Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100915
Name   oriT_pEC867_KPC in_silico
Organism   Escherichia coli strain Ecol_867
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NZ_CP018981 (7645..7823 [-], 179 nt)
oriT length   179 nt
IRs (inverted repeats)      8..15, 19..26  (TTCGGGGC..GCCCTGAA)
Location of nic site      59..60
Conserved sequence flanking the
  nic site    		  CTGG|CTTA
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 179 nt

>oriT_pEC867_KPC
AGCGGGTTTCGGGGCGCAGCCCTGAACCAGTCACGTAGCGCTAGCGGAGTGTATACTGGCTTAACATGGTAAATCGGAGTGGGGATTCAGGAAAGTGCTTCATGTGGCAGGGAGAAAATGCTGCTGTGGGGCGTAAGTGCGAAATGCAGATCCCGGTGAAATGACGCCTCCTCGCTCAC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   986 GenBank   WP_034169352
Name   PutativerelaxaseofpEC867_KPC insolico UniProt ID   _
Length   498 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 498 a.a.        Molecular weight: 55176.27 Da        Isoelectric Point: 6.9863

>WP_034169352.1 relaxase/mobilization nuclease domain-containing protein [Salmonella enterica]
MIVKFHPRGRGGGGGPVDYLLGKDRQRDGASVLQGKPEEVRELIDASPYAKKYTSGVLSFAEQDLPPGQR
EKLMASFERVLMPGLDKDQYSVLWVEHRDKGRLELNFLIPNTELLTGRRLQPYYDRADRPRIDAWQTIVN
GRLGLHDPNAPENRRALVTPSALPEAKQEAAQAITRGLLSLASSGELKTRQDVTEALESAGFEVVRTTKS
SISIADPDGGRNIRLKGAIYEQSFNAGEGLRAEIESAAADYRRDAESRIQRAREVCQSGTERKREENQRR
HPRPRPEAVLSHEPTHERDAAYDQPDVADHSPGLRATDRLERGHSVVAGATDTRQLHDHPGTEEHAGHAF
REEQRRPAHDLRGGETPLREGEPGSGKIRRGFELDDTGGEIAHDGTGKTVAERIRAATAGLLEKAGRVGE
RLRGMADDVWAYATGERGAERARHGLEQAGAEFERSAAPIVARLNGIEAHREQERAAQHQKALELERSQR
QKGFELGM

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(57-238)


  Protein structure



No available structure.




Host bacterium

ID   1374 GenBank   NZ_CP018981
Plasmid name   pEC867_KPC Incompatibility group   ColRNAI
Plasmid size   14029 bp Coordinate of oriT [Strand]   7645..7823 [-]
Host baterium   Escherichia coli strain Ecol_867

Cargo genes

Drug resistance gene   blaKPC-3
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -