Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 100882 |
| Name | oriT_pKp_Goe_414-5  |
| Organism | Klebsiella pneumoniae isolate Kp_Goe_154414 |
| Sequence Completeness | core |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP018342 (8042..8147 [+], 106 nt) |
| oriT length | 106 nt |
| IRs (inverted repeats) | 18..35, 41..58 (GTTTTTGGAGTACCGCCG..CGGCGGCCGTCCAAAAAC) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | predicted by the oriTfinder |
oriT sequence
Download Length: 106 nt
>oriT_pKp_Goe_414-5
AGATAGCTAACCTCGTTAGGGGGTGTCGGGGCTTGCCCTGACCAAGACGTTTTTGGACGGCCGCCGCGTGTCGGCGGTACTCCAAAAACACATCTTGTCCCGTACT
AGATAGCTAACCTCGTTAGGGGGTGTCGGGGCTTGCCCTGACCAAGACGTTTTTGGACGGCCGCCGCGTGTCGGCGGTACTCCAAAAACACATCTTGTCCCGTACT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 953 | GenBank | WP_004187323 |
| Name | PutativerelaxaseofpKp_Goe_414-5 |
UniProt ID | _ |
| Length | 659 a.a. | PDB ID | |
| Note | putative relaxase | ||
Relaxase protein sequence
Download Length: 659 a.a. Molecular weight: 75179.89 Da Isoelectric Point: 9.9948
>WP_004187323.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 41492..63204
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| BB788_RS30500 (BB788_30210) | 37165..37962 | - | 798 | WP_015059991 | OXA-48 family carbapenem-hydrolyzing class D beta-lactamase OXA-48 | - |
| BB788_RS30515 (BB788_30220) | 38181..38878 | + | 698 | WP_095033700 | IS1-like element IS1B family transposase | - |
| BB788_RS30520 (BB788_30225) | 38875..40080 | + | 1206 | WP_025987686 | IS4-like element IS10A family transposase | - |
| BB788_RS30525 (BB788_30230) | 40095..40529 | - | 435 | Protein_60 | CPBP family intramembrane glutamate endopeptidase | - |
| BB788_RS30530 (BB788_30235) | 40609..40992 | + | 384 | WP_019725042 | DUF1496 domain-containing protein | - |
| BB788_RS30535 (BB788_30240) | 41097..41492 | + | 396 | WP_004187436 | lytic transglycosylase domain-containing protein | - |
| BB788_RS30540 (BB788_30245) | 41492..42799 | + | 1308 | WP_015059988 | hypothetical protein | trbA |
| BB788_RS30545 (BB788_30250) | 42810..43760 | + | 951 | WP_004206886 | DsbC family protein | trbB |
| BB788_RS30550 (BB788_30255) | 43773..45860 | + | 2088 | WP_004206885 | conjugal transfer protein TrbC | - |
| BB788_RS33220 | 45911..46006 | - | 96 | WP_004206884 | DinQ-like type I toxin DqlB | - |
| BB788_RS30555 (BB788_30260) | 47229..48284 | - | 1056 | WP_015060006 | plasmid replication initiator RepA | - |
| BB788_RS30560 (BB788_30265) | 48580..48822 | - | 243 | WP_023893611 | IncL/M type plasmid replication protein RepC | - |
| BB788_RS30565 (BB788_30270) | 48891..49544 | - | 654 | WP_015060005 | hypothetical protein | - |
| BB788_RS30570 (BB788_30275) | 49546..51741 | - | 2196 | WP_015062834 | DotA/TraY family protein | traY |
| BB788_RS30575 (BB788_30280) | 51734..52330 | - | 597 | WP_015060003 | hypothetical protein | - |
| BB788_RS30580 (BB788_30285) | 52327..53535 | - | 1209 | WP_011091082 | conjugal transfer protein TraW | traW |
| BB788_RS30585 (BB788_30290) | 53532..56582 | - | 3051 | WP_004187482 | hypothetical protein | traU |
| BB788_RS30590 (BB788_30295) | 56579..57073 | - | 495 | WP_004187480 | hypothetical protein | - |
| BB788_RS30595 (BB788_30300) | 57119..57508 | - | 390 | WP_004187479 | DUF6750 family protein | traR |
| BB788_RS30600 (BB788_30305) | 57525..58055 | - | 531 | WP_004187478 | conjugal transfer protein TraQ | traQ |
| BB788_RS30605 (BB788_30310) | 58079..58783 | - | 705 | WP_015060002 | conjugal transfer protein TraP | traP |
| BB788_RS30610 (BB788_30315) | 58795..60144 | - | 1350 | WP_004187474 | conjugal transfer protein TraO | traO |
| BB788_RS30615 (BB788_30320) | 60156..61307 | - | 1152 | WP_004187471 | DotH/IcmK family type IV secretion protein | traN |
| BB788_RS30620 (BB788_30325) | 61316..62029 | - | 714 | WP_004187467 | DotI/IcmL family type IV secretion protein | traM |
| BB788_RS30625 (BB788_30330) | 62097..62507 | + | 411 | WP_004187465 | H-NS family nucleoid-associated regulatory protein | - |
| BB788_RS30630 (BB788_30335) | 62536..62691 | + | 156 | WP_172693602 | Hha/YmoA family nucleoid-associated regulatory protein | - |
| BB788_RS30635 (BB788_30340) | 62692..63204 | - | 513 | WP_011091071 | hypothetical protein | traL |
Host bacterium
| ID | 1341 | GenBank | NZ_CP018342 |
| Plasmid name | pKp_Goe_414-5 | Incompatibility group | IncL/M |
| Plasmid size | 63588 bp | Coordinate of oriT [Strand] | 8042..8147 [+] |
| Host baterium | Klebsiella pneumoniae isolate Kp_Goe_154414 |
Cargo genes
| Drug resistance gene | blaOXA-48 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrIIA8 |