Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100934
Name   oriT1_pASM3 in_silico
Organism   Enterobacter roggenkampii strain R11
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NZ_CP019842 ( 7034..7207 [-], 174 nt)
oriT length   174 nt
IRs (inverted repeats)      2..11, 15..24  (GTTTCGGGGC..GCCCTGAACC)
Location of nic site      55..56
Conserved sequence flanking the
  nic site    		  CTGG|CTTA
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 174 nt

>oriT1_pASM3
GGTTTCGGGGCGCAGCCCTGAACCAGTCACGTAGCGCTAGCGGAGTGTATACTGGCTTACTATATTGGCACGGATGCGAATATCAGTGAAGTGCTTCATCTGGCAGGAGAAAAATGCAGCAGCGGAGCGTCAGCAGAATATGTGATACAGGATATATTCCGCTTCCTCGCTCAC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   1005 GenBank   WP_045282379
Name   PutativerelaxaseofpASM3 insolico UniProt ID   _
Length   501 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 501 a.a.        Molecular weight: 55306.65 Da        Isoelectric Point: 7.6225

>WP_045282379.1 MULTISPECIES: relaxase/mobilization nuclease domain-containing protein [Gammaproteobacteria]
MIVKFHPRGRGGGAGPVDYLLGKDRQREGASVLQGKPEEVRELIDASPYVKKYTSGVLSFAEADLPPGQR
EKLMASFERVLMPGLDKDQYSILWVEHEDKGRLELNFLIPNTELLTGRRLQPYYDRADRPRIDAWQTIVN
GRLGLHDPNAPENRRALVTPAGLPKTKQEAAEAITRGLLSLASSGELKSRQDVTEALEGAGFEVVCTTKS
SISIADPDGGRNIRLKGAIYEQSFNAGEGLRAAIESAAEEYRRDAESRIQRARAVCQSGTERKREENQRR
HQRPRAEYERGDGEKPAERVADGREDMAHHPDGVSAADRHEREHSVVAGAADNRQLHASSSAGRYPGEND
RPDVGSALSGEQRRPEVSYPAAGDASRSHPLRRDDMDPPETGVAENDRAGKAVAERIRAATAGLLAKAGR
MGERLRGMAEHVWSYATGERGAERARVGLEHAGAEFKRAAAPLVRELNAIEEKAALEYQRQNELELTLRQ
KQKSFDGPKMR

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(57-238)


  Protein structure



No available structure.




Host bacterium

ID   1393 GenBank   NZ_CP019842
Plasmid name   pASM3 Incompatibility group   Col440II
Plasmid size   11748 bp Coordinate of oriT [Strand]   222..395 [-]; 7034..7207 [-]
Host baterium   Enterobacter roggenkampii strain R11

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -