Detailed information of oriT
oriT
The information of the oriT region
oriTDB ID | 100900 |
Name | oriT_pKp_Goe_641-2 |
Organism | Klebsiella pneumoniae strain Kp_Goe_121641 |
Sequence Completeness | core |
NCBI accession of oriT (coordinates [strand]) | NZ_CP018736 (42615..42720 [+], 106 nt) |
oriT length | 106 nt |
IRs (inverted repeats) | 18..35, 41..58 (GTTTTTGGAGTACCGCCG..CGGCGGCCGTCCAAAAAC) |
Location of nic site | _ |
Conserved sequence flanking the nic site |
_ |
Note | predicted by the oriTfinder |
oriT sequence
Download Length: 106 nt
>oriT_pKp_Goe_641-2
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure fileRelaxase
ID | 971 | GenBank | WP_074513113 |
Name | PutativerelaxaseofpKp_Goe_641-2 | UniProt ID | _ |
Length | 659 a.a. | PDB ID | |
Note | putative relaxase |
Relaxase protein sequence
Download Length: 659 a.a. Molecular weight: 75221.97 Da Isoelectric Point: 9.9948
>WP_074513113.1 TraI/MobA(P) family conjugative relaxase [Klebsiella pneumoniae]
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEVVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEVVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 45516..63589
Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
---|---|---|---|---|---|---|
BB745_RS28750 (BB745_05400) | 40600..40911 | + | 312 | WP_004187333 | hypothetical protein | - |
BB745_RS28755 (BB745_05401) | 41044..41580 | + | 537 | WP_004187332 | hypothetical protein | - |
BB745_RS28760 (BB745_05402) | 42082..42477 | - | 396 | WP_019725163 | hypothetical protein | - |
BB745_RS31490 (BB745_05403) | 42512..43039 | + | 528 | WP_172740549 | plasmid mobilization protein MobA | - |
BB745_RS28770 (BB745_05404) | 43026..45005 | + | 1980 | WP_074513113 | TraI/MobA(P) family conjugative relaxase | - |
BB745_RS28775 (BB745_05405) | 45019..45519 | + | 501 | WP_004187320 | DotD/TraH family lipoprotein | - |
BB745_RS28780 (BB745_05406) | 45516..46295 | + | 780 | WP_004187315 | type IV secretory system conjugative DNA transfer family protein | traI |
BB745_RS28785 (BB745_05407) | 46306..47469 | + | 1164 | WP_004187313 | plasmid transfer ATPase TraJ | virB11 |
BB745_RS28790 (BB745_05408) | 47459..47719 | + | 261 | WP_004187310 | IcmT/TraK family protein | traK |
BB745_RS31495 (BB745_05409) | 47744..51181 | + | 3438 | WP_015586048 | LPD7 domain-containing protein | - |
BB745_RS28800 (BB745_05410) | 51147..51659 | + | 513 | WP_011091071 | hypothetical protein | traL |
BB745_RS28805 | 51660..51872 | - | 213 | WP_305953721 | Hha/YmoA family nucleoid-associated regulatory protein | - |
BB745_RS28810 (BB745_05411) | 51844..52254 | - | 411 | WP_004187465 | H-NS family nucleoid-associated regulatory protein | - |
BB745_RS28815 (BB745_05412) | 52322..53035 | + | 714 | WP_004187467 | DotI/IcmL family type IV secretion protein | traM |
BB745_RS28820 (BB745_05413) | 53044..54195 | + | 1152 | WP_004187471 | DotH/IcmK family type IV secretion protein | traN |
BB745_RS28825 (BB745_05414) | 54207..55556 | + | 1350 | WP_004187474 | conjugal transfer protein TraO | traO |
BB745_RS28830 (BB745_05415) | 55568..56272 | + | 705 | WP_015060002 | conjugal transfer protein TraP | traP |
BB745_RS28835 (BB745_05416) | 56296..56826 | + | 531 | WP_004187478 | conjugal transfer protein TraQ | traQ |
BB745_RS28840 (BB745_05417) | 56843..57232 | + | 390 | WP_004187479 | DUF6750 family protein | traR |
BB745_RS28845 (BB745_05418) | 57278..57772 | + | 495 | WP_004187480 | hypothetical protein | - |
BB745_RS28850 (BB745_05419) | 57769..60819 | + | 3051 | WP_004187482 | hypothetical protein | traU |
BB745_RS28855 (BB745_05420) | 60816..62024 | + | 1209 | WP_011091082 | conjugal transfer protein TraW | traW |
BB745_RS28860 (BB745_05421) | 62021..62617 | + | 597 | WP_032419542 | hypothetical protein | - |
BB745_RS28865 | 62610..63589 | + | 980 | WP_148722743 | DotA/TraY family protein | traY |
Host bacterium
ID | 1359 | GenBank | NZ_CP018736 |
Plasmid name | pKp_Goe_641-2 | Incompatibility group | IncL/M |
Plasmid size | 63589 bp | Coordinate of oriT [Strand] | 42615..42720 [+] |
Host baterium | Klebsiella pneumoniae strain Kp_Goe_121641 |
Cargo genes
Drug resistance gene | blaOXA-48 |
Virulence gene | - |
Metal resistance gene | - |
Degradation gene | - |
Symbiosis gene | - |
Anti-CRISPR | - |