Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 100903 |
| Name | oriT_unitig_1-pAR_0049  |
| Organism | Klebsiella pneumoniae strain AR_0049 |
| Sequence Completeness | intact |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP018817 (106944..107494 [-], 551 nt) |
| oriT length | 551 nt |
| IRs (inverted repeats) | IR1: 62..70, 71..79 (AACCCTTTC..GACAGGGTT) IR2: 143..150, 154..161 (TGGCCTGC..GGAGGCCA) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | predicted by the oriTfinder |
oriT sequence
Download Length: 551 nt
>oriT_unitig_1-pAR_0049
GAGCAGAGCTATGTGTGACAAGAAGTATAGAAATTACGAGGTAGCCATCATGGTCGATGTGAACCCTTTCGACAGGGTTATGAATGAATTGAAAAGTCGTGGCCGCAAGAACGCTCACATCCTGAGCATCCTCCAATTCGACTGGCCTGCATCGGAGGCCATCATCGAGAAGCTGAGCTGCTACATCACAGACGGGATTAAGGCTAATCAGGAGCCTGTGATTTACCCGATCATTGAAGAAGCTCTGCATCGCTACAGCCAGCTCGTGTTTCATGAGCAGAGAGAGAAATATGAAGACCCGGCCAGAATTGGGGCATTTCTGGAAACCCTGATCACCGAAACCTGCCGGGCGTTGGAAGTGCAAATTGTCGATAGTGGCGGTGATTCATGGTCTGTCGATTCAGGAGAGTCGTTCTCACTGTGGCTTTCTTCCCATCCAGGAGAACTATCCATTAACCCGCAGCCCCATGAGGATGAGACCTCTTTGCGTGGCTTGCTGTATGAGCTCATCACCTGTGAGAGCGTGAAAACTGTTTTAAGGAGAACCGACT
GAGCAGAGCTATGTGTGACAAGAAGTATAGAAATTACGAGGTAGCCATCATGGTCGATGTGAACCCTTTCGACAGGGTTATGAATGAATTGAAAAGTCGTGGCCGCAAGAACGCTCACATCCTGAGCATCCTCCAATTCGACTGGCCTGCATCGGAGGCCATCATCGAGAAGCTGAGCTGCTACATCACAGACGGGATTAAGGCTAATCAGGAGCCTGTGATTTACCCGATCATTGAAGAAGCTCTGCATCGCTACAGCCAGCTCGTGTTTCATGAGCAGAGAGAGAAATATGAAGACCCGGCCAGAATTGGGGCATTTCTGGAAACCCTGATCACCGAAACCTGCCGGGCGTTGGAAGTGCAAATTGTCGATAGTGGCGGTGATTCATGGTCTGTCGATTCAGGAGAGTCGTTCTCACTGTGGCTTTCTTCCCATCCAGGAGAACTATCCATTAACCCGCAGCCCCATGAGGATGAGACCTCTTTGCGTGGCTTGCTGTATGAGCTCATCACCTGTGAGAGCGTGAAAACTGTTTTAAGGAGAACCGACT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 974 | GenBank | WP_001326173 |
| Name | TraI_unitig_1-pAR_0049 |
UniProt ID | _ |
| Length | 990 a.a. | PDB ID | |
| Note | putative relaxase | ||
Relaxase protein sequence
Download Length: 990 a.a. Molecular weight: 109356.90 Da Isoelectric Point: 4.9395
>WP_001326173.1 MULTISPECIES: MobH family relaxase [Gammaproteobacteria]
MLKALNKLFGGRSGVIETAPSVRVLPLKDVEDEEIPRYPPFAKGLPVAPLDKILATQAELIEKVRNSLGF
TVDDFNRLVLPVIQRYAAFVHLLPASESHHHRGAGGLFRHGLEVAFWAAQASESVIFSIEGTPRERRDNE
PRWRLASCFSGLLHDVGKPLSDVSITDKDGSITWNPYSESLHDWAHRHEIDRYFIRWRDKRHKRHEQFSL
LAVDRIIPAETREFLSKSGPSIMEAMLEAISGTSVNQPVTKLMLRADQESVSRDLRQSRLDVDEFSYGVP
VERYVFDAIRRLVKTGKWKVNEPGAKVWHLNQGVFIAWKQLGDLYDLISHDKIPGIPRDPDTLADILIER
GFAVPNTVQEKGERAYYRYWEVLPEMLQEAAGSVKILMLRLESNDLVFTTEPPAAVAAEVVGDVEDAEIE
FVDPEEVDDDQEEDVSALNDDMLAAEQEAEKALAGLGFGDAMEMLKSTSDAVEEKPEQKDAGSTESSKPD
AGKKGKPQSKPGKAKPKSDTEKQPHKPEAKEDLSPQDIAKNAPPLANDNPLQALKDVGGGLGDIDFPFDA
FSASAETASTDATNSEIPDVAMPGKQEKQPKQDFVPQEQNSLQGDDFPMFGSSDEPPSWAIEPLPMLTDA
PEQTTPAPAMPPTDKPNLHEKDAKTLLVEMLAGYGEASALLEQAIMPVLEGKTTLGEVLCLMKGQAVILY
PDGARSLGAPSEVLSKLSHANAIVPDPIMPGRKVRDFSGVKAIVLAEQLSDAVVAAIKDAEASMGGYQDA
FELVSPPGLDASKNKSAPKQQSRKKAQQQKPEVNAGKPSPEQKAKGKDSQPQQKEKKVDVTSPVEEPQRQ
PVQEKQNVARLPKREVQPVAPEPKVEREKELGHVEVREREEPEVREFEPPKAKTNPKDINAEDFLPSGVT
PQKALQMLKDMIQKRSGRWLVTPVLEEDGCLVTSDKAFDMIAGENIGISKHILCGMLSRAQRRPLLKKRQ
GKLYLEVNET
MLKALNKLFGGRSGVIETAPSVRVLPLKDVEDEEIPRYPPFAKGLPVAPLDKILATQAELIEKVRNSLGF
TVDDFNRLVLPVIQRYAAFVHLLPASESHHHRGAGGLFRHGLEVAFWAAQASESVIFSIEGTPRERRDNE
PRWRLASCFSGLLHDVGKPLSDVSITDKDGSITWNPYSESLHDWAHRHEIDRYFIRWRDKRHKRHEQFSL
LAVDRIIPAETREFLSKSGPSIMEAMLEAISGTSVNQPVTKLMLRADQESVSRDLRQSRLDVDEFSYGVP
VERYVFDAIRRLVKTGKWKVNEPGAKVWHLNQGVFIAWKQLGDLYDLISHDKIPGIPRDPDTLADILIER
GFAVPNTVQEKGERAYYRYWEVLPEMLQEAAGSVKILMLRLESNDLVFTTEPPAAVAAEVVGDVEDAEIE
FVDPEEVDDDQEEDVSALNDDMLAAEQEAEKALAGLGFGDAMEMLKSTSDAVEEKPEQKDAGSTESSKPD
AGKKGKPQSKPGKAKPKSDTEKQPHKPEAKEDLSPQDIAKNAPPLANDNPLQALKDVGGGLGDIDFPFDA
FSASAETASTDATNSEIPDVAMPGKQEKQPKQDFVPQEQNSLQGDDFPMFGSSDEPPSWAIEPLPMLTDA
PEQTTPAPAMPPTDKPNLHEKDAKTLLVEMLAGYGEASALLEQAIMPVLEGKTTLGEVLCLMKGQAVILY
PDGARSLGAPSEVLSKLSHANAIVPDPIMPGRKVRDFSGVKAIVLAEQLSDAVVAAIKDAEASMGGYQDA
FELVSPPGLDASKNKSAPKQQSRKKAQQQKPEVNAGKPSPEQKAKGKDSQPQQKEKKVDVTSPVEEPQRQ
PVQEKQNVARLPKREVQPVAPEPKVEREKELGHVEVREREEPEVREFEPPKAKTNPKDINAEDFLPSGVT
PQKALQMLKDMIQKRSGRWLVTPVLEEDGCLVTSDKAFDMIAGENIGISKHILCGMLSRAQRRPLLKKRQ
GKLYLEVNET
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 100270..109360
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| AM428_RS33890 (AM428_29030) | 95295..95660 | - | 366 | Protein_104 | LuxR family transcriptional regulator | - |
| AM428_RS29235 (AM428_29035) | 95917..96234 | + | 318 | WP_000118520 | quaternary ammonium compound efflux SMR transporter SugE | - |
| AM428_RS29240 (AM428_29040) | 96231..96764 | - | 534 | WP_001221666 | lipocalin family protein | - |
| AM428_RS29245 (AM428_29045) | 96858..98003 | - | 1146 | WP_015058212 | class C beta-lactamase CMY-6 | - |
| AM428_RS29260 (AM428_29055) | 98327..99589 | - | 1263 | WP_000608644 | IS1380-like element ISEcp1 family transposase | - |
| AM428_RS29265 (AM428_29060) | 99874..100266 | - | 393 | WP_000479535 | TraA family conjugative transfer protein | - |
| AM428_RS29270 (AM428_29065) | 100270..100848 | - | 579 | WP_000793435 | type IV conjugative transfer system lipoprotein TraV | traV |
| AM428_RS29275 (AM428_29070) | 100845..102158 | - | 1314 | WP_024131605 | TraB/VirB10 family protein | traB |
| AM428_RS29280 (AM428_29075) | 102158..103075 | - | 918 | WP_000794249 | type-F conjugative transfer system secretin TraK | traK |
| AM428_RS29285 (AM428_29080) | 103059..103685 | - | 627 | WP_001049717 | TraE/TraK family type IV conjugative transfer system protein | traE |
| AM428_RS29290 (AM428_29085) | 103682..103963 | - | 282 | WP_000805625 | type IV conjugative transfer system protein TraL | traL |
| AM428_RS29295 (AM428_29090) | 104108..104473 | - | 366 | WP_001052530 | hypothetical protein | - |
| AM428_RS33485 | 104485..104628 | - | 144 | WP_001275801 | hypothetical protein | - |
| AM428_RS29300 (AM428_29095) | 104809..105471 | - | 663 | WP_001231464 | hypothetical protein | - |
| AM428_RS29305 (AM428_29100) | 105471..105848 | - | 378 | WP_000869297 | hypothetical protein | - |
| AM428_RS29310 (AM428_29105) | 105858..106304 | - | 447 | WP_000122507 | hypothetical protein | - |
| AM428_RS29315 (AM428_29110) | 106314..106943 | - | 630 | WP_000743449 | DUF4400 domain-containing protein | tfc7 |
| AM428_RS29320 (AM428_29115) | 106900..107445 | - | 546 | WP_000228720 | hypothetical protein | - |
| AM428_RS29325 (AM428_29120) | 107495..109360 | - | 1866 | WP_000178857 | conjugative transfer system coupling protein TraD | virb4 |
| AM428_RS29330 (AM428_29125) | 109357..112329 | - | 2973 | WP_001326173 | MobH family relaxase | - |
| AM428_RS29335 (AM428_29130) | 112497..113114 | + | 618 | WP_001249395 | hypothetical protein | - |
| AM428_RS29340 (AM428_29135) | 113096..113329 | + | 234 | WP_001191890 | hypothetical protein | - |
Host bacterium
| ID | 1362 | GenBank | NZ_CP018817 |
| Plasmid name | unitig_1-pAR_0049 | Incompatibility group | IncA/C2 |
| Plasmid size | 140825 bp | Coordinate of oriT [Strand] | 106944..107494 [-] |
| Host baterium | Klebsiella pneumoniae strain AR_0049 |
Cargo genes
| Drug resistance gene | blaNDM-1, rmtC, sul1, qacE, aac(6')-Ib, blaCMY-6 |
| Virulence gene | htpB |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |