Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100921
Name   oriT_pECAZ159_2 in_silico
Organism   Escherichia coli strain Ecol_AZ159
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NZ_CP019006 (327..953 [+], 627 nt)
oriT length   627 nt
IRs (inverted repeats)      IR1: 198..207, 211..220  (ATTGGGTGTT..AATACCTAAT)
 IR2:319..324, 330..335  (AAAAAA..TTTTTT)
  IR3: 393..402, 405..414  (GTATTCATGC..GCATGAATAC)
Location of nic site      301..302
Conserved sequence flanking the
  nic site    		  GGTGT|ATAGC
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 627 nt

>oriT_pECAZ159_2
AGCGCCGCAGATAATCTGACCGATTACCTCCTGAAACCAGGTCTATATAGGCCAAAAGTTCATCTGATACTTTTGCGGTTATTATTGGCATTCAGTCCTCACATTGTGCATTTTTTAAACAAAAAATTGGGATCTAACAAGCTGAAATCTTAGTATTACCAAAGTAATAAAGCAAACTCATTATAAAACAATGAGTTATTGGGTGTTTTTAATACCTAATTATTACCGAATATTGTTGCTATTTATTTTTTTATCTTTTAAATCAGTATGATAGCGTGATTTATCGCGCTGCGTTAGGTGTATAGCAGGTTAAGGGATAAAAAATCATCTTTTTTGGTAGGGGCGATCTACGTAGGTTAAGGACTAACTGGCTAAAAAGCGTTCAATATTCCGTATTCATGCTTGCATGAATACCAGTACAACAAAAGTACATCAAAATTACATCAAAATTACATCACTTGAAGGTTGACAGTACAACAAAATTACATCATTCTTTGGTCATGAGGTAGCCAGTACAACAAAAGTACATCAAAAGTACATCAAAATTACATCAAAATTACATCATTCTAAATGAGGGTACTATGAAGCCCAAAAGTATCAGGGCGGCACTTCAGTTGATGTTGCCGG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   992 GenBank   WP_001749977
Name   Relaxase_pECAZ159_2 insolico UniProt ID   _
Length   1080 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 1080 a.a.        Molecular weight: 120419.59 Da        Isoelectric Point: 6.5483

>WP_001749977.1 MULTISPECIES: MobF family relaxase [Gammaproteobacteria]
MLDITTITRQNVTSVVGYYSDAKDDYYSKDSSFTSWQGTGAEALGLSGDVESARFKELLVGEIDTFTHMQ
RHVGDAKKERLGYDLTFSAPKGVSMQALIHGDKTIIEAHEKAVAAAVREAEKLAQARTTRQGKSVTQNTN
NLVVATFRHETSRALDPDLHTHAFVMNMTQREDGQWRALKNDELMRNKMHLGDVYKQELALELTKAGYEL
RYNSKNNTFDMAHFSDEQIRAFSRRSEQIEKGLAAMGLTRETADAQTKSRVSMATREKKTEHSREEIHQE
WASRAKTLGIDFDNREWQGHGKPLEADIARNMAPDFTSPEVKADRAIQFAVKSLSERDASFERQKLIQIA
NKQVLGHATIADVEKAYLKAVQKGAIIEGEARYQSTLKVGASVMAETLTRKEWIDSLTNSGMRADKARFA
VDDGIKNGRLKKTSHRVTTVEGIRLERSILTIESRGRGQMPRQLTAEIAGQLLAGKTLKKEQMRAVTEIV
TSKDRFVAAHGYAGTGKSYMTMAAKELLESQGLKVTALAPYGTQKKALEDDGLPARTVAAFLKAKDKKLD
EKSVVFIDEAGVIPARQMKQLMEVIEKHNARAVFLGDTSQTKAVEAGKPFEQLIKAGMQTSYMKDIQRQK
NEVLLEAVKYAAEGNAARALKNITGVNELKEEAPRLSQLADRYLSLSSEQQDATLIISGTNASRKTLNDY
IRGNLGLAGTGETFTLLDRVDSTQAERRDSRYFSKGQIIIPEQDYKNGMKRGESYQVLDTGPGNKLTVES
SSGEQIAFSPRTHTKLSVYQAVSAELAPGDKVMVTRNDKTLDVANGDRFTVKTVEGEKLTLEDKKGRTVE
LDKKQASYLSYAYATTVHKSQGLTCDRVLFNIDTKSLTTSKDVFYVGISRARHEVEIFTDDKKSLASSVS
RDSPKTTAAEIDRFFGLEARFKDIGRDTSLETRSAEKGLPEATGESMAFNQKPDEHNMTTGTDYQPVSNA
EDAFHLKQNPMDDSVGLRRHEAQQNDAELAHDYAAADDQQWSAQEYADYEHYAEASDYDFDSSIYDDYAM
PQTSQAEQSHTGKEHTHEHEHEHEEGGHEI

  Protein domains


Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C_2)

(851-895)

TrwC relaxase (TrwC)

(16-287)

AAA domain (AAA_30)

(477-653)


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 24949..35274

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BE939_RS00170 (BE939_00165) 20794..22227 - 1434 WP_001288432 DNA cytosine methyltransferase -
BE939_RS00175 (BE939_00170) 22326..22598 + 273 Protein_29 IS1 family transposase -
BE939_RS00180 (BE939_00175) 22609..22815 - 207 WP_001749967 hypothetical protein -
BE939_RS00185 (BE939_00180) 22820..23467 - 648 WP_015344958 restriction endonuclease -
BE939_RS00190 (BE939_00185) 23517..23828 - 312 WP_001452736 hypothetical protein -
BE939_RS00195 (BE939_00190) 23864..24178 - 315 WP_001749965 TrbM/KikA/MpfK family conjugal transfer protein -
BE939_RS00200 (BE939_00195) 24175..24519 - 345 WP_001749964 hypothetical protein -
BE939_RS00205 (BE939_00200) 24535..24906 - 372 WP_011867773 H-NS family nucleoid-associated regulatory protein -
BE939_RS00210 (BE939_00205) 24949..25683 + 735 WP_001749963 lytic transglycosylase domain-containing protein virB1
BE939_RS00215 (BE939_00210) 25692..25973 + 282 WP_000440698 transcriptional repressor KorA -
BE939_RS00220 (BE939_00215) 25983..26276 + 294 WP_001749962 hypothetical protein virB2
BE939_RS00225 (BE939_00220) 26326..26643 + 318 WP_000496058 VirB3 family type IV secretion system protein virB3
BE939_RS00230 (BE939_00225) 26643..29243 + 2601 WP_001749961 VirB4 family type IV secretion/conjugal transfer ATPase virb4
BE939_RS00235 (BE939_00230) 29261..29974 + 714 WP_001749960 type IV secretion system protein virB5
BE939_RS00240 (BE939_00235) 29982..30209 + 228 WP_001749959 IncN-type entry exclusion lipoprotein EexN -
BE939_RS00245 (BE939_00240) 30225..31265 + 1041 WP_001749958 type IV secretion system protein virB6
BE939_RS00250 (BE939_00245) 31348..31494 + 147 WP_001257173 conjugal transfer protein TraN -
BE939_RS00255 (BE939_00250) 31484..32182 + 699 WP_000646594 type IV secretion system protein virB8
BE939_RS00260 (BE939_00255) 32193..33077 + 885 WP_000735066 TrbG/VirB9 family P-type conjugative transfer protein virB9
BE939_RS00265 (BE939_00260) 33077..34237 + 1161 WP_000101710 type IV secretion system protein VirB10 virB10
BE939_RS00270 (BE939_00265) 34279..35274 + 996 WP_000128596 ATPase, T2SS/T4P/T4SS family virB11
BE939_RS00275 (BE939_00270) 35274..35519 + 246 Protein_49 phospholipase D-like domain-containing protein -
BE939_RS00280 (BE939_00275) 36000..36311 + 312 Protein_50 transposase -
BE939_RS00285 (BE939_00280) 36561..37442 - 882 WP_004199234 carbapenem-hydrolyzing class A beta-lactamase KPC-2 -
BE939_RS00290 (BE939_00285) 37664..38314 - 651 WP_045286946 class A beta-lactamase -
BE939_RS00295 (BE939_00290) 38406..38963 - 558 WP_001217881 recombinase family protein -

Host bacterium

ID   1380 GenBank   NZ_CP019006
Plasmid name   pECAZ159_2 Incompatibility group   IncN
Plasmid size   47531 bp Coordinate of oriT [Strand]   327..953 [+]
Host baterium   Escherichia coli strain Ecol_AZ159

Cargo genes

Drug resistance gene   aac(6')-Ib-cr, ARR-3, dfrA27, aadA16, qacE, sul1, blaKPC-2
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -