Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100905
Name   oriT_pEC224_KPC in_silico
Organism   Escherichia coli strain Ecol_224
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NZ_CP018945 (21572..22198 [-], 627 nt)
oriT length   627 nt
IRs (inverted repeats)      IR1: 198..207, 211..220  (ATTGGGTGTT..AATACCTAAT)
 IR2:319..324, 330..335  (AAAAAA..TTTTTT)
  IR3: 393..402, 405..414  (GTATTCATGC..GCATGAATAC)
Location of nic site      301..302
Conserved sequence flanking the
  nic site    		  GGTGT|ATAGC
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 627 nt

>oriT_pEC224_KPC
AGCGCCGCAGATAATCTGACCGATTACCTCCTGAAACCAGGTCTATATAGGCCAAAAGTTCATCTGATACTTTTGCGGTTATTATTGGCATTCAGTCCTCACATTGTGCATTTTTTAAACAAAAAATTGGGATCTAACAAGCTGAAATCTTAGTATTACCAAAGTAATAAAGCAAACTCATTATAAAACAATGAGTTATTGGGTGTTTTTAATACCTAATTATTACCGAATATTGTTGCTATTTATTTTTTTATCTTTTAAATCAGTATGATAGCGTGATTTATCGCGCTGCGTTAGGTGTATAGCAGGTTAAGGGATAAAAAATCATCTTTTTTGGTAGGGGCGATCTACGTAGGTTAAGGACTAACTGGCTAAAAAGCGTTCAATATTCCGTATTCATGCTTGCATGAATACCAGTACAACAAAAGTACATCAAAATTACATCAAAATTACATCACTTGAAGGTTGACAGTACAACAAAATTACATCATTCTTTGGTCATGAGGTAGCCAGTACAACAAAAGTACATCAAAAGTACATCAAAATTACATCAAAATTACATCATTCTAAATGAGGGTACTATGAAGCCCAAAAGTATCAGGGCGGCACTTCAGTTGATGTTGCCGG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   976 GenBank   WP_001749977
Name   Relaxase_pEC224_KPC insolico UniProt ID   F6IB82
Length   1080 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 1080 a.a.        Molecular weight: 120419.59 Da        Isoelectric Point: 6.5483

>WP_001749977.1 MULTISPECIES: MobF family relaxase [Gammaproteobacteria]
MLDITTITRQNVTSVVGYYSDAKDDYYSKDSSFTSWQGTGAEALGLSGDVESARFKELLVGEIDTFTHMQ
RHVGDAKKERLGYDLTFSAPKGVSMQALIHGDKTIIEAHEKAVAAAVREAEKLAQARTTRQGKSVTQNTN
NLVVATFRHETSRALDPDLHTHAFVMNMTQREDGQWRALKNDELMRNKMHLGDVYKQELALELTKAGYEL
RYNSKNNTFDMAHFSDEQIRAFSRRSEQIEKGLAAMGLTRETADAQTKSRVSMATREKKTEHSREEIHQE
WASRAKTLGIDFDNREWQGHGKPLEADIARNMAPDFTSPEVKADRAIQFAVKSLSERDASFERQKLIQIA
NKQVLGHATIADVEKAYLKAVQKGAIIEGEARYQSTLKVGASVMAETLTRKEWIDSLTNSGMRADKARFA
VDDGIKNGRLKKTSHRVTTVEGIRLERSILTIESRGRGQMPRQLTAEIAGQLLAGKTLKKEQMRAVTEIV
TSKDRFVAAHGYAGTGKSYMTMAAKELLESQGLKVTALAPYGTQKKALEDDGLPARTVAAFLKAKDKKLD
EKSVVFIDEAGVIPARQMKQLMEVIEKHNARAVFLGDTSQTKAVEAGKPFEQLIKAGMQTSYMKDIQRQK
NEVLLEAVKYAAEGNAARALKNITGVNELKEEAPRLSQLADRYLSLSSEQQDATLIISGTNASRKTLNDY
IRGNLGLAGTGETFTLLDRVDSTQAERRDSRYFSKGQIIIPEQDYKNGMKRGESYQVLDTGPGNKLTVES
SSGEQIAFSPRTHTKLSVYQAVSAELAPGDKVMVTRNDKTLDVANGDRFTVKTVEGEKLTLEDKKGRTVE
LDKKQASYLSYAYATTVHKSQGLTCDRVLFNIDTKSLTTSKDVFYVGISRARHEVEIFTDDKKSLASSVS
RDSPKTTAAEIDRFFGLEARFKDIGRDTSLETRSAEKGLPEATGESMAFNQKPDEHNMTTGTDYQPVSNA
EDAFHLKQNPMDDSVGLRRHEAQQNDAELAHDYAAADDQQWSAQEYADYEHYAEASDYDFDSSIYDDYAM
PQTSQAEQSHTGKEHTHEHEHEHEEGGHEI

  Protein domains


Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C_2)

(851-895)

TrwC relaxase (TrwC)

(16-287)

AAA domain (AAA_30)

(477-653)


  Protein structure


Source ID Structure
AlphaFold DB F6IB82


T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 37549..47874

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BE957_RS00480 (BE957_00490) 32971..33951 + 981 WP_013213985 IS481-like element ISKpn27 family transposase -
BE957_RS00485 (BE957_00495) 34233..35114 + 882 WP_004199234 carbapenem-hydrolyzing class A beta-lactamase KPC-2 -
BE957_RS00490 (BE957_00500) 35364..36104 - 741 Protein_38 IS1182-like element ISKpn6 family transposase -
BE957_RS28615 (BE957_00505) 36390..36842 + 453 WP_001749956 DUF6710 family protein -
BE957_RS00500 (BE957_00510) 37016..37549 - 534 WP_000792636 phospholipase D family protein -
BE957_RS00505 (BE957_00515) 37549..38544 - 996 WP_000128596 ATPase, T2SS/T4P/T4SS family virB11
BE957_RS00510 (BE957_00520) 38586..39746 - 1161 WP_000101710 type IV secretion system protein VirB10 virB10
BE957_RS00515 (BE957_00525) 39746..40630 - 885 WP_000735066 TrbG/VirB9 family P-type conjugative transfer protein virB9
BE957_RS00520 (BE957_00530) 40641..41339 - 699 WP_000646594 type IV secretion system protein virB8
BE957_RS00525 (BE957_00535) 41329..41475 - 147 WP_001257173 conjugal transfer protein TraN -
BE957_RS00530 (BE957_00540) 41558..42598 - 1041 WP_001749958 type IV secretion system protein virB6
BE957_RS00535 (BE957_00545) 42614..42841 - 228 WP_001749959 IncN-type entry exclusion lipoprotein EexN -
BE957_RS00540 (BE957_00550) 42849..43562 - 714 WP_001749960 type IV secretion system protein virB5
BE957_RS00545 (BE957_00555) 43580..46180 - 2601 WP_001749961 VirB4 family type IV secretion/conjugal transfer ATPase virb4
BE957_RS00550 (BE957_00560) 46180..46497 - 318 WP_000496058 VirB3 family type IV secretion system protein virB3
BE957_RS00555 (BE957_00565) 46547..46840 - 294 WP_001749962 hypothetical protein virB2
BE957_RS00560 (BE957_00570) 46850..47131 - 282 WP_000440698 transcriptional repressor KorA -
BE957_RS00565 (BE957_00575) 47140..47874 - 735 WP_001749963 lytic transglycosylase domain-containing protein virB1
BE957_RS00570 (BE957_00580) 47917..48288 + 372 WP_011867773 H-NS family nucleoid-associated regulatory protein -
BE957_RS00575 (BE957_00585) 48304..48648 + 345 WP_001749964 hypothetical protein -
BE957_RS00580 (BE957_00590) 48645..48959 + 315 WP_001749965 TrbM/KikA/MpfK family conjugal transfer protein -
BE957_RS00585 (BE957_00595) 48995..49306 + 312 WP_001452736 hypothetical protein -
BE957_RS00590 (BE957_00600) 49356..50003 + 648 WP_015344958 restriction endonuclease -
BE957_RS00595 (BE957_00605) 50008..50214 + 207 WP_001749967 hypothetical protein -
BE957_RS00600 (BE957_00610) 50225..50497 - 273 Protein_60 IS1 family transposase -
BE957_RS00605 (BE957_00615) 50596..52029 + 1434 WP_001288432 DNA cytosine methyltransferase -

Host bacterium

ID   1364 GenBank   NZ_CP018945
Plasmid name   pEC224_KPC Incompatibility group   IncN
Plasmid size   55436 bp Coordinate of oriT [Strand]   21572..22198 [-]
Host baterium   Escherichia coli strain Ecol_224

Cargo genes

Drug resistance gene   sul1, qacE, aadA16, dfrA27, ARR-3, aac(6')-Ib-cr, blaKPC-2
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -