Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100918
Name   oriT_pECAZ146_3 in_silico
Organism   Escherichia coli strain Ecol_AZ146
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NZ_CP018988 (15601..15955 [+], 355 nt)
oriT length   355 nt
IRs (inverted repeats)      IR1: 145..155, 161..171  (ATGTGTAATAC..GTATTACACAT)
  IR2: 208..218, 223..233  (GCATTTACGAT..ATCGTAATTGC)
  IR3: 336..344, 347..355  (CTTTCATGC..GCATGAAAG)
Location of nic site      242..243
Conserved sequence flanking the
  nic site    		  GGTGT|ATAGC
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 355 nt

>oriT_pECAZ146_3
ACTCCGATACGTTGCCCTCCGAACGGTATTCAAGGTCGATTTTTTGCGCTTCGGTCACTCTTAAACTGATAGATGGCATAGGTTTCCTTTGTGTAATACCGATGTAATACATTCAAATCTAGCATATATGCGGCTTAATTCCACATGTGTAATACGCTGTGTATTACACATTAAAACACAAATTAGAATAATTTGTTTTGTTTTCAAGCATTTACGATGAAAATCGTAATTGCGTATGGTGTATAGCCGTTAAGGGATACCATATCACGCCTTTTTTAAGGGAGAAACCGGTGTTACGTGCAAGTGAATCGCTCAAAAAGCGTTCACATTCACCACTTTCATGCTCGCATGAAAG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   989 GenBank   WP_023187071
Name   Relaxase_pECAZ146_3 insolico UniProt ID   A0A1U9TLW3
Length   969 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 969 a.a.        Molecular weight: 108499.54 Da        Isoelectric Point: 9.7610

>WP_023187071.1 MULTISPECIES: MobF family relaxase [Enterobacterales]
MLNITTITRQRVDKVVDYYADGADDYYAKDGNAMQWQGAGAEELGLTGEVEQKRFAKLLDGKVTDDISVM
RKTANSESKERLGYDLTFSAPKGVTLQALVNGDKRIIEAHDRAVTKAIEEAERLALGRFTVNKKTHVENT
NKLIVGKFRHETSRELDPDLHTHAFVMNLTKTSDGKWRSLTNDGIVNSLTLLGNVYKSELARELELAGYN
LRYDRNGTFDLAHFSQEQIAQFSQRSQQIEAELAKNGLSRETASHEQKNAAALKTRKSKGEVDRDVLYEG
WKNRAKELQIDFDSREWKGAANDDIARNTQPAALEKPIEYQADKVMTFAIRSLTERQSVITERELMDVAM
KHGYGRLTLDDIRAARERAITSGNLIKEEATYAAATTNKKQQNVAMTREQWVTELVKAGRNKDEARKLVD
KGITNGRLVQQKPRFTTQLAQQRERNILKMEREGRGKIQTPYTREFSEGWLASRTLKPEQLKAVMGIIHT
PNQFISVHGFAGTGKSYMTKSAADFLKEQGVHVTSLAPYGSQVKALQAEGLESRTLQSFLRASDKKIGPG
SVVFIDEAGVIPARQMEETMRVIRDAGARAVFLGDTKQTKAVEAGKPFEQLIKAGMETAYMKDIQRQKDP
ELLKAALNAAEGKIKASLTHVTSIVEEKNHDQRYRRIVGDYVAMPPTDRANALIITGTNDSRKKINEYIQ
AELGLKGQGINYPLLNRLDTTQAQRQHSKYYEKGAVIIPERDYSNGLKRGAVYTVLDTGPGNKLTVSDGS
GDTIAFSPARFSKLSVYSVEKTELAVGDQVRITRNDAHLDLANGDRFKVKGVQSGEVLLENEKGRLVKID
ANKPMYLGLAYASTVHSAQGLTCDKVFINMDTRSLTTAKDVYYVAVTRAKHEAVIYTDDEKKLDKAASRE
GFKTAALELEQLKRYAKEQQHDAREKGRDKTEPTQEKDNSKRKGKGHDKNNDERAFTSL

  Protein domains


Predicted by InterproScan.

AAA domain (AAA_30)

(475-649)

UvrD-like helicase C-terminal domain (UvrD_C_2)

(850-896)

TrwC relaxase (TrwC)

(14-287)


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 1..15339

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BE932_RS00190 (BE932_00180) 1..291 + 291 WP_021536806 TrbC/VirB2 family protein virB2
BE932_RS00195 (BE932_00185) 342..656 + 315 WP_001053888 VirB3 family type IV secretion system protein virB3
BE932_RS00200 (BE932_00190) 956..3286 + 2331 WP_349496259 VirB4 family type IV secretion/conjugal transfer ATPase -
BE932_RS00205 (BE932_00195) 3296..4003 + 708 WP_023187073 type IV secretion system protein virB5
BE932_RS00210 (BE932_00200) 4007..4243 + 237 WP_000733633 EexN family lipoprotein -
BE932_RS00215 (BE932_00205) 4298..5305 + 1008 WP_172801674 type IV secretion system protein virB6
BE932_RS31100 5400..5570 + 171 WP_021536809 hypothetical protein -
BE932_RS00220 (BE932_00210) 5573..6289 + 717 WP_000914645 type IV secretion system protein virB8
BE932_RS00225 (BE932_00215) 6292..7179 + 888 WP_000744355 TrbG/VirB9 family P-type conjugative transfer protein virB9
BE932_RS00230 (BE932_00220) 7176..8330 + 1155 WP_078175471 type IV secretion system protein VirB10 virB10
BE932_RS00235 (BE932_00225) 8323..9354 + 1032 WP_078175474 P-type DNA transfer ATPase VirB11 virB11
BE932_RS00240 (BE932_00230) 9317..9871 + 555 WP_000616063 phospholipase D family protein -
BE932_RS00245 (BE932_00235) 9884..10366 + 483 WP_021536813 GNAT family protein -
BE932_RS00250 (BE932_00240) 10378..10572 + 195 WP_000529710 hypothetical protein -
BE932_RS00255 (BE932_00245) 10850..13759 - 2910 WP_023187071 MobF family relaxase -
BE932_RS00260 (BE932_00250) 13762..15339 - 1578 WP_021536815 type IV secretion system DNA-binding domain-containing protein virb4
BE932_RS00265 (BE932_00255) 15332..15679 - 348 WP_001139329 hypothetical protein -
BE932_RS00270 (BE932_00260) 16155..16589 + 435 WP_023223528 hypothetical protein -
BE932_RS00275 (BE932_00265) 16602..17312 + 711 WP_021544361 StbB family protein -
BE932_RS00280 (BE932_00270) 17314..17682 + 369 WP_015345231 plasmid stabilization protein StbC -
BE932_RS00285 (BE932_00275) 17897..18667 + 771 WP_021536818 SprT-like domain-containing protein -
BE932_RS00290 (BE932_00280) 18708..18896 - 189 WP_001001010 hypothetical protein -
BE932_RS00295 (BE932_00285) 18901..19158 - 258 WP_021536820 hypothetical protein -
BE932_RS30530 19231..19386 - 156 WP_021536821 hypothetical protein -
BE932_RS00300 (BE932_00290) 19539..19781 - 243 WP_000526841 hypothetical protein -
BE932_RS00305 (BE932_00295) 19836..20123 - 288 WP_001098466 hypothetical protein -

Host bacterium

ID   1377 GenBank   NZ_CP018988
Plasmid name   pECAZ146_3 Incompatibility group   _
Plasmid size   34532 bp Coordinate of oriT [Strand]   15601..15955 [+]
Host baterium   Escherichia coli strain Ecol_AZ146

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -