Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100867
Name   oriT1_pVRE001-2 in_silico
Organism   Enterococcus faecium strain VRE001
Sequence Completeness      core
NCBI accession of oriT (coordinates [strand])   NZ_CP018072 (10249..10286 [-], 38 nt)
oriT length   38 nt
IRs (inverted repeats)      2..8, 13..19  (ACTTTAT..ATAAAGT)
Location of nic site      27..28
Conserved sequence flanking the
  nic site    		  GTG|TTA
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 38 nt

>oriT1_pVRE001-2
CACTTTATGAATATAAAGTATAGTGTGTTATACTTTAC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   939 GenBank   WP_000119405
Name   PutativerelaxaseofpVRE001-2 insolico UniProt ID   _
Length   420 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 420 a.a.        Molecular weight: 49660.33 Da        Isoelectric Point: 9.9693

>WP_000119405.1 MULTISPECIES: MobV family relaxase [Bacteria]
MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENYDLKNDKNIDYNERVKEIIESQKTGTR
KTRKDAVLVNELLVTSDRDFFEQLDPGEQKRFFEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPM
RDGKLQGKNVFNRQELLWLQDKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVK
KDEWTAYSDKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTAARDN
DRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEENKSLKSKISDLKRDVSL
IYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHDLEPKKNEFELTHNREVKKERSRDQGMSL

  Protein domains


Predicted by InterproScan.

Plasmid recombination enzyme (Mob_Pre)

(1-193)


  Protein structure



No available structure.




Host bacterium

ID   1327 GenBank   NZ_CP018072
Plasmid name   pVRE001-2 Incompatibility group   _
Plasmid size   59226 bp Coordinate of oriT [Strand]   54424..54461 [-]; 10249..10286 [-]
Host baterium   Enterococcus faecium strain VRE001

Cargo genes

Drug resistance gene   tetracycline resistance
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -