Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 100880 |
| Name | oriT_pKp_Goe_579-2  |
| Organism | Klebsiella pneumoniae strain Kp_Goe_822579 |
| Sequence Completeness | intact |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP018318 (50475..51025 [-], 551 nt) |
| oriT length | 551 nt |
| IRs (inverted repeats) | IR1: 62..70, 71..79 (AACCCTTTC..GACAGGGTT) IR2: 143..150, 154..161 (TGGCCTGC..GGAGGCCA) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | predicted by the oriTfinder |
oriT sequence
Download Length: 551 nt
>oriT_pKp_Goe_579-2
GAGCAGAGCTATGTGTGACAAGAAGTATAGAAATTACGAGGTAGCCATCATGGTCGATGTGAACCCTTTCGACAGGGTTATGAATGAATTGAAAAGTCGTGGCCGCAAGAACGCTCACATCCTGAGCATCCTCCAATTCGACTGGCCTGCATCGGAGGCCATCATCGAGAAGCTGAGCTGCTACATCACAGACGGGATTAAGGCTAATCAGGAGCCTGTGATTTACCCGATCATTGAAGAAGCTCTGCATCGCTACAGCCAGCTCGTGTTTCATGAGCAGAGAGAGAAATATGAAGACCCGGCCAGAATTGGGGCATTTCTGGAAACCCTGATCACCGAAACCTGCCGGGCGTTGGAAGTGCAAATTGTCGATAGTGGCGGTGATTCATGGTCTGTCGATTCAGGAGAGTCGTTCTCACTGTGGCTTTCTTCCCATCCAGGAGAACTATCCATTAACCCGCAGCCCCATGAGGATGAGACCTCTTTGCGTGGCTTGCTGTATGAGCTCATCACCTGTGAGAGCGTGAAAACTGTTTTAAGGAGAACCGACT
GAGCAGAGCTATGTGTGACAAGAAGTATAGAAATTACGAGGTAGCCATCATGGTCGATGTGAACCCTTTCGACAGGGTTATGAATGAATTGAAAAGTCGTGGCCGCAAGAACGCTCACATCCTGAGCATCCTCCAATTCGACTGGCCTGCATCGGAGGCCATCATCGAGAAGCTGAGCTGCTACATCACAGACGGGATTAAGGCTAATCAGGAGCCTGTGATTTACCCGATCATTGAAGAAGCTCTGCATCGCTACAGCCAGCTCGTGTTTCATGAGCAGAGAGAGAAATATGAAGACCCGGCCAGAATTGGGGCATTTCTGGAAACCCTGATCACCGAAACCTGCCGGGCGTTGGAAGTGCAAATTGTCGATAGTGGCGGTGATTCATGGTCTGTCGATTCAGGAGAGTCGTTCTCACTGTGGCTTTCTTCCCATCCAGGAGAACTATCCATTAACCCGCAGCCCCATGAGGATGAGACCTCTTTGCGTGGCTTGCTGTATGAGCTCATCACCTGTGAGAGCGTGAAAACTGTTTTAAGGAGAACCGACT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 951 | GenBank | WP_001326173 |
| Name | TraI_pKp_Goe_579-2 |
UniProt ID | _ |
| Length | 990 a.a. | PDB ID | |
| Note | putative relaxase | ||
Relaxase protein sequence
Download Length: 990 a.a. Molecular weight: 109356.90 Da Isoelectric Point: 4.9395
>WP_001326173.1 MULTISPECIES: MobH family relaxase [Gammaproteobacteria]
MLKALNKLFGGRSGVIETAPSVRVLPLKDVEDEEIPRYPPFAKGLPVAPLDKILATQAELIEKVRNSLGF
TVDDFNRLVLPVIQRYAAFVHLLPASESHHHRGAGGLFRHGLEVAFWAAQASESVIFSIEGTPRERRDNE
PRWRLASCFSGLLHDVGKPLSDVSITDKDGSITWNPYSESLHDWAHRHEIDRYFIRWRDKRHKRHEQFSL
LAVDRIIPAETREFLSKSGPSIMEAMLEAISGTSVNQPVTKLMLRADQESVSRDLRQSRLDVDEFSYGVP
VERYVFDAIRRLVKTGKWKVNEPGAKVWHLNQGVFIAWKQLGDLYDLISHDKIPGIPRDPDTLADILIER
GFAVPNTVQEKGERAYYRYWEVLPEMLQEAAGSVKILMLRLESNDLVFTTEPPAAVAAEVVGDVEDAEIE
FVDPEEVDDDQEEDVSALNDDMLAAEQEAEKALAGLGFGDAMEMLKSTSDAVEEKPEQKDAGSTESSKPD
AGKKGKPQSKPGKAKPKSDTEKQPHKPEAKEDLSPQDIAKNAPPLANDNPLQALKDVGGGLGDIDFPFDA
FSASAETASTDATNSEIPDVAMPGKQEKQPKQDFVPQEQNSLQGDDFPMFGSSDEPPSWAIEPLPMLTDA
PEQTTPAPAMPPTDKPNLHEKDAKTLLVEMLAGYGEASALLEQAIMPVLEGKTTLGEVLCLMKGQAVILY
PDGARSLGAPSEVLSKLSHANAIVPDPIMPGRKVRDFSGVKAIVLAEQLSDAVVAAIKDAEASMGGYQDA
FELVSPPGLDASKNKSAPKQQSRKKAQQQKPEVNAGKPSPEQKAKGKDSQPQQKEKKVDVTSPVEEPQRQ
PVQEKQNVARLPKREVQPVAPEPKVEREKELGHVEVREREEPEVREFEPPKAKTNPKDINAEDFLPSGVT
PQKALQMLKDMIQKRSGRWLVTPVLEEDGCLVTSDKAFDMIAGENIGISKHILCGMLSRAQRRPLLKKRQ
GKLYLEVNET
MLKALNKLFGGRSGVIETAPSVRVLPLKDVEDEEIPRYPPFAKGLPVAPLDKILATQAELIEKVRNSLGF
TVDDFNRLVLPVIQRYAAFVHLLPASESHHHRGAGGLFRHGLEVAFWAAQASESVIFSIEGTPRERRDNE
PRWRLASCFSGLLHDVGKPLSDVSITDKDGSITWNPYSESLHDWAHRHEIDRYFIRWRDKRHKRHEQFSL
LAVDRIIPAETREFLSKSGPSIMEAMLEAISGTSVNQPVTKLMLRADQESVSRDLRQSRLDVDEFSYGVP
VERYVFDAIRRLVKTGKWKVNEPGAKVWHLNQGVFIAWKQLGDLYDLISHDKIPGIPRDPDTLADILIER
GFAVPNTVQEKGERAYYRYWEVLPEMLQEAAGSVKILMLRLESNDLVFTTEPPAAVAAEVVGDVEDAEIE
FVDPEEVDDDQEEDVSALNDDMLAAEQEAEKALAGLGFGDAMEMLKSTSDAVEEKPEQKDAGSTESSKPD
AGKKGKPQSKPGKAKPKSDTEKQPHKPEAKEDLSPQDIAKNAPPLANDNPLQALKDVGGGLGDIDFPFDA
FSASAETASTDATNSEIPDVAMPGKQEKQPKQDFVPQEQNSLQGDDFPMFGSSDEPPSWAIEPLPMLTDA
PEQTTPAPAMPPTDKPNLHEKDAKTLLVEMLAGYGEASALLEQAIMPVLEGKTTLGEVLCLMKGQAVILY
PDGARSLGAPSEVLSKLSHANAIVPDPIMPGRKVRDFSGVKAIVLAEQLSDAVVAAIKDAEASMGGYQDA
FELVSPPGLDASKNKSAPKQQSRKKAQQQKPEVNAGKPSPEQKAKGKDSQPQQKEKKVDVTSPVEEPQRQ
PVQEKQNVARLPKREVQPVAPEPKVEREKELGHVEVREREEPEVREFEPPKAKTNPKDINAEDFLPSGVT
PQKALQMLKDMIQKRSGRWLVTPVLEEDGCLVTSDKAFDMIAGENIGISKHILCGMLSRAQRRPLLKKRQ
GKLYLEVNET
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 43801..52891
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| BB744_RS33520 | 39427..39624 | + | 198 | Protein_45 | mercuric resistance transcriptional repressor MerD | - |
| BB744_RS30515 (BB744_05717) | 39678..40382 | - | 705 | WP_001067855 | IS6-like element IS26 family transposase | - |
| BB744_RS30520 | 40447..40620 | + | 174 | Protein_47 | transcriptional regulator MerD | - |
| BB744_RS30525 (BB744_05718) | 40617..40853 | + | 237 | WP_001087810 | broad-spectrum mercury transporter MerE | - |
| BB744_RS30530 (BB744_05719) | 40889..41524 | + | 636 | Protein_49 | EAL domain-containing protein | - |
| BB744_RS33525 | 41636..41839 | + | 204 | WP_024198696 | DUF3330 domain-containing protein | - |
| BB744_RS30535 (BB744_05720) | 41965..42501 | + | 537 | WP_029404405 | recombinase family protein | - |
| BB744_RS30550 (BB744_05722) | 42552..43249 | + | 698 | WP_095033700 | IS1-like element IS1B family transposase | - |
| BB744_RS30555 (BB744_05723) | 43405..43797 | - | 393 | WP_000479535 | TraA family conjugative transfer protein | - |
| BB744_RS30560 (BB744_05724) | 43801..44379 | - | 579 | WP_000793435 | type IV conjugative transfer system lipoprotein TraV | traV |
| BB744_RS30565 (BB744_05725) | 44376..45689 | - | 1314 | WP_024131605 | TraB/VirB10 family protein | traB |
| BB744_RS30570 (BB744_05726) | 45689..46606 | - | 918 | WP_000794249 | type-F conjugative transfer system secretin TraK | traK |
| BB744_RS30575 (BB744_05727) | 46590..47216 | - | 627 | WP_001049717 | TraE/TraK family type IV conjugative transfer system protein | traE |
| BB744_RS30580 (BB744_05728) | 47213..47494 | - | 282 | WP_000805625 | type IV conjugative transfer system protein TraL | traL |
| BB744_RS30585 (BB744_05729) | 47639..48004 | - | 366 | WP_001052530 | hypothetical protein | - |
| BB744_RS33235 (BB744_05730) | 48016..48159 | - | 144 | WP_001275801 | hypothetical protein | - |
| BB744_RS30590 (BB744_05731) | 48340..49002 | - | 663 | WP_001231464 | hypothetical protein | - |
| BB744_RS30595 (BB744_05732) | 49002..49379 | - | 378 | WP_000869297 | hypothetical protein | - |
| BB744_RS30600 (BB744_05733) | 49389..49835 | - | 447 | WP_000122507 | hypothetical protein | - |
| BB744_RS30605 (BB744_05734) | 49845..50474 | - | 630 | WP_000743449 | DUF4400 domain-containing protein | tfc7 |
| BB744_RS30610 (BB744_05735) | 50431..50976 | - | 546 | WP_000228720 | hypothetical protein | - |
| BB744_RS30615 (BB744_05736) | 51026..52891 | - | 1866 | WP_000178857 | conjugative transfer system coupling protein TraD | virb4 |
| BB744_RS30620 (BB744_05737) | 52888..55860 | - | 2973 | WP_001326173 | MobH family relaxase | - |
| BB744_RS30625 (BB744_05738) | 56028..56645 | + | 618 | WP_001249395 | hypothetical protein | - |
| BB744_RS30630 | 56627..56860 | + | 234 | WP_001191890 | hypothetical protein | - |
Host bacterium
| ID | 1339 | GenBank | NZ_CP018318 |
| Plasmid name | pKp_Goe_579-2 | Incompatibility group | IncA/C2 |
| Plasmid size | 96082 bp | Coordinate of oriT [Strand] | 50475..51025 [-] |
| Host baterium | Klebsiella pneumoniae strain Kp_Goe_822579 |
Cargo genes
| Drug resistance gene | sul2, aph(3'')-Ib, aph(6)-Id, tet(A), floR, sul1, qacE, aac(6')-Ib |
| Virulence gene | - |
| Metal resistance gene | merR, merT, merP, merA, merB, merE |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |