Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100895
Name   oriT_pKp_Goe_024-2 in_silico
Organism   Klebsiella pneumoniae strain Kp_Goe_827024
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NZ_CP018704 (1..549 [-], 549 nt)
oriT length   549 nt
IRs (inverted repeats)      IR1: 62..70, 71..79  (AACCCTTTC..GACAGGGTT)
  IR2: 143..150, 154..161  (TGGCCTGC..GGAGGCCA)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 549 nt

>oriT_pKp_Goe_024-2
GAGCAGAGCTATGTGTGACAAGAAGTATAGAAATTACGAGGTAGCCATCATGGTCGATGTGAACCCTTTCGACAGGGTTATGAATGAATTGAAAAGTCGTGGCCGCAAGAACGCTCACATCCTGAGCATCCTCCAATTCGACTGGCCTGCATCGGAGGCCATCATCGAGAAGCTGAGCTGCTACATCACAGACGGGATTAAGGCTAATCAGGAGCCTGTGATTTACCCGATCATTGAAGAAGCTCTGCATCGCTACAGCCAGCTCGTGTTTCATGAGCAGAGAGAGAAATATGAAGACCCGGCCAGAATTGGGGCATTTCTGGAAACCCTGATCACCGAAACCTGCCGGGCGTTGGAAGTGCAAATTGTCGATAGTGGCGGTGATTCATGGTCTGTCGATTCAGGAGAGTCGTTCTCACTGTGGCTTTCTTCCCATCCAGGAGAACTATCCATTAACCCGCAGCCCCATGAGGATGAGACCTCTTTGCGTGGCTTGCTGTATGAGCTCATCACCTGTGAGAGCGTGAAAACTGTTTTAAGGAGAACCGA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   966 GenBank   WP_001326173
Name   TraI_pKp_Goe_024-2 insolico UniProt ID   _
Length   990 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 990 a.a.        Molecular weight: 109356.90 Da        Isoelectric Point: 4.9395

>WP_001326173.1 MULTISPECIES: MobH family relaxase [Gammaproteobacteria]
MLKALNKLFGGRSGVIETAPSVRVLPLKDVEDEEIPRYPPFAKGLPVAPLDKILATQAELIEKVRNSLGF
TVDDFNRLVLPVIQRYAAFVHLLPASESHHHRGAGGLFRHGLEVAFWAAQASESVIFSIEGTPRERRDNE
PRWRLASCFSGLLHDVGKPLSDVSITDKDGSITWNPYSESLHDWAHRHEIDRYFIRWRDKRHKRHEQFSL
LAVDRIIPAETREFLSKSGPSIMEAMLEAISGTSVNQPVTKLMLRADQESVSRDLRQSRLDVDEFSYGVP
VERYVFDAIRRLVKTGKWKVNEPGAKVWHLNQGVFIAWKQLGDLYDLISHDKIPGIPRDPDTLADILIER
GFAVPNTVQEKGERAYYRYWEVLPEMLQEAAGSVKILMLRLESNDLVFTTEPPAAVAAEVVGDVEDAEIE
FVDPEEVDDDQEEDVSALNDDMLAAEQEAEKALAGLGFGDAMEMLKSTSDAVEEKPEQKDAGSTESSKPD
AGKKGKPQSKPGKAKPKSDTEKQPHKPEAKEDLSPQDIAKNAPPLANDNPLQALKDVGGGLGDIDFPFDA
FSASAETASTDATNSEIPDVAMPGKQEKQPKQDFVPQEQNSLQGDDFPMFGSSDEPPSWAIEPLPMLTDA
PEQTTPAPAMPPTDKPNLHEKDAKTLLVEMLAGYGEASALLEQAIMPVLEGKTTLGEVLCLMKGQAVILY
PDGARSLGAPSEVLSKLSHANAIVPDPIMPGRKVRDFSGVKAIVLAEQLSDAVVAAIKDAEASMGGYQDA
FELVSPPGLDASKNKSAPKQQSRKKAQQQKPEVNAGKPSPEQKAKGKDSQPQQKEKKVDVTSPVEEPQRQ
PVQEKQNVARLPKREVQPVAPEPKVEREKELGHVEVREREEPEVREFEPPKAKTNPKDINAEDFLPSGVT
PQKALQMLKDMIQKRSGRWLVTPVLEEDGCLVTSDKAFDMIAGENIGISKHILCGMLSRAQRRPLLKKRQ
GKLYLEVNET

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(51-357)


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 89398..96071

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BB784_RS33460 85024..85221 + 198 Protein_109 mercuric resistance transcriptional repressor MerD -
BB784_RS29975 (BB784_29975) 85275..85979 - 705 WP_001067855 IS6-like element IS26 family transposase -
BB784_RS29980 (BB784_29980) 86044..86217 + 174 Protein_111 transcriptional regulator MerD -
BB784_RS29985 (BB784_29985) 86214..86450 + 237 WP_001087810 broad-spectrum mercury transporter MerE -
BB784_RS29990 (BB784_29990) 86486..87121 + 636 Protein_113 EAL domain-containing protein -
BB784_RS33465 87233..87436 + 204 WP_024198696 DUF3330 domain-containing protein -
BB784_RS29995 (BB784_29995) 87562..88098 + 537 WP_029404405 recombinase family protein -
BB784_RS30010 (BB784_30010) 88149..88846 + 698 WP_095033700 IS1-like element IS1B family transposase -
BB784_RS30015 (BB784_30015) 89002..89394 - 393 WP_000479535 TraA family conjugative transfer protein -
BB784_RS30020 (BB784_30020) 89398..89976 - 579 WP_000793435 type IV conjugative transfer system lipoprotein TraV traV
BB784_RS30025 (BB784_30025) 89973..91286 - 1314 WP_024131605 TraB/VirB10 family protein traB
BB784_RS30030 (BB784_30030) 91286..92203 - 918 WP_000794249 type-F conjugative transfer system secretin TraK traK
BB784_RS30035 (BB784_30035) 92187..92813 - 627 WP_001049717 TraE/TraK family type IV conjugative transfer system protein traE
BB784_RS30040 (BB784_30040) 92810..93091 - 282 WP_000805625 type IV conjugative transfer system protein TraL traL
BB784_RS30045 (BB784_30045) 93236..93601 - 366 WP_001052530 hypothetical protein -
BB784_RS33190 93613..93756 - 144 WP_001275801 hypothetical protein -
BB784_RS30050 (BB784_30050) 93937..94599 - 663 WP_001231464 hypothetical protein -
BB784_RS30055 (BB784_30055) 94599..94976 - 378 WP_000869297 hypothetical protein -
BB784_RS30060 (BB784_30060) 94986..95432 - 447 WP_000122507 hypothetical protein -
BB784_RS30065 (BB784_30065) 95442..96071 - 630 WP_000743449 DUF4400 domain-containing protein tfc7

Host bacterium

ID   1354 GenBank   NZ_CP018704
Plasmid name   pKp_Goe_024-2 Incompatibility group   IncA/C2
Plasmid size   96073 bp Coordinate of oriT [Strand]   1..549 [-]
Host baterium   Klebsiella pneumoniae strain Kp_Goe_827024

Cargo genes

Drug resistance gene   sul2, aph(3'')-Ib, aph(6)-Id, tet(A), floR, sul1, qacE, aac(6')-Ib
Virulence gene   -
Metal resistance gene   merR, merT, merP, merA, merB, merE
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -