Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100939
Name   oriT_pGeo12b in_silico
Organism   Geobacillus thermodenitrificans strain T12
Sequence Completeness      core
NCBI accession of oriT (coordinates [strand])   NZ_CP020032 (1922..1955 [-], 34 nt)
oriT length   34 nt
IRs (inverted repeats)      4..18, 22..34  (CCCATTTATACCCCT..AGGGGTAAATGGG)
Location of nic site      2..3nt
Conserved sequence flanking the
  nic site    		  _
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 34 nt

>oriT_pGeo12b
GTGCCCATTTATACCCCTTTCAGGGGTAAATGGG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   1009 GenBank   WP_087960466
Name   Nickase_pGeo12b insolico UniProt ID   _
Length   629 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 629 a.a.        Molecular weight: 73916.06 Da        Isoelectric Point: 9.4322

>WP_087960466.1 MobQ family relaxase [Geobacillus thermodenitrificans]
MTMAYFLFRTQIISKSNRSSVACAAYRSGEALYSERDGLTKKYGHRDIPPETYILAPKHAPDWVYNRERL
WNEVEKVEKQHNAQLAREIIVALPTQLTNEEQTQLLLDFCKENFSDEGMVADIAIHRDKKHNPHAHIMLT
MRPFNEDGTWGQKRKKVNGKSVHLTNWNDKETLLKWRKNFADRINEKFKEKGINDRVSHESYEKQGIDKV
PQIRLSREAYQYEQRMKREAEKQGKPYEPVTYYGKLNQEIQRINQELKALKQQEKVVSISEYQEEKSLNE
TIESIRKNASLNDVEKASLLLVAKRAKSYVDFAVARRVYHDLAEGNWKKKIDSQQLKLRALKNVINKAYH
VYQQEPKQVIQYGFHPGKFNEQLREKIAQLKEMEKSFENELTKYEAVLKKAELALEVQKRLTEEEFRVLY
PHAGADFRIEEKHHAVQYFKDTGGVLPESEIPAYAAKKEQEIHRMPTFAEQTRNMYQSLFVLNRTIQKQS
KERMEALKARDFEQAFEASRKIEQCMLRKEKMSKELEENKAVLQAILSEHYGRDVSITNTAALLRLHELV
ERGESSKDFEADLRRIHVSEQQKLERMPKPETEAQIMEYQYRQHVADGLLEAIEEAVKNLNAKGERKSR

  Protein domains


Predicted by InterproScan.

MobA/MobL family (MobA_MobL)

(18-225)


  Protein structure



No available structure.




Host bacterium

ID   1397 GenBank   NZ_CP020032
Plasmid name   pGeo12b Incompatibility group   _
Plasmid size   56976 bp Coordinate of oriT [Strand]   1922..1955 [-]
Host baterium   Geobacillus thermodenitrificans strain T12

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -