATCATCGAGAAGCTGAGCTGCTACATCACAGACGGGATTAAGGCTAATCAGGAGCCTGTGATTTACCCGATCATTGAAGAAGCTCTGCATCGCTACAGCCAGCTCGTGTTTCATGAGCAGAGAGAGAAATATGAAGACCCGGCCAGAATTGGGGCATTTCTGGAAACCCTGATCACCGAAACCTGCCGGGCGTTGGAAGTGCAAATTGTCGATAGTGGCGGTGATTCATGGTCTGTCGATTCAGGAGAGTCGTTCTCACTGTGGCTTTCTTCCCATCCAGGAGAACTATCCATTAACCCGCAGCCCCATGAGGATGAGACCTCTTTGCGTGGCTTGCTGTATGAGCTCATCACCTGTGAGAGCGTGAAAACTGTTTTAAGGAGAACCGACT
GAGCAGAGCTATGTGTGACAAGAAGTATAGAAATTACGAGGTAGCCATCATGGTCGATGTGAACCCTTTCGACAGGGTTATGAATGAATTGAAAAGTCGTGGCCGCAAGAACGCTCACATCCTGAGCATCCTCCAATTCGACTGGCCTGCATCGGAGGCCATCATCGAGAAGCTGAGCTGCTACATCACAGACGGGATTAAGGCTAATCAGGAGCCTGTGATTTACCCGATCATTGAAGAAGCTCTGCATCGCTACAGCCAGCTCGTGTTTCATGAGCAGAGAGAGAAATATGAAGACCCGGCCAGAATTGGGGCATTTCTGGAAACCCTGATCACCGAAACCTGCCGGGCGTTGGAAGTGCAAATTGTCGATAGTGGCGGTGATTCATGGTCTGTCGATTCAGGAGAGTCGTTCTCACTGTGGCTTTCTTCCCATCCAGGAGAACTATCCATTAACCCGCAGCCCCATGAGGATGAGACCTCTTTGCGTGGCTTGCTGTATGAGCTCATCACCTGTGAGAGCGTGAAAACTGTTTTAAGGAGAACCGACT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 1011 | GenBank | WP_001326173 |
| Name | TraIofunitig_2-pAR_0069 |
UniProt ID | _ |
| Length | 990 a.a. | PDB ID | |
| Note | putative relaxase | ||
Relaxase protein sequence
Download Length: 990 a.a. Molecular weight: 109356.90 Da Isoelectric Point: 4.9395
>WP_001326173.1 MULTISPECIES: MobH family relaxase [Gammaproteobacteria]
MLKALNKLFGGRSGVIETAPSVRVLPLKDVEDEEIPRYPPFAKGLPVAPLDKILATQAELIEKVRNSLGF
TVDDFNRLVLPVIQRYAAFVHLLPASESHHHRGAGGLFRHGLEVAFWAAQASESVIFSIEGTPRERRDNE
PRWRLASCFSGLLHDVGKPLSDVSITDKDGSITWNPYSESLHDWAHRHEIDRYFIRWRDKRHKRHEQFSL
LAVDRIIPAETREFLSKSGPSIMEAMLEAISGTSVNQPVTKLMLRADQESVSRDLRQSRLDVDEFSYGVP
VERYVFDAIRRLVKTGKWKVNEPGAKVWHLNQGVFIAWKQLGDLYDLISHDKIPGIPRDPDTLADILIER
GFAVPNTVQEKGERAYYRYWEVLPEMLQEAAGSVKILMLRLESNDLVFTTEPPAAVAAEVVGDVEDAEIE
FVDPEEVDDDQEEDVSALNDDMLAAEQEAEKALAGLGFGDAMEMLKSTSDAVEEKPEQKDAGSTESSKPD
AGKKGKPQSKPGKAKPKSDTEKQPHKPEAKEDLSPQDIAKNAPPLANDNPLQALKDVGGGLGDIDFPFDA
FSASAETASTDATNSEIPDVAMPGKQEKQPKQDFVPQEQNSLQGDDFPMFGSSDEPPSWAIEPLPMLTDA
PEQTTPAPAMPPTDKPNLHEKDAKTLLVEMLAGYGEASALLEQAIMPVLEGKTTLGEVLCLMKGQAVILY
PDGARSLGAPSEVLSKLSHANAIVPDPIMPGRKVRDFSGVKAIVLAEQLSDAVVAAIKDAEASMGGYQDA
FELVSPPGLDASKNKSAPKQQSRKKAQQQKPEVNAGKPSPEQKAKGKDSQPQQKEKKVDVTSPVEEPQRQ
PVQEKQNVARLPKREVQPVAPEPKVEREKELGHVEVREREEPEVREFEPPKAKTNPKDINAEDFLPSGVT
PQKALQMLKDMIQKRSGRWLVTPVLEEDGCLVTSDKAFDMIAGENIGISKHILCGMLSRAQRRPLLKKRQ
GKLYLEVNET
MLKALNKLFGGRSGVIETAPSVRVLPLKDVEDEEIPRYPPFAKGLPVAPLDKILATQAELIEKVRNSLGF
TVDDFNRLVLPVIQRYAAFVHLLPASESHHHRGAGGLFRHGLEVAFWAAQASESVIFSIEGTPRERRDNE
PRWRLASCFSGLLHDVGKPLSDVSITDKDGSITWNPYSESLHDWAHRHEIDRYFIRWRDKRHKRHEQFSL
LAVDRIIPAETREFLSKSGPSIMEAMLEAISGTSVNQPVTKLMLRADQESVSRDLRQSRLDVDEFSYGVP
VERYVFDAIRRLVKTGKWKVNEPGAKVWHLNQGVFIAWKQLGDLYDLISHDKIPGIPRDPDTLADILIER
GFAVPNTVQEKGERAYYRYWEVLPEMLQEAAGSVKILMLRLESNDLVFTTEPPAAVAAEVVGDVEDAEIE
FVDPEEVDDDQEEDVSALNDDMLAAEQEAEKALAGLGFGDAMEMLKSTSDAVEEKPEQKDAGSTESSKPD
AGKKGKPQSKPGKAKPKSDTEKQPHKPEAKEDLSPQDIAKNAPPLANDNPLQALKDVGGGLGDIDFPFDA
FSASAETASTDATNSEIPDVAMPGKQEKQPKQDFVPQEQNSLQGDDFPMFGSSDEPPSWAIEPLPMLTDA
PEQTTPAPAMPPTDKPNLHEKDAKTLLVEMLAGYGEASALLEQAIMPVLEGKTTLGEVLCLMKGQAVILY
PDGARSLGAPSEVLSKLSHANAIVPDPIMPGRKVRDFSGVKAIVLAEQLSDAVVAAIKDAEASMGGYQDA
FELVSPPGLDASKNKSAPKQQSRKKAQQQKPEVNAGKPSPEQKAKGKDSQPQQKEKKVDVTSPVEEPQRQ
PVQEKQNVARLPKREVQPVAPEPKVEREKELGHVEVREREEPEVREFEPPKAKTNPKDINAEDFLPSGVT
PQKALQMLKDMIQKRSGRWLVTPVLEEDGCLVTSDKAFDMIAGENIGISKHILCGMLSRAQRRPLLKKRQ
GKLYLEVNET
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 92243..101333
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| AM448_RS26275 (AM448_24730) | 87268..87633 | - | 366 | Protein_91 | LuxR family transcriptional regulator | - |
| AM448_RS24395 (AM448_24735) | 87890..88207 | + | 318 | WP_000118520 | quaternary ammonium compound efflux SMR transporter SugE | - |
| AM448_RS24400 (AM448_24740) | 88204..88737 | - | 534 | WP_001221666 | lipocalin family protein | - |
| AM448_RS24405 (AM448_24745) | 88831..89976 | - | 1146 | WP_015058212 | class C beta-lactamase CMY-6 | - |
| AM448_RS24410 (AM448_24755) | 90300..91562 | - | 1263 | WP_000608644 | IS1380-like element ISEcp1 family transposase | - |
| AM448_RS24415 (AM448_24760) | 91847..92239 | - | 393 | WP_000479535 | TraA family conjugative transfer protein | - |
| AM448_RS24420 (AM448_24765) | 92243..92821 | - | 579 | WP_000793435 | type IV conjugative transfer system lipoprotein TraV | traV |
| AM448_RS24425 (AM448_24770) | 92818..94131 | - | 1314 | WP_024131605 | TraB/VirB10 family protein | traB |
| AM448_RS24430 (AM448_24775) | 94131..95048 | - | 918 | WP_000794249 | type-F conjugative transfer system secretin TraK | traK |
| AM448_RS24435 (AM448_24780) | 95032..95658 | - | 627 | WP_001049717 | TraE/TraK family type IV conjugative transfer system protein | traE |
| AM448_RS24440 (AM448_24785) | 95655..95936 | - | 282 | WP_000805625 | type IV conjugative transfer system protein TraL | traL |
| AM448_RS24445 (AM448_24790) | 96081..96446 | - | 366 | WP_001052530 | hypothetical protein | - |
| AM448_RS24450 (AM448_24795) | 96782..97444 | - | 663 | WP_001231464 | hypothetical protein | - |
| AM448_RS24455 (AM448_24800) | 97444..97821 | - | 378 | WP_000869297 | hypothetical protein | - |
| AM448_RS24460 (AM448_24805) | 97831..98277 | - | 447 | WP_000122507 | hypothetical protein | - |
| AM448_RS24465 (AM448_24810) | 98287..98916 | - | 630 | WP_000743449 | DUF4400 domain-containing protein | tfc7 |
| AM448_RS24470 (AM448_24815) | 98873..99418 | - | 546 | WP_000228720 | hypothetical protein | - |
| AM448_RS24475 (AM448_24820) | 99468..101333 | - | 1866 | WP_000178857 | conjugative transfer system coupling protein TraD | virb4 |
| AM448_RS24480 (AM448_24825) | 101330..104302 | - | 2973 | WP_001326173 | MobH family relaxase | - |
| AM448_RS24485 (AM448_24830) | 104470..105087 | + | 618 | WP_001249395 | hypothetical protein | - |
| AM448_RS24490 (AM448_24835) | 105069..105302 | + | 234 | WP_001191890 | hypothetical protein | - |
Host bacterium
| ID | 1399 | GenBank | NZ_CP020056 |
| Plasmid name | unitig_2-pAR_0069 | Incompatibility group | IncA/C2 |
| Plasmid size | 136975 bp | Coordinate of oriT [Strand] | 98917..99467 [-] |
| Host baterium | Escherichia coli strain AR_0069 |
Cargo genes
| Drug resistance gene | blaNDM-1, sul1, qacE, aac(6')-Ib, blaCMY-6 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |