Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 100879 |
| Name | oriT_pKp_Goe_579-3  |
| Organism | Klebsiella pneumoniae strain Kp_Goe_822579 |
| Sequence Completeness | core |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP018315 (14097..14202 [+], 106 nt) |
| oriT length | 106 nt |
| IRs (inverted repeats) | 18..35, 41..58 (GTTTTTGGAGTACCGCCG..CGGCGGCCGTCCAAAAAC) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | predicted by the oriTfinder |
oriT sequence
Download Length: 106 nt
>oriT_pKp_Goe_579-3
AGATAGCTAACCTCGTTAGGGGGTGTCGGGGCTTGCCCTGACCAAGACGTTTTTGGACGGCCGCCGCGTGTCGGCGGTACTCCAAAAACACATCTTGTCCCGTACT
AGATAGCTAACCTCGTTAGGGGGTGTCGGGGCTTGCCCTGACCAAGACGTTTTTGGACGGCCGCCGCGTGTCGGCGGTACTCCAAAAACACATCTTGTCCCGTACT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 950 | GenBank | WP_004187323 |
| Name | PutativerelaxaseofpKp_Goe_579-3 |
UniProt ID | _ |
| Length | 659 a.a. | PDB ID | |
| Note | putative relaxase | ||
Relaxase protein sequence
Download Length: 659 a.a. Molecular weight: 75179.89 Da Isoelectric Point: 9.9948
>WP_004187323.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 545..11301
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| BB744_RS29355 (BB744_05511) | 545..1249 | - | 705 | WP_015060002 | conjugal transfer protein TraP | traP |
| BB744_RS29360 (BB744_05512) | 1261..2610 | - | 1350 | WP_004187474 | conjugal transfer protein TraO | traO |
| BB744_RS29365 (BB744_05513) | 2622..3773 | - | 1152 | WP_004187471 | DotH/IcmK family type IV secretion protein | traN |
| BB744_RS29370 (BB744_05514) | 3782..4495 | - | 714 | WP_004187467 | DotI/IcmL family type IV secretion protein | traM |
| BB744_RS29375 (BB744_05515) | 4563..4973 | + | 411 | WP_004187465 | H-NS family nucleoid-associated regulatory protein | - |
| BB744_RS29380 | 5002..5157 | + | 156 | WP_172693602 | Hha/YmoA family nucleoid-associated regulatory protein | - |
| BB744_RS29385 (BB744_05516) | 5158..5670 | - | 513 | WP_011091071 | hypothetical protein | traL |
| BB744_RS33490 (BB744_05517) | 5636..9073 | - | 3438 | WP_015586048 | LPD7 domain-containing protein | - |
| BB744_RS29395 (BB744_05518) | 9098..9358 | - | 261 | WP_004187310 | IcmT/TraK family protein | traK |
| BB744_RS29400 (BB744_05519) | 9348..10511 | - | 1164 | WP_004187313 | plasmid transfer ATPase TraJ | virB11 |
| BB744_RS29405 (BB744_05520) | 10522..11301 | - | 780 | WP_004187315 | type IV secretory system conjugative DNA transfer family protein | traI |
| BB744_RS29410 (BB744_05521) | 11298..11798 | - | 501 | WP_004187320 | DotD/TraH family lipoprotein | - |
| BB744_RS29415 (BB744_05522) | 11812..13791 | - | 1980 | WP_004187323 | TraI/MobA(P) family conjugative relaxase | - |
| BB744_RS33495 (BB744_05523) | 13778..14305 | - | 528 | WP_172740549 | plasmid mobilization protein MobA | - |
| BB744_RS29425 (BB744_05524) | 14340..14735 | + | 396 | WP_019725163 | hypothetical protein | - |
| BB744_RS29430 (BB744_05526) | 15237..15773 | - | 537 | WP_004187332 | hypothetical protein | - |
| BB744_RS29435 (BB744_05527) | 15906..16217 | - | 312 | WP_004187333 | hypothetical protein | - |
Region 2: 123..11301
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| BB744_RS29355 (BB744_05511) | 545..1249 | - | 705 | WP_015060002 | conjugal transfer protein TraP | traP |
| BB744_RS29360 (BB744_05512) | 1261..2610 | - | 1350 | WP_004187474 | conjugal transfer protein TraO | traO |
| BB744_RS29365 (BB744_05513) | 2622..3773 | - | 1152 | WP_004187471 | DotH/IcmK family type IV secretion protein | traN |
| BB744_RS29370 (BB744_05514) | 3782..4495 | - | 714 | WP_004187467 | DotI/IcmL family type IV secretion protein | traM |
| BB744_RS29375 (BB744_05515) | 4563..4973 | + | 411 | WP_004187465 | H-NS family nucleoid-associated regulatory protein | - |
| BB744_RS29380 | 5002..5157 | + | 156 | WP_172693602 | Hha/YmoA family nucleoid-associated regulatory protein | - |
| BB744_RS29385 (BB744_05516) | 5158..5670 | - | 513 | WP_011091071 | hypothetical protein | traL |
| BB744_RS33490 (BB744_05517) | 5636..9073 | - | 3438 | WP_015586048 | LPD7 domain-containing protein | - |
| BB744_RS29395 (BB744_05518) | 9098..9358 | - | 261 | WP_004187310 | IcmT/TraK family protein | traK |
| BB744_RS29400 (BB744_05519) | 9348..10511 | - | 1164 | WP_004187313 | plasmid transfer ATPase TraJ | virB11 |
| BB744_RS29405 (BB744_05520) | 10522..11301 | - | 780 | WP_004187315 | type IV secretory system conjugative DNA transfer family protein | traI |
| BB744_RS29410 (BB744_05521) | 11298..11798 | - | 501 | WP_004187320 | DotD/TraH family lipoprotein | - |
| BB744_RS29415 (BB744_05522) | 11812..13791 | - | 1980 | WP_004187323 | TraI/MobA(P) family conjugative relaxase | - |
| BB744_RS33495 (BB744_05523) | 13778..14305 | - | 528 | WP_172740549 | plasmid mobilization protein MobA | - |
| BB744_RS29425 (BB744_05524) | 14340..14735 | + | 396 | WP_019725163 | hypothetical protein | - |
| BB744_RS29430 (BB744_05526) | 15237..15773 | - | 537 | WP_004187332 | hypothetical protein | - |
| BB744_RS29435 (BB744_05527) | 15906..16217 | - | 312 | WP_004187333 | hypothetical protein | - |
Region 3: 47592..63563
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| BB744_RS29675 (BB744_05576) | 43220..44017 | - | 798 | WP_015059991 | OXA-48 family carbapenem-hydrolyzing class D beta-lactamase OXA-48 | - |
| BB744_RS29690 (BB744_05579) | 44236..44933 | + | 698 | WP_095033700 | IS1-like element IS1B family transposase | - |
| BB744_RS29695 (BB744_05580) | 44930..46135 | + | 1206 | WP_025987686 | IS4-like element IS10A family transposase | - |
| BB744_RS29700 (BB744_05581) | 46150..46584 | - | 435 | Protein_67 | CPBP family intramembrane glutamate endopeptidase | - |
| BB744_RS29705 (BB744_05582) | 46664..47047 | + | 384 | WP_019725042 | DUF1496 domain-containing protein | - |
| BB744_RS29710 (BB744_05583) | 47152..47547 | + | 396 | WP_004187436 | lytic transglycosylase domain-containing protein | - |
| BB744_RS29715 (BB744_05584) | 47592..48854 | + | 1263 | WP_004206887 | hypothetical protein | trbA |
| BB744_RS29720 (BB744_05585) | 48865..49815 | + | 951 | WP_004206886 | DsbC family protein | trbB |
| BB744_RS29725 (BB744_05586) | 49828..51915 | + | 2088 | WP_004206885 | conjugal transfer protein TrbC | - |
| BB744_RS33645 | 51966..52061 | - | 96 | WP_004206884 | DinQ-like type I toxin DqlB | - |
| BB744_RS29730 (BB744_05587) | 53284..54339 | - | 1056 | WP_015060006 | plasmid replication initiator RepA | - |
| BB744_RS29735 (BB744_05589) | 54635..54865 | - | 231 | WP_011091085 | IncL/M type plasmid replication protein RepC | - |
| BB744_RS29740 (BB744_05590) | 54946..55599 | - | 654 | WP_015060005 | hypothetical protein | - |
| BB744_RS29745 (BB744_05591) | 55601..57796 | - | 2196 | WP_015062834 | DotA/TraY family protein | traY |
| BB744_RS29750 (BB744_05592) | 57789..58385 | - | 597 | WP_032419542 | hypothetical protein | - |
| BB744_RS29755 (BB744_05593) | 58382..59590 | - | 1209 | WP_011091082 | conjugal transfer protein TraW | traW |
| BB744_RS29760 (BB744_05594) | 59587..62637 | - | 3051 | WP_004187482 | hypothetical protein | traU |
| BB744_RS29765 (BB744_05595) | 62634..63128 | - | 495 | WP_004187480 | hypothetical protein | - |
| BB744_RS29770 (BB744_05596) | 63174..63563 | - | 390 | WP_004187479 | DUF6750 family protein | traR |
Host bacterium
| ID | 1338 | GenBank | NZ_CP018315 |
| Plasmid name | pKp_Goe_579-3 | Incompatibility group | IncL/M |
| Plasmid size | 63587 bp | Coordinate of oriT [Strand] | 14097..14202 [+] |
| Host baterium | Klebsiella pneumoniae strain Kp_Goe_822579 |
Cargo genes
| Drug resistance gene | blaOXA-48 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |