Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100908
Name   oriT_pEC316_KPC in_silico
Organism   Escherichia coli strain Ecol_316
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NZ_CP018956 (90613..91163 [+], 551 nt)
oriT length   551 nt
IRs (inverted repeats)      IR1: 62..70, 71..79  (AACCCTTTC..GACAGGGTT)
  IR2: 143..150, 154..161  (TGGCCTGC..GGAGGCCA)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 551 nt

>oriT_pEC316_KPC
TGTAAAGAGGCTGTGAAAGAATAAGAGCATCAAGATTCCAGATAGATAGAGGGAAATTTGACAAATTCCAAAGATGGGTTAGCCTAGTGACAGAACTAGATTCCAGTATTGGAATAATCAGCTTTAAATTCCAGATAGATAGTTATGTGGATAGGAATTGGATAGGAATTGGGAGGGTATTGAGG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   979 GenBank   WP_001337757
Name   TraI_pEC316_KPC insolico UniProt ID   G0XAC3
Length   992 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 992 a.a.        Molecular weight: 109581.85 Da        Isoelectric Point: 4.8956

>WP_001337757.1 MULTISPECIES: MobH family relaxase [Gammaproteobacteria]
MLKALNKLFGGRSGVIETAPSARVLPLKDVEDEEIPRYPPFAKGLPVAPLDKILATQAELIEKVRNSLGF
TVDDFNRLVLPVIQRYAAFVHLLPASESHHHRGAGGLFRHGLEVAFWAAQASESVIFSIEGTPRERRDNE
PRWRLASCFSGLLHDVGKPLSDVSITDKDGSITWNPYSESLHDWAHRHEIDRYFIRWRDKRHKRHEQFSL
LAVDRIIPAETREFLSKSGPSIMEAMLEAISGTSVNQPVTKLMLRADQESVSRDLRQSRLDVDEFSYGVP
VERYVFDAIRRLVKTGKWKVNEPGAKVWHLNQGVFIAWKQLGDLYDLISHDKIPGIPRDPDTLADILIER
GFAVPNTVQEKGERAYYRYWEVLPEMLQEAAGSVKILMLRLESNDLVFTTEPPAAVAAEVVGDVEDAEIE
FVDPEEADDGDDQEEGEAALNDDMLAAEQEAEKALAGLGFGDAMEMLKSTSDAVEEKPEQKDAGPTESSK
PDAGKKGKPQSKPGKAKPKSDTEKQPHKPEAKEDLSPQDIAKNAPPLANDNPLQALKDVGGGLGDIDFPF
DAFNASAETTSTDATNSEIPDVAMPGKQEEQPKQDFVPQEQNSLQGDDFPMFGGSDEPPSWAIEPLPMLT
DAPEQPTHTPEMPHTDNVNQHEKDAKTLLVEMLSGYGEASALLEQAIMPVLEGKTTLGEVLCLMKGQAVI
LYPDGARSLGAPSEVLSKLSHANAIVPDPIMPGRKVRDFSGVKAIVLAEQLSDAVVAAIKDAEASMGGYQ
DAFELVSPPGLDASKNKSAPKQQSRKKAQQQKPEVNAGKASPEQKAKGKDSQPQPKEKKVDVTSPVEEQQ
RKPVQEKQNVARLPKREAQPVAPEPKVEREKELGHVEVREREDPEVREFEPPKAKTNPKDINAEDFLPSG
VTPQKALQMLKDMIQKRSGRWLVTPVLEEDGCLVTSDKAFDMIAGENIGISKHILCGMLSRAQRRPLLKK
RQGKLYLEVNET

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(51-357)


  Protein structure


Source ID Structure
AlphaFold DB G0XAC3


T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 88747..116354

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BE963_RS01900 (BE963_01945) 84772..85005 - 234 WP_001191892 hypothetical protein -
BE963_RS01905 (BE963_01950) 84987..85604 - 618 WP_001249396 hypothetical protein -
BE963_RS01910 (BE963_01955) 85772..88750 + 2979 WP_001337757 MobH family relaxase -
BE963_RS01915 (BE963_01960) 88747..90612 + 1866 WP_000178856 conjugative transfer system coupling protein TraD virb4
BE963_RS01920 (BE963_01965) 90662..91207 + 546 WP_000228720 hypothetical protein -
BE963_RS01925 (BE963_01970) 91164..91793 + 630 WP_000743450 DUF4400 domain-containing protein tfc7
BE963_RS01930 (BE963_01975) 91803..92249 + 447 WP_000122506 hypothetical protein -
BE963_RS01935 (BE963_01980) 92259..92636 + 378 WP_000869261 hypothetical protein -
BE963_RS01940 (BE963_01985) 92636..93298 + 663 WP_001231463 hypothetical protein -
BE963_RS29360 93479..93622 + 144 WP_001275801 hypothetical protein -
BE963_RS01945 (BE963_01990) 93634..93999 + 366 WP_001052531 hypothetical protein -
BE963_RS01950 (BE963_01995) 94144..94425 + 282 WP_000805625 type IV conjugative transfer system protein TraL traL
BE963_RS01955 (BE963_02000) 94422..95048 + 627 WP_001049716 TraE/TraK family type IV conjugative transfer system protein traE
BE963_RS01960 (BE963_02005) 95032..95949 + 918 WP_000794249 type-F conjugative transfer system secretin TraK traK
BE963_RS01965 (BE963_02010) 95949..97262 + 1314 WP_024131605 TraB/VirB10 family protein traB
BE963_RS01970 (BE963_02015) 97259..97837 + 579 WP_000793435 type IV conjugative transfer system lipoprotein TraV traV
BE963_RS01975 (BE963_02020) 97841..98233 + 393 WP_000479535 TraA family conjugative transfer protein -
BE963_RS01980 (BE963_02025) 98434..103965 + 5532 WP_000606833 Ig-like domain-containing protein -
BE963_RS01985 (BE963_02030) 104114..104473 + 360 WP_249532917 disulfide isomerase DsbC N-terminal domain-containing protein -
BE963_RS01990 (BE963_02035) 104510..106009 + 1500 WP_000174402 IS21-like element ISEc10 family transposase -
BE963_RS01995 (BE963_02040) 106006..106761 + 756 WP_000065240 IS21-like element ISEc10 family helper ATPase IstB -
BE963_RS02000 (BE963_02045) 106830..107237 + 408 WP_249532918 thioredoxin fold domain-containing protein -
BE963_RS02005 (BE963_02050) 107234..109681 + 2448 WP_000637386 type IV secretion system protein TraC virb4
BE963_RS02010 (BE963_02055) 109696..110013 + 318 WP_000351984 hypothetical protein -
BE963_RS02015 (BE963_02060) 110010..110540 + 531 WP_001010738 S26 family signal peptidase -
BE963_RS02020 (BE963_02065) 110503..111768 + 1266 WP_000621288 TrbC family F-type conjugative pilus assembly protein traW
BE963_RS02025 (BE963_02070) 111765..112436 + 672 WP_001337754 EAL domain-containing protein -
BE963_RS02030 (BE963_02075) 112436..113440 + 1005 WP_043940352 TraU family protein traU
BE963_RS02035 (BE963_02080) 113556..116354 + 2799 WP_001256487 conjugal transfer mating pair stabilization protein TraN traN
BE963_RS02040 (BE963_02085) 116393..117253 - 861 WP_000709517 hypothetical protein -
BE963_RS02045 (BE963_02090) 117376..118017 - 642 WP_000796664 hypothetical protein -
BE963_RS02050 (BE963_02095) 118311..118631 + 321 WP_000547566 hypothetical protein -
BE963_RS02055 (BE963_02100) 118935..119120 + 186 WP_001186917 hypothetical protein -
BE963_RS02060 (BE963_02105) 119340..120308 + 969 WP_000085162 AAA family ATPase -
BE963_RS02065 (BE963_02110) 120319..121227 + 909 WP_000739139 hypothetical protein -

Host bacterium

ID   1367 GenBank   NZ_CP018956
Plasmid name   pEC316_KPC Incompatibility group   IncA/C2
Plasmid size   216226 bp Coordinate of oriT [Strand]   90613..91163 [+]
Host baterium   Escherichia coli strain Ecol_316

Cargo genes

Drug resistance gene   blaTEM-1D, ant(2'')-Ia, aac(6')-33, qacE, sul1, blaKPC-2, sul2, floR
Virulence gene   -
Metal resistance gene   merR, merT, merP, merC, merA, merD, merE
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -