Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100917
Name   oriT_pECAZ146_4 in_silico
Organism   Escherichia coli strain Ecol_AZ146
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NZ_CP018987 (6252..6334 [-], 83 nt)
oriT length   83 nt
IRs (inverted repeats)      3..12, 16..25  (GTGTCGGGGC..GCCATGACCC)
Location of nic site      56..57
Conserved sequence flanking the
  nic site    		  CTGG|CTTA
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 83 nt

>oriT_pECAZ146_4
GGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAATCATTTGGCATCAGTGAGGATT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   988 GenBank   WP_053892877
Name   PutativerelaxaseofpECAZ146_4 insolico UniProt ID   A0A1U9TM64
Length   532 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 532 a.a.        Molecular weight: 59158.64 Da        Isoelectric Point: 8.3326

>WP_053892877.1 relaxase/mobilization nuclease domain-containing protein [Escherichia coli]
MIVKFHARGKGGGSGPVDYLLGRERNREGARVLRGAPEEVRELIDATPFAKKYTSGVLSFAEQTLPPGER
ERVMESFERVLMPGLEKNQYSILWVEHQDKGRLELNFVIPNMELASGKRLQPYYDRADRPRINAWQTLVN
HHYGLHDPNAPENRRTLVTPNNLPKAKQEAAEAITRGLEALYHAGALKTRQDVTEALTAAGFEVVRTTRS
SISIADPEGGRNLRLKGALYEQSFTNGDRLREETERACRLYREHAEQRVQEARSVCRQGTELKRAENQRR
YQRTHSSGERTTGDAEQCRERAVQRHRERTGPAAGRAALEAGQRADTVPVADGALRHGRDFSGVMGADSV
AGKPDCGEHPGYQGPGRDAGETPGKNMGCDVSRGQEREISSVAVRGEGGKQLDGGEEERSKTGQGVSEYD
RAGNALTERLRAIAAGLYAAAERLGERLHGFAAYVRADTTGERATEGTVRRLESAGAEISRAGGPLESVI
YHEQQIRDEQERQARLERERARSWHEEQEREARVWRGPSIGM

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(58-239)


  Protein structure



No available structure.




Host bacterium

ID   1376 GenBank   NZ_CP018987
Plasmid name   pECAZ146_4 Incompatibility group   ColRNAI
Plasmid size   6994 bp Coordinate of oriT [Strand]   6252..6334 [-]
Host baterium   Escherichia coli strain Ecol_AZ146

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -