Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 100870 |
| Name | oriT_pMR0716_ColRNAI  |
| Organism | Escherichia coli strain MRSN352231 |
| Sequence Completeness | intact |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP018108 (1647..1730 [+], 84 nt) |
| oriT length | 84 nt |
| IRs (inverted repeats) | 3..12, 16..25 (GTGTCGGGGC..GCCCTGACCC) |
| Location of nic site | 56..57 |
| Conserved sequence flanking the nic site |
CTGG|CTTA |
| Note | predicted by the oriTfinder |
oriT sequence
Download Length: 84 nt
>oriT_pMR0716_ColRNAI
GGGGTGTCGGGGCGAAGCCCTGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACCATTCGGCATCAGTGCGGATT
GGGGTGTCGGGGCGAAGCCCTGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACCATTCGGCATCAGTGCGGATT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 941 | GenBank | WP_000955999 |
| Name | MobC_pMR0716_ColRNAI |
UniProt ID | _ |
| Length | 107 a.a. | PDB ID | |
| Note | putative relaxase | ||
Relaxase protein sequence
Download Length: 107 a.a. Molecular weight: 11855.71 Da Isoelectric Point: 10.8973
>WP_000955999.1 MULTISPECIES: MobC family plasmid mobilization relaxosome protein [Bacteria]
MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTLAPPLLRQLAAIGNNLNQTARKVNSG
QWSSGDRVQVVAALMAIGDELRRLRLAVREQGARDDS
MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTLAPPLLRQLAAIGNNLNQTARKVNSG
QWSSGDRVQVVAALMAIGDELRRLRLAVREQGARDDS
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
Host bacterium
| ID | 1329 | GenBank | NZ_CP018108 |
| Plasmid name | pMR0716_ColRNAI | Incompatibility group | ColRNAI |
| Plasmid size | 5310 bp | Coordinate of oriT [Strand] | 1647..1730 [+] |
| Host baterium | Escherichia coli strain MRSN352231 |
Cargo genes
| Drug resistance gene | aph(3')-Ia |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |