Detailed information of oriT

oriT


The information of the oriT region


oriTDB ID   100936
Name   oriT_pASM4 in_silico
Organism   Enterobacter roggenkampii strain R11
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NZ_CP019843 (679..852 [-], 174 nt)
oriT length   174 nt
IRs (inverted repeats)      2..11, 15..24  (GTTTCGGGGC..GCCCTGAACC)
Location of nic site      55..56
Conserved sequence flanking the
  nic site  
 
 CTGG|CTTA
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 174 nt

>oriT_pASM4
GGTTTCGGGGCGCAGCCCTGAACCAGTCACGTAGCGCTAGCGGAGTGTATACTGGCTTACTATGTTGGCACGGATGCGAGTGTCAGTGAAATGCTTCATGTGGCAGGAGAAAAATGCAGCAGCGGAGCGTCAGCAGAATATGTGATACAAGATATATTCCGCTTCCTCGCTCAC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase


ID   1006 GenBank   WP_012565229
Name   MobA_pASM4 insolico UniProt ID   _
Length   499 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 499 a.a.        Molecular weight: 55380.75 Da        Isoelectric Point: 8.2087

>WP_012565229.1 relaxase/mobilization nuclease domain-containing protein [Enterobacter roggenkampii]
MIVKFHPRGRGGGAGPVDYLLGKDRQREGATVLQGKPEEVRELIDASPYVKKYTSGVLSFAEADLPSGQR
EKLMASFERVLMPGLDKDQYSILWVEHRDKGRLELNFLIPNTELLTGRRLQPYYDRADRPRIDAWQTIVN
GRLGLHDPNAPENRRALVTPSALPKAKQEAAEAITRGLLALASSGELKTRQDVTEALESAGFEVVRTTKS
SISIADPDGGRNIRLKGAIYEQSFNAGEGLRAEIESAAAEYRRDAESRIQRAREICKSGTERKREENQRR
HPRPRPETVLSHEPAHERHGAHGQPDVADHRPGLRAADRLERGHSVVAGAADTRQLHDHPGAEEHAGHAV
REEQRRAALDLRRAETPLREGEPGSGTVRRGFELDDTGGEIAHDGAGKTVAERIRAATAGLLEKAGRVGE
RLRGMADDVWSYATGERSAERARHGLEQAGAEFERAAAPVVERLNAVELHREQERVVQHQKALELERSQR
QRTYNSPSL

  Protein domains


Predicted by InterproScan.

(58-238)


  Protein structure



No available structure.




Host bacterium


ID   1394 GenBank   NZ_CP019843
Plasmid name   pASM4 Incompatibility group   ColRNAI
Plasmid size   4667 bp Coordinate of oriT [Strand]   679..852 [-]
Host baterium   Enterobacter roggenkampii strain R11

Cargo genes


Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -