Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100891
Name   oriT_pKp_Goe_473-2 in_silico
Organism   Klebsiella pneumoniae strain Kp_Goe_149473
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NZ_CP018689 (24998..25548 [-], 551 nt)
oriT length   551 nt
IRs (inverted repeats)      IR1: 62..70, 71..79  (AACCCTTTC..GACAGGGTT)
  IR2: 143..150, 154..161  (TGGCCTGC..GGAGGCCA)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 551 nt

>oriT_pKp_Goe_473-2
GAGCAGAGCTATGTGTGACAAGAAGTATAGAAATTACGAGGTAGCCATCATGGTCGATGTGAACCCTTTCGACAGGGTTATGAATGAATTGAAAAGTCGTGGCCGCAAGAACGCTCACATCCTGAGCATCCTCCAATTCGACTGGCCTGCATCGGAGGCCATCATCGAGAAGCTGAGCTGCTACATCACAGACGGGATTAAGGCTAATCAGGAGCCTGTGATTTACCCGATCATTGAAGAAGCTCTGCATCGCTACAGCCAGCTCGTGTTTCATGAGCAGAGAGAGAAATATGAAGACCCGGCCAGAATTGGGGCATTTCTGGAAACCCTGATCACCGAAACCTGCCGGGCGTTGGAAGTGCAAATTGTCGATAGTGGCGGTGATTCATGGTCTGTCGATTCAGGAGAGTCGTTCTCACTGTGGCTTTCTTCCCATCCAGGAGAACTATCCATTAACCCGCAGCCCCATGAGGATGAGACCTCTTTGCGTGGCTTGCTGTATGAGCTCATCACCTGTGAGAGCGTGAAAACTGTTTTAAGGAGAACCGACT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   962 GenBank   WP_001326173
Name   TraI_pKp_Goe_473-2 insolico UniProt ID   _
Length   990 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 990 a.a.        Molecular weight: 109356.90 Da        Isoelectric Point: 4.9395

>WP_001326173.1 MULTISPECIES: MobH family relaxase [Gammaproteobacteria]
MLKALNKLFGGRSGVIETAPSVRVLPLKDVEDEEIPRYPPFAKGLPVAPLDKILATQAELIEKVRNSLGF
TVDDFNRLVLPVIQRYAAFVHLLPASESHHHRGAGGLFRHGLEVAFWAAQASESVIFSIEGTPRERRDNE
PRWRLASCFSGLLHDVGKPLSDVSITDKDGSITWNPYSESLHDWAHRHEIDRYFIRWRDKRHKRHEQFSL
LAVDRIIPAETREFLSKSGPSIMEAMLEAISGTSVNQPVTKLMLRADQESVSRDLRQSRLDVDEFSYGVP
VERYVFDAIRRLVKTGKWKVNEPGAKVWHLNQGVFIAWKQLGDLYDLISHDKIPGIPRDPDTLADILIER
GFAVPNTVQEKGERAYYRYWEVLPEMLQEAAGSVKILMLRLESNDLVFTTEPPAAVAAEVVGDVEDAEIE
FVDPEEVDDDQEEDVSALNDDMLAAEQEAEKALAGLGFGDAMEMLKSTSDAVEEKPEQKDAGSTESSKPD
AGKKGKPQSKPGKAKPKSDTEKQPHKPEAKEDLSPQDIAKNAPPLANDNPLQALKDVGGGLGDIDFPFDA
FSASAETASTDATNSEIPDVAMPGKQEKQPKQDFVPQEQNSLQGDDFPMFGSSDEPPSWAIEPLPMLTDA
PEQTTPAPAMPPTDKPNLHEKDAKTLLVEMLAGYGEASALLEQAIMPVLEGKTTLGEVLCLMKGQAVILY
PDGARSLGAPSEVLSKLSHANAIVPDPIMPGRKVRDFSGVKAIVLAEQLSDAVVAAIKDAEASMGGYQDA
FELVSPPGLDASKNKSAPKQQSRKKAQQQKPEVNAGKPSPEQKAKGKDSQPQQKEKKVDVTSPVEEPQRQ
PVQEKQNVARLPKREVQPVAPEPKVEREKELGHVEVREREEPEVREFEPPKAKTNPKDINAEDFLPSGVT
PQKALQMLKDMIQKRSGRWLVTPVLEEDGCLVTSDKAFDMIAGENIGISKHILCGMLSRAQRRPLLKKRQ
GKLYLEVNET

