Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100893
Name   oriT_pKp_Goe_832-2 in_silico
Organism   Klebsiella pneumoniae strain Kp_Goe_149832
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NZ_CP018698 (9588..10138 [-], 551 nt)
oriT length   551 nt
IRs (inverted repeats)      IR1: 62..70, 71..79  (AACCCTTTC..GACAGGGTT)
  IR2: 143..150, 154..161  (TGGCCTGC..GGAGGCCA)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 551 nt

>oriT_pKp_Goe_832-2
GAGCAGAGCTATGTGTGACAAGAAGTATAGAAATTACGAGGTAGCCATCATGGTCGATGTGAACCCTTTCGACAGGGTTATGAATGAATTGAAAAGTCGTGGCCGCAAGAACGCTCACATCCTGAGCATCCTCCAATTCGACTGGCCTGCATCGGAGGCCATCATCGAGAAGCTGAGCTGCTACATCACAGACGGGATTAAGGCTAATCAGGAGCCTGTGATTTACCCGATCATTGAAGAAGCTCTGCATCGCTACAGCCAGCTCGTGTTTCATGAGCAGAGAGAGAAATATGAAGACCCGGCCAGAATTGGGGCATTTCTGGAAACCCTGATCACCGAAACCTGCCGGGCGTTGGAAGTGCAAATTGTCGATAGTGGCGGTGATTCATGGTCTGTCGATTCAGGAGAGTCGTTCTCACTGTGGCTTTCTTCCCATCCAGGAGAACTATCCATTAACCCGCAGCCCCATGAGGATGAGACCTCTTTGCGTGGCTTGCTGTATGAGCTCATCACCTGTGAGAGCGTGAAAACTGTTTTAAGGAGAACCGACT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   964 GenBank   WP_001326173
Name   TraI_pKp_Goe_832-2 insolico UniProt ID   _
Length   990 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 990 a.a.        Molecular weight: 109356.90 Da        Isoelectric Point: 4.9395

>WP_001326173.1 MULTISPECIES: MobH family relaxase [Gammaproteobacteria]
MLKALNKLFGGRSGVIETAPSVRVLPLKDVEDEEIPRYPPFAKGLPVAPLDKILATQAELIEKVRNSLGF
TVDDFNRLVLPVIQRYAAFVHLLPASESHHHRGAGGLFRHGLEVAFWAAQASESVIFSIEGTPRERRDNE
PRWRLASCFSGLLHDVGKPLSDVSITDKDGSITWNPYSESLHDWAHRHEIDRYFIRWRDKRHKRHEQFSL
LAVDRIIPAETREFLSKSGPSIMEAMLEAISGTSVNQPVTKLMLRADQESVSRDLRQSRLDVDEFSYGVP
VERYVFDAIRRLVKTGKWKVNEPGAKVWHLNQGVFIAWKQLGDLYDLISHDKIPGIPRDPDTLADILIER
GFAVPNTVQEKGERAYYRYWEVLPEMLQEAAGSVKILMLRLESNDLVFTTEPPAAVAAEVVGDVEDAEIE
FVDPEEVDDDQEEDVSALNDDMLAAEQEAEKALAGLGFGDAMEMLKSTSDAVEEKPEQKDAGSTESSKPD
AGKKGKPQSKPGKAKPKSDTEKQPHKPEAKEDLSPQDIAKNAPPLANDNPLQALKDVGGGLGDIDFPFDA
FSASAETASTDATNSEIPDVAMPGKQEKQPKQDFVPQEQNSLQGDDFPMFGSSDEPPSWAIEPLPMLTDA
PEQTTPAPAMPPTDKPNLHEKDAKTLLVEMLAGYGEASALLEQAIMPVLEGKTTLGEVLCLMKGQAVILY
PDGARSLGAPSEVLSKLSHANAIVPDPIMPGRKVRDFSGVKAIVLAEQLSDAVVAAIKDAEASMGGYQDA
FELVSPPGLDASKNKSAPKQQSRKKAQQQKPEVNAGKPSPEQKAKGKDSQPQQKEKKVDVTSPVEEPQRQ
PVQEKQNVARLPKREVQPVAPEPKVEREKELGHVEVREREEPEVREFEPPKAKTNPKDINAEDFLPSGVT
PQKALQMLKDMIQKRSGRWLVTPVLEEDGCLVTSDKAFDMIAGENIGISKHILCGMLSRAQRRPLLKKRQ
GKLYLEVNET

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(51-357)


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 2914..12004

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BB751_RS33460 749..952 + 204 WP_024198696 DUF3330 domain-containing protein -
BB751_RS29375 (BB751_29375) 1078..1614 + 537 WP_029404405 recombinase family protein -
BB751_RS29390 (BB751_29390) 1665..2362 + 698 WP_095033700 IS1-like element IS1B family transposase -
BB751_RS29395 (BB751_29395) 2518..2910 - 393 WP_000479535 TraA family conjugative transfer protein -
BB751_RS29400 (BB751_29400) 2914..3492 - 579 WP_000793435 type IV conjugative transfer system lipoprotein TraV traV
BB751_RS29405 (BB751_29405) 3489..4802 - 1314 WP_024131605 TraB/VirB10 family protein traB
BB751_RS29410 (BB751_29410) 4802..5719 - 918 WP_000794249 type-F conjugative transfer system secretin TraK traK
BB751_RS29415 (BB751_29415) 5703..6329 - 627 WP_001049717 TraE/TraK family type IV conjugative transfer system protein traE
BB751_RS29420 (BB751_29420) 6326..6607 - 282 WP_000805625 type IV conjugative transfer system protein TraL traL
BB751_RS29425 (BB751_29425) 6752..7117 - 366 WP_001052530 hypothetical protein -
BB751_RS33180 7129..7272 - 144 WP_001275801 hypothetical protein -
BB751_RS29430 (BB751_29430) 7453..8115 - 663 WP_001231464 hypothetical protein -
BB751_RS29435 (BB751_29435) 8115..8492 - 378 WP_000869297 hypothetical protein -
BB751_RS29440 (BB751_29440) 8502..8948 - 447 WP_000122507 hypothetical protein -
BB751_RS29445 (BB751_29445) 8958..9587 - 630 WP_000743449 DUF4400 domain-containing protein tfc7
BB751_RS29450 (BB751_29450) 9544..10089 - 546 WP_000228720 hypothetical protein -
BB751_RS29455 (BB751_29455) 10139..12004 - 1866 WP_000178857 conjugative transfer system coupling protein TraD virb4
BB751_RS29460 (BB751_29460) 12001..14973 - 2973 WP_001326173 MobH family relaxase -
BB751_RS29465 (BB751_29465) 15141..15758 + 618 WP_001249395 hypothetical protein -
BB751_RS29470 (BB751_29470) 15740..15973 + 234 WP_001191890 hypothetical protein -

Host bacterium

ID   1352 GenBank   NZ_CP018698
Plasmid name   pKp_Goe_832-2 Incompatibility group   IncA/C2
Plasmid size   96072 bp Coordinate of oriT [Strand]   9588..10138 [-]
Host baterium   Klebsiella pneumoniae strain Kp_Goe_149832

Cargo genes

Drug resistance gene   sul2, aph(3'')-Ib, aph(6)-Id, tet(A), floR, sul1, qacE, aac(6')-Ib
Virulence gene   -
Metal resistance gene   merR, merT, merP, merA, merB, merE
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -