Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 100911 |
| Name | oriT_pEC542_KPC  |
| Organism | Escherichia coli strain Ecol_542 |
| Sequence Completeness | intact |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP018968 (30126..30434 [-], 309 nt) |
| oriT length | 309 nt |
| IRs (inverted repeats) | IR1:79..85, 89..95 (CAAGGGGC..GCCCCTTG) IR2: 153..160, 164..171 (CACGTGGC..GCCATGTG) |
| Location of nic site | 190..191 |
| Conserved sequence flanking the nic site |
CATCCTG|T |
| Note | predicted by the oriTfinder |
oriT sequence
Download Length: 309 nt
>oriT_pEC542_KPC
CGTGTCTCCTTCTCGCCGGGGCGGGGGGCGGGGTGTGTCTTGGACAGTAAGAAGCGAGAGCGCCGCACTGTCCGGGGTCAAGGGGATACCCCTTGCAGGATAAGCCAGACCGTGAGAAGCGAAGCGCCGAATGGGCAGGCGTCCGTTGGAACCACGTGGCGAAGCCATGTGTGTTACAACGGTCATCCTGTATTGCTCATCCGCTCTACTATCGTATCAGCAGTTTAGGTGCACGGGACGCACTTTGCAACAAGCACAAATGGCGCTATGCTGTCAACTCCGCAACAAGTAAGCAAAGGAGTTAGCACG
CGTGTCTCCTTCTCGCCGGGGCGGGGGGCGGGGTGTGTCTTGGACAGTAAGAAGCGAGAGCGCCGCACTGTCCGGGGTCAAGGGGATACCCCTTGCAGGATAAGCCAGACCGTGAGAAGCGAAGCGCCGAATGGGCAGGCGTCCGTTGGAACCACGTGGCGAAGCCATGTGTGTTACAACGGTCATCCTGTATTGCTCATCCGCTCTACTATCGTATCAGCAGTTTAGGTGCACGGGACGCACTTTGCAACAAGCACAAATGGCGCTATGCTGTCAACTCCGCAACAAGTAAGCAAAGGAGTTAGCACG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 982 | GenBank | WP_033996285 |
| Name | Mob_pEC542_KPC |
UniProt ID | A0A1J8JDJ9 |
| Length | 890 a.a. | PDB ID | |
| Note | putative relaxase | ||
Relaxase protein sequence
Download Length: 890 a.a. Molecular weight: 101454.99 Da Isoelectric Point: 9.8485
>WP_033996285.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Pseudomonadota]
MIVKKVKNPQKAASKAVRVSRLTDYIREPERENSQEKCIHAGARGFITDEPQSQTAEMIALSQEAVRSKD
TINHYVLSWREGEQPSPEQVEEAVSIFMDELGVKDHQAIYGLHADTDNLHLHLAINRVHPETLKVVKINN
GFDIEAAHKAIARIENAQGWQREQNGRYQVLENGELGREHIDKDKPRQPAQPKRDMENRTGEKSAERIAI
EDGAPIIKKAQTWEQLHRELAAKGMRYEKTGSGATLFVGDVGVKASSADRDASLSKLQKRLGAYQPPPQR
QQVAQREPEPIKPDVPGWKDYITGRKAHYAEKNAAKLALDKRQEQERKQIAEQQKARRDELMRGNWKGKG
EVLNAMRSVIAAEQAAEKAALKEKHQKQREQHRQQFRPYPDLEQWQRMQKSPELAEQWRHRASEPQRIEG
ASGEPPTPRDIRAYQPEIVGQQVHYSRKEEAGAGGGVSFVDKGKSIDIHDWRNRDSTLAALQLSAQKWGS
FTVTGNDEYKAMCAKLAAEHGFKITNPELQERIQQERQRIQQERAQAMKSEQLKQFELYAEAVGAERYRV
TSIKMQADGRKQTFILDKKDGITRGFTPQEIEQRTPEMLRLQRRGENLYYTPLSDKKHHILIDDMNREKL
ERLIRDGYRPAVVLESSPGNYQAIITVPKLGTAHDKDVGNRLSDALNREYGDPKLSGAIHPHRAPGYENR
KPKHQREDGSYPEVRLLKAERRECVKALALSSQIDAEYQRQAALKAQQPERSKAKPALELAAASGSAIDA
YQRHYRDVIKRQRGGEVDLSRVDSMIAVRMRVTGHDQAAIEGAIRQCAPATRQKDEGRDWNDYAQRTARY
AYSAAGDRQAAELGKYRQQWEKLEGREPVRQQEQAKAQKIERDNSPGMSR
MIVKKVKNPQKAASKAVRVSRLTDYIREPERENSQEKCIHAGARGFITDEPQSQTAEMIALSQEAVRSKD
TINHYVLSWREGEQPSPEQVEEAVSIFMDELGVKDHQAIYGLHADTDNLHLHLAINRVHPETLKVVKINN
GFDIEAAHKAIARIENAQGWQREQNGRYQVLENGELGREHIDKDKPRQPAQPKRDMENRTGEKSAERIAI
EDGAPIIKKAQTWEQLHRELAAKGMRYEKTGSGATLFVGDVGVKASSADRDASLSKLQKRLGAYQPPPQR
QQVAQREPEPIKPDVPGWKDYITGRKAHYAEKNAAKLALDKRQEQERKQIAEQQKARRDELMRGNWKGKG
EVLNAMRSVIAAEQAAEKAALKEKHQKQREQHRQQFRPYPDLEQWQRMQKSPELAEQWRHRASEPQRIEG
ASGEPPTPRDIRAYQPEIVGQQVHYSRKEEAGAGGGVSFVDKGKSIDIHDWRNRDSTLAALQLSAQKWGS
FTVTGNDEYKAMCAKLAAEHGFKITNPELQERIQQERQRIQQERAQAMKSEQLKQFELYAEAVGAERYRV
TSIKMQADGRKQTFILDKKDGITRGFTPQEIEQRTPEMLRLQRRGENLYYTPLSDKKHHILIDDMNREKL
ERLIRDGYRPAVVLESSPGNYQAIITVPKLGTAHDKDVGNRLSDALNREYGDPKLSGAIHPHRAPGYENR
KPKHQREDGSYPEVRLLKAERRECVKALALSSQIDAEYQRQAALKAQQPERSKAKPALELAAASGSAIDA
YQRHYRDVIKRQRGGEVDLSRVDSMIAVRMRVTGHDQAAIEGAIRQCAPATRQKDEGRDWNDYAQRTARY
AYSAAGDRQAAELGKYRQQWEKLEGREPVRQQEQAKAQKIERDNSPGMSR
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
Host bacterium
| ID | 1370 | GenBank | NZ_CP018968 |
| Plasmid name | pEC542_KPC | Incompatibility group | IncP6 |
| Plasmid size | 44320 bp | Coordinate of oriT [Strand] | 30126..30434 [-] |
| Host baterium | Escherichia coli strain Ecol_542 |
Cargo genes
| Drug resistance gene | blaKPC-2 |
| Virulence gene | - |
| Metal resistance gene | mercury resistance |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |