Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100910
Name   oriT_pEC422_KPC in_silico
Organism   Escherichia coli strain Ecol_422
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NZ_CP018959 (47300..47926 [+], 627 nt)
oriT length   627 nt
IRs (inverted repeats)      IR1: 198..207, 211..220  (ATTGGGTGTT..AATACCTAAT)
 IR2:319..324, 330..335  (AAAAAA..TTTTTT)
  IR3: 393..402, 405..414  (GTATTCATGC..GCATGAATAC)
Location of nic site      301..302
Conserved sequence flanking the
  nic site    		  GGTGT|ATAGC
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 627 nt

>oriT_pEC422_KPC
AGCGCCGCAGATAATCTGACCGATTACCTCCTGAAACCAGGTCTATATAGGCCAAAAGTTCATCTGATACTTTTGCGGTTATTATTGGCATTCAGTCCTCACATTGTGCATTTTTTAAACAAAAAATTGGGATCTAACAAGCTGAAATCTTAGTATTACCAAAGTAATAAAGCAAACTCATTATAAAACAATGAGTTATTGGGTGTTTTTAATACCTAATTATTACCGAATATTGTTGCTATTTATTTTTTTATCTTTTAAATCAGTATGATAGCGTGATTTATCGCGCTGCGTTAGGTGTATAGCAGGTTAAGGGATAAAAAATCATCTTTTTTGGTAGGGGCGATCTACGTAGGTTAAGGACTAACTGGCTAAAAAGCGTTCAATATTCCGTATTCATGCTTGCATGAATACCAGTACAACAAAAGTACATCAAAATTACATCAAAATTACATCACTTGAAGGTTGACAGTACAACAAAATTACATCATTCTTTGGTCATGAGGTAGCCAGTACAACAAAAGTACATCAAAAGTACATCAAAATTACATCAAAATTACATCATTCTAAATGAGGGTACTATGAAGCCCAAAAGTATCAGGGCGGCACTTCAGTTGATGTTGCCGG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   981 GenBank   WP_001749977
Name   Relaxase_pEC422_KPC insolico UniProt ID   _
Length   1080 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 1080 a.a.        Molecular weight: 120419.59 Da        Isoelectric Point: 6.5483

>WP_001749977.1 MULTISPECIES: MobF family relaxase [Gammaproteobacteria]
MLDITTITRQNVTSVVGYYSDAKDDYYSKDSSFTSWQGTGAEALGLSGDVESARFKELLVGEIDTFTHMQ
RHVGDAKKERLGYDLTFSAPKGVSMQALIHGDKTIIEAHEKAVAAAVREAEKLAQARTTRQGKSVTQNTN
NLVVATFRHETSRALDPDLHTHAFVMNMTQREDGQWRALKNDELMRNKMHLGDVYKQELALELTKAGYEL
RYNSKNNTFDMAHFSDEQIRAFSRRSEQIEKGLAAMGLTRETADAQTKSRVSMATREKKTEHSREEIHQE
WASRAKTLGIDFDNREWQGHGKPLEADIARNMAPDFTSPEVKADRAIQFAVKSLSERDASFERQKLIQIA
NKQVLGHATIADVEKAYLKAVQKGAIIEGEARYQSTLKVGASVMAETLTRKEWIDSLTNSGMRADKARFA
VDDGIKNGRLKKTSHRVTTVEGIRLERSILTIESRGRGQMPRQLTAEIAGQLLAGKTLKKEQMRAVTEIV
TSKDRFVAAHGYAGTGKSYMTMAAKELLESQGLKVTALAPYGTQKKALEDDGLPARTVAAFLKAKDKKLD
EKSVVFIDEAGVIPARQMKQLMEVIEKHNARAVFLGDTSQTKAVEAGKPFEQLIKAGMQTSYMKDIQRQK
NEVLLEAVKYAAEGNAARALKNITGVNELKEEAPRLSQLADRYLSLSSEQQDATLIISGTNASRKTLNDY
IRGNLGLAGTGETFTLLDRVDSTQAERRDSRYFSKGQIIIPEQDYKNGMKRGESYQVLDTGPGNKLTVES
SSGEQIAFSPRTHTKLSVYQAVSAELAPGDKVMVTRNDKTLDVANGDRFTVKTVEGEKLTLEDKKGRTVE
LDKKQASYLSYAYATTVHKSQGLTCDRVLFNIDTKSLTTSKDVFYVGISRARHEVEIFTDDKKSLASSVS
RDSPKTTAAEIDRFFGLEARFKDIGRDTSLETRSAEKGLPEATGESMAFNQKPDEHNMTTGTDYQPVSNA
EDAFHLKQNPMDDSVGLRRHEAQQNDAELAHDYAAADDQQWSAQEYADYEHYAEASDYDFDSSIYDDYAM
PQTSQAEQSHTGKEHTHEHEHEHEEGGHEI

  Protein domains


Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C_2)

(851-895)

TrwC relaxase (TrwC)

(16-287)

AAA domain (AAA_30)

(477-653)


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 24080..34405

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BE933_RS00535 (BE933_00540) 19925..21328 - 1404 WP_072094655 DNA cytosine methyltransferase -
BE933_RS00540 (BE933_00545) 21457..21729 + 273 Protein_26 IS1 family transposase -
BE933_RS00545 (BE933_00550) 21740..21946 - 207 WP_001749967 hypothetical protein -
BE933_RS00550 (BE933_00555) 21951..22592 - 642 WP_001749966 restriction endonuclease -
BE933_RS00555 (BE933_00560) 22648..22959 - 312 WP_001452736 hypothetical protein -
BE933_RS00560 (BE933_00565) 22995..23309 - 315 WP_001749965 TrbM/KikA/MpfK family conjugal transfer protein -
BE933_RS00565 (BE933_00570) 23306..23650 - 345 WP_001749964 hypothetical protein -
BE933_RS00570 (BE933_00575) 23666..24016 - 351 WP_024129965 H-NS family nucleoid-associated regulatory protein -
BE933_RS00575 (BE933_00580) 24080..24814 + 735 WP_001749963 lytic transglycosylase domain-containing protein virB1
BE933_RS00580 (BE933_00585) 24823..25104 + 282 WP_000440698 transcriptional repressor KorA -
BE933_RS00585 (BE933_00590) 25114..25407 + 294 WP_001749962 hypothetical protein virB2
BE933_RS00590 (BE933_00595) 25457..25774 + 318 WP_000496058 VirB3 family type IV secretion system protein virB3
BE933_RS00595 (BE933_00600) 25774..28374 + 2601 WP_001749961 VirB4 family type IV secretion/conjugal transfer ATPase virb4
BE933_RS00600 (BE933_00605) 28392..29105 + 714 WP_001749960 type IV secretion system protein virB5
BE933_RS00605 (BE933_00610) 29113..29340 + 228 WP_001749959 IncN-type entry exclusion lipoprotein EexN -
BE933_RS00610 (BE933_00615) 29356..30396 + 1041 WP_001749958 type IV secretion system protein virB6
BE933_RS00615 (BE933_00620) 30479..30625 + 147 WP_001257173 conjugal transfer protein TraN -
BE933_RS00620 (BE933_00625) 30615..31313 + 699 WP_000646594 type IV secretion system protein virB8
BE933_RS00625 (BE933_00630) 31324..32208 + 885 WP_000735066 TrbG/VirB9 family P-type conjugative transfer protein virB9
BE933_RS00630 (BE933_00635) 32208..33368 + 1161 WP_000101710 type IV secretion system protein VirB10 virB10
BE933_RS00635 (BE933_00640) 33410..34405 + 996 WP_000128596 ATPase, T2SS/T4P/T4SS family virB11
BE933_RS00640 (BE933_00645) 34405..34938 + 534 WP_000792636 phospholipase D family protein -
BE933_RS27185 35394..35606 - 213 Protein_47 FipA -
BE933_RS00650 (BE933_00655) 35980..36291 + 312 Protein_48 transposase -
BE933_RS00655 (BE933_00660) 36541..37422 - 882 WP_004199234 carbapenem-hydrolyzing class A beta-lactamase KPC-2 -
BE933_RS00660 (BE933_00665) 37644..38216 - 573 Protein_50 class A beta-lactamase -
BE933_RS00665 (BE933_00670) 38386..38943 - 558 WP_001217881 recombinase family protein -

Host bacterium

ID   1369 GenBank   NZ_CP018959
Plasmid name   pEC422_KPC Incompatibility group   IncN
Plasmid size   51885 bp Coordinate of oriT [Strand]   47300..47926 [+]
Host baterium   Escherichia coli strain Ecol_422

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -