Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 100899 |
| Name | oriT_pKp_Goe_304-3  |
| Organism | Klebsiella pneumoniae strain KP_Goe_828304 |
| Sequence Completeness | core |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP018723 (55044..55149 [+], 106 nt) |
| oriT length | 106 nt |
| IRs (inverted repeats) | 18..35, 41..58 (GTTTTTGGAGTACCGCCG..CGGCGGCCGTCCAAAAAC) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | predicted by the oriTfinder |
oriT sequence
Download Length: 106 nt
>oriT_pKp_Goe_304-3
AGATAGCTAACCTCGTTAGGGGGTGTCGGGGCTTGCCCTGACCAAGACGTTTTTGGACGGCCGCCGCGTGTCGGCGGTACTCCAAAAACACATCTTGTCCCGTACT
AGATAGCTAACCTCGTTAGGGGGTGTCGGGGCTTGCCCTGACCAAGACGTTTTTGGACGGCCGCCGCGTGTCGGCGGTACTCCAAAAACACATCTTGTCCCGTACT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 970 | GenBank | WP_004187323 |
| Name | PutativerelaxaseofpKp_Goe_304-3 |
UniProt ID | _ |
| Length | 659 a.a. | PDB ID | |
| Note | putative relaxase | ||
Relaxase protein sequence
Download Length: 659 a.a. Molecular weight: 75179.89 Da Isoelectric Point: 9.9948
>WP_004187323.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 24951..52248
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| BB787_RS30270 (BB787_30270) | 20579..21376 | - | 798 | WP_015059991 | OXA-48 family carbapenem-hydrolyzing class D beta-lactamase OXA-48 | - |
| BB787_RS30285 (BB787_30285) | 21595..22292 | + | 698 | WP_095033700 | IS1-like element IS1B family transposase | - |
| BB787_RS30290 (BB787_30290) | 22289..23494 | + | 1206 | WP_025987686 | IS4-like element IS10A family transposase | - |
| BB787_RS30295 (BB787_30295) | 23509..23943 | - | 435 | Protein_39 | CPBP family intramembrane glutamate endopeptidase | - |
| BB787_RS30300 (BB787_30300) | 24023..24406 | + | 384 | WP_019725042 | DUF1496 domain-containing protein | - |
| BB787_RS30305 (BB787_30305) | 24511..24906 | + | 396 | WP_004187436 | lytic transglycosylase domain-containing protein | - |
| BB787_RS30310 (BB787_30310) | 24951..26213 | + | 1263 | WP_004206887 | hypothetical protein | trbA |
| BB787_RS30315 (BB787_30315) | 26224..27174 | + | 951 | WP_004206886 | DsbC family protein | trbB |
| BB787_RS30320 (BB787_30320) | 27187..29274 | + | 2088 | WP_004206885 | conjugal transfer protein TrbC | - |
| BB787_RS33655 | 29325..29420 | - | 96 | WP_004206884 | DinQ-like type I toxin DqlB | - |
| BB787_RS30325 (BB787_30325) | 30643..31698 | - | 1056 | WP_015060006 | plasmid replication initiator RepA | - |
| BB787_RS30330 (BB787_30330) | 31994..32224 | - | 231 | WP_011091085 | IncL/M type plasmid replication protein RepC | - |
| BB787_RS30335 (BB787_30335) | 32305..32958 | - | 654 | WP_015060005 | hypothetical protein | - |
| BB787_RS30340 (BB787_30340) | 32960..35155 | - | 2196 | WP_015062834 | DotA/TraY family protein | traY |
| BB787_RS30345 (BB787_30345) | 35148..35744 | - | 597 | WP_032419542 | hypothetical protein | - |
| BB787_RS30350 (BB787_30350) | 35741..36949 | - | 1209 | WP_011091082 | conjugal transfer protein TraW | traW |
| BB787_RS30355 (BB787_30355) | 36946..39996 | - | 3051 | WP_004187482 | hypothetical protein | traU |
| BB787_RS30360 (BB787_30360) | 39993..40487 | - | 495 | WP_004187480 | hypothetical protein | - |
| BB787_RS30365 (BB787_30365) | 40533..40922 | - | 390 | WP_004187479 | DUF6750 family protein | traR |
| BB787_RS30370 (BB787_30370) | 40939..41469 | - | 531 | WP_004187478 | conjugal transfer protein TraQ | traQ |
| BB787_RS30375 (BB787_30375) | 41493..42197 | - | 705 | WP_015060002 | conjugal transfer protein TraP | traP |
| BB787_RS30380 (BB787_30380) | 42209..43558 | - | 1350 | WP_004187474 | conjugal transfer protein TraO | traO |
| BB787_RS30385 (BB787_30385) | 43570..44721 | - | 1152 | WP_004187471 | DotH/IcmK family type IV secretion protein | traN |
| BB787_RS30390 (BB787_30390) | 44730..45443 | - | 714 | WP_004187467 | DotI/IcmL family type IV secretion protein | traM |
| BB787_RS30395 (BB787_30395) | 45511..45921 | + | 411 | WP_004187465 | H-NS family nucleoid-associated regulatory protein | - |
| BB787_RS30400 (BB787_30400) | 45950..46105 | + | 156 | WP_172693602 | Hha/YmoA family nucleoid-associated regulatory protein | - |
| BB787_RS30405 (BB787_30405) | 46106..46618 | - | 513 | WP_011091071 | hypothetical protein | traL |
| BB787_RS30410 (BB787_30410) | 46584..48260 | - | 1677 | WP_025987682 | LPD7 domain-containing protein | - |
| BB787_RS30415 (BB787_30415) | 48257..50020 | - | 1764 | WP_074115708 | toprim domain-containing protein | - |
| BB787_RS30420 (BB787_30420) | 50045..50305 | - | 261 | WP_004187310 | IcmT/TraK family protein | traK |
| BB787_RS30425 (BB787_30425) | 50295..51458 | - | 1164 | WP_004187313 | plasmid transfer ATPase TraJ | virB11 |
| BB787_RS30430 (BB787_30430) | 51469..52248 | - | 780 | WP_004187315 | type IV secretory system conjugative DNA transfer family protein | traI |
| BB787_RS30435 (BB787_30435) | 52245..52745 | - | 501 | WP_004187320 | DotD/TraH family lipoprotein | - |
| BB787_RS30440 (BB787_30440) | 52759..54738 | - | 1980 | WP_004187323 | TraI/MobA(P) family conjugative relaxase | - |
| BB787_RS33540 | 54725..55252 | - | 528 | WP_172740549 | plasmid mobilization protein MobA | - |
| BB787_RS30450 (BB787_30450) | 55287..55682 | + | 396 | WP_019725163 | hypothetical protein | - |
| BB787_RS30455 (BB787_30455) | 56184..56720 | - | 537 | WP_004187332 | hypothetical protein | - |
| BB787_RS30460 (BB787_30460) | 56853..57164 | - | 312 | WP_004187333 | hypothetical protein | - |
Host bacterium
| ID | 1358 | GenBank | NZ_CP018723 |
| Plasmid name | pKp_Goe_304-3 | Incompatibility group | IncL/M |
| Plasmid size | 63588 bp | Coordinate of oriT [Strand] | 55044..55149 [+] |
| Host baterium | Klebsiella pneumoniae strain KP_Goe_828304 |
Cargo genes
| Drug resistance gene | blaOXA-48 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrIIA8 |