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(51-357)


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 18324..27414

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BB750_RS33415 13950..14147 + 198 Protein_18 mercuric resistance transcriptional repressor MerD -
BB750_RS29475 (BB750_29475) 14201..14905 - 705 WP_001067855 IS6-like element IS26 family transposase -
BB750_RS29480 (BB750_29480) 14970..15143 + 174 Protein_20 transcriptional regulator MerD -
BB750_RS29485 (BB750_29485) 15140..15376 + 237 WP_001087810 broad-spectrum mercury transporter MerE -
BB750_RS29490 (BB750_29490) 15412..16047 + 636 Protein_22 EAL domain-containing protein -
BB750_RS33420 16159..16362 + 204 WP_024198696 DUF3330 domain-containing protein -
BB750_RS29495 (BB750_29495) 16488..17024 + 537 WP_029404405 recombinase family protein -
BB750_RS29510 (BB750_29510) 17075..17772 + 698 WP_095033700 IS1-like element IS1B family transposase -
BB750_RS29515 (BB750_29515) 17928..18320 - 393 WP_000479535 TraA family conjugative transfer protein -
BB750_RS29520 (BB750_29520) 18324..18902 - 579 WP_000793435 type IV conjugative transfer system lipoprotein TraV traV
BB750_RS29525 (BB750_29525) 18899..20212 - 1314 WP_024131605 TraB/VirB10 family protein traB
BB750_RS29530 (BB750_29530) 20212..21129 - 918 WP_000794249 type-F conjugative transfer system secretin TraK traK
BB750_RS29535 (BB750_29535) 21113..21739 - 627 WP_001049717 TraE/TraK family type IV conjugative transfer system protein traE
BB750_RS29540 (BB750_29540) 21736..22017 - 282 WP_000805625 type IV conjugative transfer system protein TraL traL
BB750_RS29545 (BB750_29545) 22162..22527 - 366 WP_001052530 hypothetical protein -
BB750_RS33155 22539..22682 - 144 WP_001275801 hypothetical protein -
BB750_RS29550 (BB750_29550) 22863..23525 - 663 WP_001231464 hypothetical protein -
BB750_RS29555 (BB750_29555) 23525..23902 - 378 WP_000869297 hypothetical protein -
BB750_RS29560 (BB750_29560) 23912..24358 - 447 WP_000122507 hypothetical protein -
BB750_RS29565 (BB750_29565) 24368..24997 - 630 WP_000743449 DUF4400 domain-containing protein tfc7
BB750_RS29570 (BB750_29570) 24954..25499 - 546 WP_000228720 hypothetical protein -
BB750_RS29575 (BB750_29575) 25549..27414 - 1866 WP_000178857 conjugative transfer system coupling protein TraD virb4
BB750_RS29580 (BB750_29580) 27411..30383 - 2973 WP_001326173 MobH family relaxase -
BB750_RS29585 (BB750_29585) 30551..31168 + 618 WP_001249395 hypothetical protein -
BB750_RS29590 (BB750_29590) 31150..31383 + 234 WP_001191890 hypothetical protein -

Host bacterium

ID   1350 GenBank   NZ_CP018689
Plasmid name   pKp_Goe_473-2 Incompatibility group   IncA/C2
Plasmid size   96070 bp Coordinate of oriT [Strand]   24998..25548 [-]
Host baterium   Klebsiella pneumoniae strain Kp_Goe_149473

Cargo genes

Drug resistance gene   sul2, aph(3'')-Ib, aph(6)-Id, tet(A), floR, sul1, qacE, aac(6')-Ib
Virulence gene   -
Metal resistance gene   merR, merT, merP, merA, merB, merE
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -