Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100877
Name   oriT_pHSL2 in_silico
Organism   Tardibacter chloracetimidivorans strain JJ-A5
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NZ_CP018223 (27601..27790 [-], 190 nt)
oriT length   190 nt
IRs (inverted repeats)      IR1: 19..38, 39..58  (AACCGTGAAGCGCGATCCGA..TGGGATTGAGCGTAACGGTT)
  IR2: 117..128, 131..142  (AAAGCGGTGGCA..TGCCACCGCTCT)
Location of nic site      86..87
Conserved sequence flanking the
  nic site    		  TATCCTG|C
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 190 nt

>oriT_pHSL2
GTGGGGTAGGTATCTCCCAACCGTGAAGCGCGATCCGATGGGATTGAGCGTAACGGTTAACATTAGCCGCCGCAAGGCTTATCCTGCCATACAGTAGATACCTACCCCACAAACAAAAAGCGGTGGCAACTGCCACCGCTCTAAATTCCACGTCGCCTCGATGCTGAGTGAACGTGGACGGTAAAAGCTA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   948 GenBank   WP_056379678
Name   VirD2_pHSL2 insolico UniProt ID   A0A1L4A0K2
Length   347 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 347 a.a.        Molecular weight: 38691.33 Da        Isoelectric Point: 11.2122

>WP_056379678.1 MULTISPECIES: relaxase/mobilization nuclease domain-containing protein [Sphingomonadaceae]
MSDFDSSFEAGQLAALFKPKLTSDPNRRGADMLLRSLSSRRAGQGGSTRARLARVVSRAPEVMVKVTGRP
KGKNHAAAHFDYIGRKGDVPLETRDGDILTDKEDRAELARDWGDPVYWRDNSTVAAVSMVFSMPSGTDPD
KVLSAVREVARSEIGHEWDYVLALHTDTPRPHVHVTVAARGDTGRRFNPRPQTLHHYRERFAEELRARGV
TAEATPRAARGVGRAGQSMALNRMRQRYMAGTAPAPFANQKITAAARDQLAGRSTAPDFVVRGRQAWNET
HRRYLAAAKRLEVSSDPADRQLADQVREFVGAGRTPTIHERSVAAIERKRQNERSRDRDRSREGPER

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(75-203)


  Protein structure


Source ID Structure
AlphaFold DB A0A1L4A0K2


Host bacterium

ID   1336 GenBank   NZ_CP018223
Plasmid name   pHSL2 Incompatibility group   _
Plasmid size   61559 bp Coordinate of oriT [Strand]   27601..27790 [-]
Host baterium   Tardibacter chloracetimidivorans strain JJ-A5

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -