Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 100913 |
| Name | oriT_pEC545_KPC  |
| Organism | Escherichia coli strain Ecol_545 |
| Sequence Completeness | core |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP018974 (17430..17535 [+], 106 nt) |
| oriT length | 106 nt |
| IRs (inverted repeats) | 18..35, 41..58 (GTTTTTGGAGTACCGCCG..CGGCGGCCGTCCAAAAAC) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | predicted by the oriTfinder |
oriT sequence
Download Length: 106 nt
>oriT_pEC545_KPC
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 984 | GenBank | WP_078207386 |
| Name | PutativerelaxaseofpEC545_KPC |
UniProt ID | _ |
| Length | 659 a.a. | PDB ID | |
| Note | putative relaxase | ||
Relaxase protein sequence
Download Length: 659 a.a. Molecular weight: 75218.97 Da Isoelectric Point: 9.9948
>WP_078207386.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacteriaceae]
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLIRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSENDDKFNPK
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLIRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSENDDKFNPK
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 20331..47529
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| BE949_RS00680 (BE949_00710) | 15415..15726 | + | 312 | WP_004187333 | hypothetical protein | - |
| BE949_RS00685 (BE949_00715) | 15859..16395 | + | 537 | WP_004187332 | hypothetical protein | - |
| BE949_RS00690 (BE949_00720) | 16896..17261 | - | 366 | WP_039592107 | hypothetical protein | - |
| BE949_RS28045 | 17327..17854 | + | 528 | WP_241184337 | plasmid mobilization protein MobA | - |
| BE949_RS00700 (BE949_00730) | 17841..19820 | + | 1980 | WP_078207386 | TraI/MobA(P) family conjugative relaxase | - |
| BE949_RS00705 (BE949_00735) | 19834..20334 | + | 501 | WP_004187320 | DotD/TraH family lipoprotein | - |
| BE949_RS00710 (BE949_00740) | 20331..21110 | + | 780 | WP_004187315 | type IV secretory system conjugative DNA transfer family protein | traI |
| BE949_RS00715 (BE949_00745) | 21121..22284 | + | 1164 | WP_077138898 | plasmid transfer ATPase TraJ | virB11 |
| BE949_RS00720 (BE949_00750) | 22274..22534 | + | 261 | WP_004187310 | IcmT/TraK family protein | traK |
| BE949_RS28050 | 22559..25816 | + | 3258 | WP_223173498 | LPD7 domain-containing protein | - |
| BE949_RS00730 (BE949_00760) | 25782..26294 | + | 513 | WP_011091071 | hypothetical protein | traL |
| BE949_RS00735 (BE949_00765) | 26295..26507 | - | 213 | WP_305953721 | Hha/YmoA family nucleoid-associated regulatory protein | - |
| BE949_RS00740 (BE949_00770) | 26479..26889 | - | 411 | WP_004187465 | H-NS family nucleoid-associated regulatory protein | - |
| BE949_RS00745 (BE949_00775) | 26957..27670 | + | 714 | WP_004187467 | DotI/IcmL family type IV secretion protein | traM |
| BE949_RS00750 (BE949_00780) | 27679..28830 | + | 1152 | WP_004187471 | DotH/IcmK family type IV secretion protein | traN |
| BE949_RS00755 (BE949_00785) | 28842..30191 | + | 1350 | WP_004187474 | conjugal transfer protein TraO | traO |
| BE949_RS00760 (BE949_00790) | 30203..30907 | + | 705 | WP_015060002 | conjugal transfer protein TraP | traP |
| BE949_RS00765 (BE949_00795) | 30931..31461 | + | 531 | WP_004187478 | conjugal transfer protein TraQ | traQ |
| BE949_RS00770 (BE949_00800) | 31478..31867 | + | 390 | WP_004187479 | DUF6750 family protein | traR |
| BE949_RS00775 (BE949_00805) | 31913..32407 | + | 495 | WP_004187480 | hypothetical protein | - |
| BE949_RS00780 (BE949_00810) | 32404..35454 | + | 3051 | WP_078207387 | conjugal transfer protein | traU |
| BE949_RS00785 (BE949_00815) | 35451..36659 | + | 1209 | WP_011091082 | conjugal transfer protein TraW | traW |
| BE949_RS00790 (BE949_00820) | 36656..37306 | + | 651 | WP_004187488 | hypothetical protein | - |
| BE949_RS00795 (BE949_00825) | 37299..39479 | + | 2181 | WP_004187492 | DotA/TraY family protein | traY |
| BE949_RS00800 (BE949_00830) | 39482..40135 | + | 654 | WP_004206935 | hypothetical protein | - |
| BE949_RS00805 (BE949_00835) | 40209..40439 | + | 231 | WP_004187496 | IncL/M type plasmid replication protein RepC | - |
| BE949_RS00810 (BE949_00840) | 40736..41791 | + | 1056 | WP_249534829 | plasmid replication initiator RepA | - |
| BE949_RS28295 | 43015..43110 | + | 96 | WP_004206884 | DinQ-like type I toxin DqlB | - |
| BE949_RS00815 (BE949_00845) | 43161..45248 | - | 2088 | WP_004187443 | hypothetical protein | - |
| BE949_RS00820 (BE949_00850) | 45261..46211 | - | 951 | WP_078207389 | DsbC family protein | trbB |
| BE949_RS00825 (BE949_00855) | 46222..47529 | - | 1308 | WP_015062796 | hypothetical protein | trbA |
| BE949_RS00830 (BE949_00860) | 47529..47924 | - | 396 | WP_004187436 | lytic transglycosylase domain-containing protein | - |
| BE949_RS00835 (BE949_00865) | 48029..48379 | - | 351 | WP_004187434 | DUF1496 domain-containing protein | - |
| BE949_RS00840 (BE949_00870) | 48492..49145 | + | 654 | WP_039592068 | type II CAAX endopeptidase family protein | - |
| BE949_RS00845 (BE949_00875) | 49299..49538 | + | 240 | Protein_62 | S24 family peptidase | - |
| BE949_RS00850 (BE949_00880) | 49603..50307 | + | 705 | WP_001067855 | IS6-like element IS26 family transposase | - |
| BE949_RS00855 (BE949_00885) | 50451..51005 | + | 555 | WP_063840321 | fluoroquinolone-acetylating aminoglycoside 6'-N-acetyltransferase AAC(6')-Ib-cr5 | - |
| BE949_RS00860 (BE949_00890) | 51136..51966 | + | 831 | WP_001334766 | oxacillin-hydrolyzing class D beta-lactamase OXA-1 | - |
Host bacterium
| ID | 1372 | GenBank | NZ_CP018974 |
| Plasmid name | pEC545_KPC | Incompatibility group | IncL/M |
| Plasmid size | 70876 bp | Coordinate of oriT [Strand] | 17430..17535 [+] |
| Host baterium | Escherichia coli strain Ecol_545 |
Cargo genes
| Drug resistance gene | aac(6')-Ib-cr, blaOXA-1, catB3, blaKPC-2 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |