Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 100897 |
| Name | oriT_pKp_Goe_026-3  |
| Organism | Klebsiella pneumoniae strain Kp_Goe_827026 |
| Sequence Completeness | core |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP018712 (61157..61262 [+], 106 nt) |
| oriT length | 106 nt |
| IRs (inverted repeats) | 18..35, 41..58 (GTTTTTGGAGTACCGCCG..CGGCGGCCGTCCAAAAAC) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | predicted by the oriTfinder |
oriT sequence
Download Length: 106 nt
>oriT_pKp_Goe_026-3
AGATAGCTAACCTCGTTAGGGGGTGTCGGGGCTTGCCCTGACCAAGACGTTTTTGGACGGCCGCCGCGTGTCGGCGGTACTCCAAAAACACATCTTGTCCCGTACT
AGATAGCTAACCTCGTTAGGGGGTGTCGGGGCTTGCCCTGACCAAGACGTTTTTGGACGGCCGCCGCGTGTCGGCGGTACTCCAAAAACACATCTTGTCCCGTACT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 968 | GenBank | WP_004187323 |
| Name | PutativerelaxaseofpKp_Goe_026-3 |
UniProt ID | _ |
| Length | 659 a.a. | PDB ID | |
| Note | putative relaxase | ||
Relaxase protein sequence
Download Length: 659 a.a. Molecular weight: 75179.89 Da Isoelectric Point: 9.9948
>WP_004187323.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 31018..58361
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| BB785_RS30730 (BB785_30730) | 26691..27488 | - | 798 | Protein_46 | OXA-48 family class D beta-lactamase | - |
| BB785_RS30745 (BB785_30745) | 27707..28404 | + | 698 | WP_095033700 | IS1-like element IS1B family transposase | - |
| BB785_RS30750 (BB785_30750) | 28401..29606 | + | 1206 | WP_025987686 | IS4-like element IS10A family transposase | - |
| BB785_RS30755 (BB785_30755) | 29621..30055 | - | 435 | Protein_49 | CPBP family intramembrane glutamate endopeptidase | - |
| BB785_RS30760 (BB785_30760) | 30135..30518 | + | 384 | WP_019725042 | DUF1496 domain-containing protein | - |
| BB785_RS30765 (BB785_30765) | 30623..31018 | + | 396 | WP_004187436 | lytic transglycosylase domain-containing protein | - |
| BB785_RS30770 (BB785_30770) | 31018..32325 | + | 1308 | WP_015059988 | hypothetical protein | trbA |
| BB785_RS30775 (BB785_30775) | 32336..33286 | + | 951 | WP_004206886 | DsbC family protein | trbB |
| BB785_RS30780 (BB785_30780) | 33299..35386 | + | 2088 | WP_004206885 | conjugal transfer protein TrbC | - |
| BB785_RS33695 | 35437..35532 | - | 96 | WP_004206884 | DinQ-like type I toxin DqlB | - |
| BB785_RS30785 (BB785_30785) | 36755..37810 | - | 1056 | WP_015060006 | plasmid replication initiator RepA | - |
| BB785_RS30790 (BB785_30790) | 38106..38336 | - | 231 | WP_011091085 | IncL/M type plasmid replication protein RepC | - |
| BB785_RS30795 (BB785_30795) | 38417..39070 | - | 654 | WP_015060005 | hypothetical protein | - |
| BB785_RS30800 (BB785_30800) | 39072..41267 | - | 2196 | WP_015062834 | DotA/TraY family protein | traY |
| BB785_RS30805 (BB785_30805) | 41260..41856 | - | 597 | WP_032419542 | hypothetical protein | - |
| BB785_RS30810 (BB785_30810) | 41853..43061 | - | 1209 | WP_011091082 | conjugal transfer protein TraW | traW |
| BB785_RS30815 (BB785_30815) | 43058..46108 | - | 3051 | WP_004187482 | hypothetical protein | traU |
| BB785_RS30820 (BB785_30820) | 46105..46599 | - | 495 | WP_004187480 | hypothetical protein | - |
| BB785_RS30825 (BB785_30825) | 46645..47034 | - | 390 | WP_004187479 | DUF6750 family protein | traR |
| BB785_RS30830 (BB785_30830) | 47051..47581 | - | 531 | WP_004187478 | conjugal transfer protein TraQ | traQ |
| BB785_RS30835 (BB785_30835) | 47605..48309 | - | 705 | WP_015060002 | conjugal transfer protein TraP | traP |
| BB785_RS30840 (BB785_30840) | 48321..49670 | - | 1350 | WP_004187474 | conjugal transfer protein TraO | traO |
| BB785_RS30845 (BB785_30845) | 49682..50833 | - | 1152 | WP_004187471 | DotH/IcmK family type IV secretion protein | traN |
| BB785_RS30850 (BB785_30850) | 50842..51555 | - | 714 | WP_004187467 | DotI/IcmL family type IV secretion protein | traM |
| BB785_RS30855 (BB785_30855) | 51623..52033 | + | 411 | WP_004187465 | H-NS family nucleoid-associated regulatory protein | - |
| BB785_RS30860 (BB785_30860) | 52062..52217 | + | 156 | WP_172693602 | Hha/YmoA family nucleoid-associated regulatory protein | - |
| BB785_RS30865 (BB785_30865) | 52218..52730 | - | 513 | WP_011091071 | hypothetical protein | traL |
| BB785_RS33585 | 52696..56133 | - | 3438 | WP_015586048 | LPD7 domain-containing protein | - |
| BB785_RS30875 (BB785_30875) | 56158..56418 | - | 261 | WP_004187310 | IcmT/TraK family protein | traK |
| BB785_RS30880 (BB785_30880) | 56408..57571 | - | 1164 | WP_004187313 | plasmid transfer ATPase TraJ | virB11 |
| BB785_RS30885 (BB785_30885) | 57582..58361 | - | 780 | WP_004187315 | type IV secretory system conjugative DNA transfer family protein | traI |
| BB785_RS30890 (BB785_30890) | 58358..58858 | - | 501 | WP_004187320 | DotD/TraH family lipoprotein | - |
| BB785_RS30895 (BB785_30895) | 58872..60851 | - | 1980 | WP_004187323 | TraI/MobA(P) family conjugative relaxase | - |
| BB785_RS33590 | 60838..61365 | - | 528 | WP_172740549 | plasmid mobilization protein MobA | - |
| BB785_RS30905 (BB785_30905) | 61400..61795 | + | 396 | WP_019725163 | hypothetical protein | - |
| BB785_RS30910 (BB785_30910) | 62297..62833 | - | 537 | WP_004187332 | hypothetical protein | - |
| BB785_RS30915 (BB785_30915) | 62966..63277 | - | 312 | WP_004187333 | hypothetical protein | - |
Host bacterium
| ID | 1356 | GenBank | NZ_CP018712 |
| Plasmid name | pKp_Goe_026-3 | Incompatibility group | IncL/M |
| Plasmid size | 63583 bp | Coordinate of oriT [Strand] | 61157..61262 [+] |
| Host baterium | Klebsiella pneumoniae strain Kp_Goe_827026 |
Cargo genes
| Drug resistance gene | blaOXA-48 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |