Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100904
Name   oriT_pEC224_5 in_silico
Organism   Escherichia coli strain Ecol_224
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NZ_CP018943 (5449..5531 [+], 83 nt)
oriT length   83 nt
IRs (inverted repeats)      3..12, 16..25  (GTGTCGGGGC..GCCCTGACCC)
Location of nic site      56..57
Conserved sequence flanking the
  nic site    		  CTGG|CTTA
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 83 nt

>oriT_pEC224_5
GGGTGTCGGGGCGCAGCCCTGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACCATGCGGCATCAGTGCGGATT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   975 GenBank   WP_024623332
Name   PutativerelaxaseofpEC224_5 insolico UniProt ID   A0A1U9SBD2
Length   517 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 517 a.a.        Molecular weight: 57794.27 Da        Isoelectric Point: 6.0811

>WP_024623332.1 MULTISPECIES: relaxase/mobilization nuclease domain-containing protein [Enterobacteriaceae]
MIVKFHARGKGGGSGPVDYLLGRERNREGATVLQGNPEEVRELIDATPFAKKYTSGVLSFAEKELPPGGR
EKVMASFERVLMPGLEKNQYSILWVEHQDKGRLELNFVIPNMELQTGKRLQPYYDRADRPRIDAWQTLVN
HHYGLHDPNAPENRRTLTLPDNLPETKQALAEGVTRGIDALYHAGEIKGRQDVIQALTEAGLEVVRVTRS
SISIADPNGGKNIRLKGAFYEQSFADGRGVREKAERESRIYRENAEQRVQEARRICKRGCDIKRDENQRR
YSPVHSLDRGIAGKTPGRGERGDDAAQEGRVKAGREYGHDVTGDSISPVYREWRDALVSWREDTGEPGRN
QEAGRDIAETEREDMGRGVCAGREQEIPCPSVREISGGDSLSGERVGTSEGVTQSDRAGNTFAERLRAAA
TGLYAAAERMGERLRGIAEDVFAYATGQRDAERAGHAVESAGAALERADRTLEPVIQRELEIREERLIQE
REHVLSLERERQPEIQERTLDGPSLGW

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(57-237)


  Protein structure



No available structure.




Host bacterium

ID   1363 GenBank   NZ_CP018943
Plasmid name   pEC224_5 Incompatibility group   ColRNAI
Plasmid size   6647 bp Coordinate of oriT [Strand]   5449..5531 [+]
Host baterium   Escherichia coli strain Ecol_224

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -