Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100887
Name   oriT_pKp_Goe_070-3 in_silico
Organism   Klebsiella pneumoniae strain Kp_Goe_71070
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NZ_CP018453 (122..302 [+], 181 nt)
oriT length   181 nt
IRs (inverted repeats)      10..17, 21..28  (TTCGGGGC..GCCCTGAA)
Location of nic site      61..62
Conserved sequence flanking the
  nic site    		  CTGG|CTAA
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 181 nt

>oriT_pKp_Goe_070-3
TTAGCGGGTTTCGGGGCGCAGCCCTGAACCAGTCACGTAGCGCTAGCGGAGTGTATACTGGCTAAATATGTTACGGCGGAGTGGTAATGTATGAAAGTGCTTCATGTGGGAAGGGGAAAATGTGGCTGACGGGCGTAAGTGCAACATGCTGGTAAAGGTGAAATGACGCCTCCTCGCTCAC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   958 GenBank   WP_071890506
Name   Nuclease_pKp_Goe_070-3 insolico UniProt ID   _
Length   502 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 502 a.a.        Molecular weight: 54878.09 Da        Isoelectric Point: 6.8809

>WP_071890506.1 relaxase/mobilization nuclease domain-containing protein [Klebsiella pneumoniae]
MIVKFHPRGRGGGAGPVDYLLGKDRQREGATVLQGKPEEVRELIDASPYAKKYTSGVLSFAEAELLPGQR
EQIMASFERVLMPGLDKDQYSILWVEHTDKGRLELNFLIPNTELLTGKRLQPYYDRADRPRIDAWQTVVN
GRLGLHDPNAPENRRLLVTPSALPETKLEAAQAITRGLLALASSGELKTREDVTGALTAAGFEVVRTTKN
SISIADPDGGRNIRLKGAIYEQSFSAGEGLGAAIESAAAEYRRDAESRIQRAREICKSGTERKREENQRR
HQRPRAGYDRGDAVEPPERAAYGRPDVADHHPGLGTADRHEREHSVVAGAADHRELPDYPGAGADAGHVV
GEKPRRSAVALRGEETVLRQSEPESGSLRGGRELDGTGEEIAHDGTGKTVADRIRAATSGLLAKAGRVGE
RLRGMAEDVWSYATGERGAERARHALESAGGELEQAVTAFEPVVQRHEMAVAAERAYEQRQHEKELAEAR
SRQRSYNGPTLG

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(57-237)


  Protein structure



No available structure.




Host bacterium

ID   1346 GenBank   NZ_CP018453
Plasmid name   pKp_Goe_070-3 Incompatibility group   Col440II
Plasmid size   4075 bp Coordinate of oriT [Strand]   122..302 [+]
Host baterium   Klebsiella pneumoniae strain Kp_Goe_71070

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -