Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 100892 |
| Name | oriT_pKp_Goe_473-3  |
| Organism | Klebsiella pneumoniae strain Kp_Goe_149473 |
| Sequence Completeness | core |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP018690 (56531..56636 [+], 106 nt) |
| oriT length | 106 nt |
| IRs (inverted repeats) | 18..35, 41..58 (GTTTTTGGAGTACCGCCG..CGGCGGCCGTCCAAAAAC) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | predicted by the oriTfinder |
oriT sequence
Download Length: 106 nt
>oriT_pKp_Goe_473-3
AGATAGCTAACCTCGTTAGGGGGTGTCGGGGCTTGCCCTGACCAAGACGTTTTTGGACGGCCGCCGCGTGTCGGCGGTACTCCAAAAACACATCTTGTCCCGTACT
AGATAGCTAACCTCGTTAGGGGGTGTCGGGGCTTGCCCTGACCAAGACGTTTTTGGACGGCCGCCGCGTGTCGGCGGTACTCCAAAAACACATCTTGTCCCGTACT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 963 | GenBank | WP_004187323 |
| Name | PutativerelaxaseofpKp_Goe_473-3 |
UniProt ID | _ |
| Length | 659 a.a. | PDB ID | |
| Note | putative relaxase | ||
Relaxase protein sequence
Download Length: 659 a.a. Molecular weight: 75179.89 Da Isoelectric Point: 9.9948
>WP_004187323.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 26392..53735
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| BB750_RS30270 (BB750_30270) | 22065..22862 | - | 798 | WP_015059991 | OXA-48 family carbapenem-hydrolyzing class D beta-lactamase OXA-48 | - |
| BB750_RS30285 (BB750_30285) | 23081..23778 | + | 698 | WP_095033700 | IS1-like element IS1B family transposase | - |
| BB750_RS30290 (BB750_30290) | 23775..24980 | + | 1206 | WP_025987686 | IS4-like element IS10A family transposase | - |
| BB750_RS30295 (BB750_30295) | 24995..25429 | - | 435 | Protein_40 | CPBP family intramembrane glutamate endopeptidase | - |
| BB750_RS30300 (BB750_30300) | 25509..25892 | + | 384 | WP_019725042 | DUF1496 domain-containing protein | - |
| BB750_RS30305 (BB750_30305) | 25997..26392 | + | 396 | WP_004187436 | lytic transglycosylase domain-containing protein | - |
| BB750_RS30310 (BB750_30310) | 26392..27699 | + | 1308 | WP_015059988 | hypothetical protein | trbA |
| BB750_RS30315 (BB750_30315) | 27710..28660 | + | 951 | WP_004206886 | DsbC family protein | trbB |
| BB750_RS30320 (BB750_30320) | 28673..30760 | + | 2088 | WP_004206885 | conjugal transfer protein TrbC | - |
| BB750_RS33545 | 30811..30906 | - | 96 | WP_004206884 | DinQ-like type I toxin DqlB | - |
| BB750_RS30325 (BB750_30325) | 32129..33184 | - | 1056 | WP_015060006 | plasmid replication initiator RepA | - |
| BB750_RS30330 (BB750_30330) | 33480..33710 | - | 231 | WP_011091085 | IncL/M type plasmid replication protein RepC | - |
| BB750_RS30335 (BB750_30335) | 33791..34444 | - | 654 | WP_015060005 | hypothetical protein | - |
| BB750_RS30340 (BB750_30340) | 34446..36641 | - | 2196 | WP_015062834 | DotA/TraY family protein | traY |
| BB750_RS30345 (BB750_30345) | 36634..37230 | - | 597 | WP_032419542 | hypothetical protein | - |
| BB750_RS30350 (BB750_30350) | 37227..38435 | - | 1209 | WP_011091082 | conjugal transfer protein TraW | traW |
| BB750_RS30355 (BB750_30355) | 38432..41482 | - | 3051 | WP_004187482 | hypothetical protein | traU |
| BB750_RS30360 (BB750_30360) | 41479..41973 | - | 495 | WP_004187480 | hypothetical protein | - |
| BB750_RS30365 (BB750_30365) | 42019..42408 | - | 390 | WP_004187479 | DUF6750 family protein | traR |
| BB750_RS30370 (BB750_30370) | 42425..42955 | - | 531 | WP_004187478 | conjugal transfer protein TraQ | traQ |
| BB750_RS30375 (BB750_30375) | 42979..43683 | - | 705 | WP_015060002 | conjugal transfer protein TraP | traP |
| BB750_RS30380 (BB750_30380) | 43695..45044 | - | 1350 | WP_004187474 | conjugal transfer protein TraO | traO |
| BB750_RS30385 (BB750_30385) | 45056..46207 | - | 1152 | WP_004187471 | DotH/IcmK family type IV secretion protein | traN |
| BB750_RS30390 (BB750_30390) | 46216..46929 | - | 714 | WP_004187467 | DotI/IcmL family type IV secretion protein | traM |
| BB750_RS30395 (BB750_30395) | 46997..47407 | + | 411 | WP_004187465 | H-NS family nucleoid-associated regulatory protein | - |
| BB750_RS30400 (BB750_30400) | 47436..47591 | + | 156 | WP_172693602 | Hha/YmoA family nucleoid-associated regulatory protein | - |
| BB750_RS30405 (BB750_30405) | 47592..48104 | - | 513 | WP_011091071 | hypothetical protein | traL |
| BB750_RS33435 | 48070..51507 | - | 3438 | WP_015586048 | LPD7 domain-containing protein | - |
| BB750_RS30415 (BB750_30415) | 51532..51792 | - | 261 | WP_004187310 | IcmT/TraK family protein | traK |
| BB750_RS30420 (BB750_30420) | 51782..52945 | - | 1164 | WP_004187313 | plasmid transfer ATPase TraJ | virB11 |
| BB750_RS30425 (BB750_30425) | 52956..53735 | - | 780 | WP_004187315 | type IV secretory system conjugative DNA transfer family protein | traI |
| BB750_RS30430 (BB750_30430) | 53732..54232 | - | 501 | WP_004187320 | DotD/TraH family lipoprotein | - |
| BB750_RS30435 (BB750_30435) | 54246..56225 | - | 1980 | WP_004187323 | TraI/MobA(P) family conjugative relaxase | - |
| BB750_RS33440 | 56212..56739 | - | 528 | WP_172740549 | plasmid mobilization protein MobA | - |
| BB750_RS30445 (BB750_30445) | 56774..57169 | + | 396 | WP_019725163 | hypothetical protein | - |
| BB750_RS30450 (BB750_30450) | 57671..58207 | - | 537 | WP_004187332 | hypothetical protein | - |
| BB750_RS30455 (BB750_30455) | 58340..58651 | - | 312 | WP_004187333 | hypothetical protein | - |
Host bacterium
| ID | 1351 | GenBank | NZ_CP018690 |
| Plasmid name | pKp_Goe_473-3 | Incompatibility group | IncL/M |
| Plasmid size | 63589 bp | Coordinate of oriT [Strand] | 56531..56636 [+] |
| Host baterium | Klebsiella pneumoniae strain Kp_Goe_149473 |
Cargo genes
| Drug resistance gene | blaOXA-48 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |