Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 100898 |
| Name | oriT_pKp_Goe_021-3  |
| Organism | Klebsiella pneumoniae strain Kp_Goe_152021 |
| Sequence Completeness | core |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP018717 (57939..58044 [+], 106 nt) |
| oriT length | 106 nt |
| IRs (inverted repeats) | 18..35, 41..58 (GTTTTTGGAGTACCGCCG..CGGCGGCCGTCCAAAAAC) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | predicted by the oriTfinder |
oriT sequence
Download Length: 106 nt
>oriT_pKp_Goe_021-3
AGATAGCTAACCTCGTTAGGGGGTGTCGGGGCTTGCCCTGACCAAGACGTTTTTGGACGGCCGCCGCGTGTCGGCGGTACTCCAAAAACACATCTTGTCCCGTACT
AGATAGCTAACCTCGTTAGGGGGTGTCGGGGCTTGCCCTGACCAAGACGTTTTTGGACGGCCGCCGCGTGTCGGCGGTACTCCAAAAACACATCTTGTCCCGTACT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 969 | GenBank | WP_004187323 |
| Name | PutativerelaxaseofpKp_Goe_021-3 |
UniProt ID | _ |
| Length | 659 a.a. | PDB ID | |
| Note | putative relaxase | ||
Relaxase protein sequence
Download Length: 659 a.a. Molecular weight: 75179.89 Da Isoelectric Point: 9.9948
>WP_004187323.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 27800..55143
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| BB786_RS30280 (BB786_30280) | 23473..24270 | - | 798 | WP_015059991 | OXA-48 family carbapenem-hydrolyzing class D beta-lactamase OXA-48 | - |
| BB786_RS30295 (BB786_30295) | 24489..25186 | + | 698 | WP_095033700 | IS1-like element IS1B family transposase | - |
| BB786_RS30300 (BB786_30300) | 25183..26388 | + | 1206 | WP_025987686 | IS4-like element IS10A family transposase | - |
| BB786_RS30305 (BB786_30305) | 26403..26837 | - | 435 | Protein_42 | CPBP family intramembrane glutamate endopeptidase | - |
| BB786_RS30310 (BB786_30310) | 26917..27300 | + | 384 | WP_019725042 | DUF1496 domain-containing protein | - |
| BB786_RS30315 (BB786_30315) | 27405..27800 | + | 396 | WP_004187436 | lytic transglycosylase domain-containing protein | - |
| BB786_RS30320 (BB786_30320) | 27800..29107 | + | 1308 | WP_015059988 | hypothetical protein | trbA |
| BB786_RS30325 (BB786_30325) | 29118..30068 | + | 951 | WP_004206886 | DsbC family protein | trbB |
| BB786_RS30330 (BB786_30330) | 30081..32168 | + | 2088 | WP_004206885 | conjugal transfer protein TrbC | - |
| BB786_RS33570 | 32219..32314 | - | 96 | WP_004206884 | DinQ-like type I toxin DqlB | - |
| BB786_RS30335 (BB786_30335) | 33537..34592 | - | 1056 | WP_015060006 | plasmid replication initiator RepA | - |
| BB786_RS30340 (BB786_30340) | 34888..35118 | - | 231 | WP_011091085 | IncL/M type plasmid replication protein RepC | - |
| BB786_RS30345 (BB786_30345) | 35199..35852 | - | 654 | WP_015060005 | hypothetical protein | - |
| BB786_RS30350 (BB786_30350) | 35854..38049 | - | 2196 | WP_015062834 | DotA/TraY family protein | traY |
| BB786_RS30355 (BB786_30355) | 38042..38638 | - | 597 | WP_032419542 | hypothetical protein | - |
| BB786_RS30360 (BB786_30360) | 38635..39843 | - | 1209 | WP_011091082 | conjugal transfer protein TraW | traW |
| BB786_RS30365 (BB786_30365) | 39840..42890 | - | 3051 | WP_004187482 | hypothetical protein | traU |
| BB786_RS30370 (BB786_30370) | 42887..43381 | - | 495 | WP_004187480 | hypothetical protein | - |
| BB786_RS30375 (BB786_30375) | 43427..43816 | - | 390 | WP_004187479 | DUF6750 family protein | traR |
| BB786_RS30380 (BB786_30380) | 43833..44363 | - | 531 | WP_004187478 | conjugal transfer protein TraQ | traQ |
| BB786_RS30385 (BB786_30385) | 44387..45091 | - | 705 | WP_015060002 | conjugal transfer protein TraP | traP |
| BB786_RS30390 (BB786_30390) | 45103..46452 | - | 1350 | WP_004187474 | conjugal transfer protein TraO | traO |
| BB786_RS30395 (BB786_30395) | 46464..47615 | - | 1152 | WP_004187471 | DotH/IcmK family type IV secretion protein | traN |
| BB786_RS30400 (BB786_30400) | 47624..48337 | - | 714 | WP_004187467 | DotI/IcmL family type IV secretion protein | traM |
| BB786_RS30405 (BB786_30405) | 48405..48815 | + | 411 | WP_004187465 | H-NS family nucleoid-associated regulatory protein | - |
| BB786_RS30410 (BB786_30410) | 48844..48999 | + | 156 | WP_172693602 | Hha/YmoA family nucleoid-associated regulatory protein | - |
| BB786_RS30415 (BB786_30415) | 49000..49512 | - | 513 | WP_011091071 | hypothetical protein | traL |
| BB786_RS33460 | 49478..52915 | - | 3438 | WP_015586048 | LPD7 domain-containing protein | - |
| BB786_RS30425 (BB786_30425) | 52940..53200 | - | 261 | WP_004187310 | IcmT/TraK family protein | traK |
| BB786_RS30430 (BB786_30430) | 53190..54353 | - | 1164 | WP_004187313 | plasmid transfer ATPase TraJ | virB11 |
| BB786_RS30435 (BB786_30435) | 54364..55143 | - | 780 | WP_004187315 | type IV secretory system conjugative DNA transfer family protein | traI |
| BB786_RS30440 (BB786_30440) | 55140..55640 | - | 501 | WP_004187320 | DotD/TraH family lipoprotein | - |
| BB786_RS30445 (BB786_30445) | 55654..57633 | - | 1980 | WP_004187323 | TraI/MobA(P) family conjugative relaxase | - |
| BB786_RS33465 | 57620..58147 | - | 528 | WP_172740549 | plasmid mobilization protein MobA | - |
| BB786_RS30455 (BB786_30455) | 58182..58577 | + | 396 | WP_019725163 | hypothetical protein | - |
| BB786_RS30460 (BB786_30460) | 59079..59615 | - | 537 | WP_004187332 | hypothetical protein | - |
| BB786_RS30465 (BB786_30465) | 59748..60059 | - | 312 | WP_004187333 | hypothetical protein | - |
Host bacterium
| ID | 1357 | GenBank | NZ_CP018717 |
| Plasmid name | pKp_Goe_021-3 | Incompatibility group | IncL/M |
| Plasmid size | 63589 bp | Coordinate of oriT [Strand] | 57939..58044 [+] |
| Host baterium | Klebsiella pneumoniae strain Kp_Goe_152021 |
Cargo genes
| Drug resistance gene | blaOXA-48 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |